A composition includes a Euglena biomass lysate having an average particle size of about 2.0 to 500 micrometers and cellular components, including beta-1,3-glucan as at least about 90 percent linear, unbranched beta-1,3-glucan polysaccharide polymers having an average molecular weight of 1.2 to 580 kilodaltons (kDa) and beta-glucan polymer chains having a polymer length of about 7.0 to 3,400 glucose monomers. Residual media remains from a heterotrophic fermentation process that produced a Euglena biomass and the Euglena lysate. Vitamin C is added in an example, and the weight ratio of the beta-1,3-glucan to the added vitamin C is in the ratio of 1:10 to 1:1 and may be in different delivery forms. The composition may include other added immune response inducing components.

A composition includes a Euglena biomass lysate having an average particle size of about 2.0 to 500 micrometers and cellular components, including beta-1,3-glucan as at least about 90 percent linear, unbranched beta-1,3-glucan polysaccharide polymers having an average molecular weight of 1.2 to 580 kilodaltons (kDa) and beta-glucan polymer chains having a polymer length of about 7.0 to 3,400 glucose monomers. Residual media remains from a heterotrophic fermentation process that produced a Euglena biomass and the Euglena lysate. Vitamin C is added in an example, and the weight ratio of the beta-1,3-glucan to the added vitamin C is in the ratio of 1:10 to 1:1 and may be in different delivery forms. The composition may include other added immune response inducing components.

Disclosed is a baked food which comprises a cereal flour, an oil or fat component, astaxanthin and optionally an emulsifying component, has such a structure that the oil or fat component containing the astaxanthin is held in voids in a structure formed by a starch of the cereal flour, and has a porosity of 10 to 70%. Specifically disclosed is a baked food produced from an astaxanthin-containing dough, which can stably hold astaxanthin therein for a long period.

Disclosed is a baked food which comprises a cereal flour, an oil or fat component, astaxanthin and optionally an emulsifying component, has such a structure that the oil or fat component containing the astaxanthin is held in voids in a structure formed by a starch of the cereal flour, and has a porosity of 10 to 70%. Specifically disclosed is a baked food produced from an astaxanthin-containing dough, which can stably hold astaxanthin therein for a long period.

Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.

Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.

Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.

Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.

The present invention relates to a composition comprising a cell wall extract or fraction obtained from protists. The invention also relates to a sugar replacer or low calorie or substantially calorie-free filler or bulking agent comprising a cell wall extract or fraction wherein the cell wall extract or fraction comprises protein, oil and carbohydrate. The invention also provides methods of producing a composition comprising a cell wall extract or fraction and a uses thereof.

The present invention relates to a composition comprising a cell wall extract or fraction obtained from protists. The invention also relates to a sugar replacer or low calorie or substantially calorie-free filler or bulking agent comprising a cell wall extract or fraction wherein the cell wall extract or fraction comprises protein, oil and carbohydrate. The invention also provides methods of producing a composition comprising a cell wall extract or fraction and a uses thereof.