A system for controlling population of a biological species includes a population of genetically-modified individuals of the biological species, where both males and females in the genetically-modified population carry two mutations. The first mutation is a repressible genetic mutation that results in death of a juvenile individual of the first sex when the juvenile individual of the first sex comprises the repressible lethal mutation and is reared in the absence of a repressor or causes an individual to be sterile when reared in the absence of a repressor. The second mutation is an underdominant genetic mutation.

The present disclosure provides, at least, sterile fish and methods for producing sterile fish.

Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.

Fish embryos may be successfully vaccinated or therapeutically treated if the therapeutic agent is injected into the yolk sac. Therapeutic agents may be directly injected or released from microspheres and enter the circulation and tissues. Injection into the yolk sac, combined with the use of carriers, allows for a continued, controlled release of therapeutic agents and processing of antigens. Fish vaccination or therapeutic treatment, selecting fish embryos post fertilization at the one-cell to eyed egg stage of development, and injecting the yolk sac with carriers associated with an antigen(s) or therapeutic agent(s), may be fully automated.

Disclosed is a novel means which makes it possible to steadily mass-produce knockout individuals even in large animals. The method of the present invention is a method for producing a non-human large mammal or fish (non-human animal) that produces gametes originating in a different individual, and comprises transplanting at least one pluripotent cell derived from a second non-human animal into an embryo derived from a first non-human animal, said embryo being at a cleavage stage and having a genome in which a function of nanos3 gene is inhibited, to prepare a chimeric embryo, and allowing said chimeric embryo to develop into an individual.

Disclosed are non-naturally occurring zebrafish, such as transgenic zebrafish, which comprise a mutation in the rhodopsin (rho) gene. Also disclosed are methods of identifying compounds useful in treating retinal-specific defects and disorders, such as degeneration. Further disclosed are methods of identifying mutations in the rhodopsin gene that can cause retinal-specific defects.

A fish farm having a land-based fish tank (1a-e) and a water supply system (100) with a supply line (2C,D) and a discharge line (2A,B) fluidly connected to a water reservoir (3); a fluid intermediate storage (5,6, 56); a circulation conduit (9a-f) fluidly connected to the fish tank (1a-e); a valve arrangement (10a-p) fluidly connected to the supply line (2C,D), the discharge line (2A,B), the fluid intermediate storage (5,6, 56) and the circulation conduit (9a-f), the valve arrangement (10a-p) having first and second operational configurations for circulation of fluid between the fluid intermediate storage (5,6, 56) and the tank (1a-e) via the circulation conduit (9a-f) and for circulation of fluid between the fluid intermediate storage (5,6, 56) and the reservoir (3) via the supply and discharge lines (2A-D). There is also provided a method for operating a fish farm having at least one land-based fish tank.

Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.

The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.