By adding a viable lactic acid bacterium to a fat or oil composition, the present invention has succeeded in producing a lactic acid bacterium-containing fat or oil composition that has a high viability of the lactic acid bacterium and an excellent resistance to acids. By adding lactic acid bacteria powder or a chocolate with a high lactic acid bacterium concentration to a chocolate dough after tempering and before molding, moreover, the present invention has succeeded in producing a lactic acid bacterium-containing chocolate including a viable lactic acid bacterium by a simple producing method. Thus, provided is a lactic acid bacterium-containing chocolate with high palatability that contains a viable lactic acid bacterium.

A food composite, an oral cleaning composite or a medical composite contain at least one lactobacillus strain able to inhibit oral pathogens. The lactobacillus strain is at least one isolated lactobacillus strain selected from a group including Lactobacillus acidophilus F-1 strain; Lactobacillus salivarius subsp. salicinius AP-32 strain; Lactobacillus reuteri GL-104 strain; Lactobacillus paracasei GL-156 strain; Lactobacillus helveticus RE-78 strain; Lactobacillus rhamnosus CT-53 strain; and Lactobacillus paracasei ET-66 strain. The abovementioned lactobacillus strains are able to inhibit oral pathogens and carried by a food composite, an oral cleaning composite or a medical composite.

A food composite, an oral cleaning composite or a medical composite contain at least one lactobacillus strain able to inhibit oral pathogens. The lactobacillus strain is at least one isolated lactobacillus strain selected from a group including Lactobacillus acidophilus F-1 strain; Lactobacillus salivarius subsp. salicinius AP-32 strain; Lactobacillus reuteri GL-104 strain; Lactobacillus paracasei GL-156 strain; Lactobacillus helveticus RE-78 strain; Lactobacillus rhamnosus CT-53 strain; and Lactobacillus paracasei ET-66 strain. The abovementioned lactobacillus strains are able to inhibit oral pathogens and carried by a food composite, an oral cleaning composite or a medical composite.

Embodiments of a method and supplement for preventing and/or treating gastrointestinal distress, including ulcer conditions, in animals are disclosed. The supplement comprises mastic gum and lecithin. The supplement may also comprise B vitamins, one or more prebiotics or probiotics, and one or more minerals as well as, pH buffers and flavoring. A disclosed method of preventing and/or treating gastrointestinal distress comprises orally dosing an animal with the supplement at least one daily.

There is provided a method for recovering spent grain, comprising the steps of: a) adding a batch of mixed solution of a batch of spent grain and a first fermenting composition to a second fermenting composition; b) mixing the added batch of mixed solution with the second fermenting composition, whereby a fermented mixture is obtained; and c) separating the fermented mixture into a liquid product and a solid product; wherein the liquid product and the solid product have a prolonged durability compared to the durability of the batch of spent grain when mixed solution has been added in step a). An advantage of the inventive method is that by-products such as spent grain are efficiently turned into valuable products.

The present application belongs to the technical field of research and development of fermented beverages, and a method for preparing Cordyceps militaris ferment by two-stage fermentation and complex enzymatic hydrolysis is provided. The present application involves preparation of a Cordyceps militaris powder and a Cordyceps militaris slurry, preparation of a Cordyceps militaris fermentation substrate, lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and yeast fermentation, followed by centrifugation, filtration, formulation, sterilization, and filling to obtain a product. By employing lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and secondary fermentation by yeast, the present application can quickly finish a whole fermentation process in 2 to 3 days.

The present invention is a yogurt product that may be consumed by dogs and other mammalian pets. The yogurt product is in an unflavored form comprising as essential constituents: (A) 0% to 88% by weight water; (B) 0.2% to 25% by weight of a no-fat protein component selected from the group consisting of skim milk and cultured skim milk; (C1) 0.001% to 6% by weight sugar component comprising: (C1) 0.001% to 6% polydextrose and (C2) 0.001% to 6% Glycoses and Galactoses; (D) 2% to 8% by weight milk derived solids; (E) 0.2% to 2.5% by weight stabilizers and emulsifiers; and (F) at least one probiotic microorganism. The yogurt product may be refrigerated and served as a mousse, frozen dessert, or as a fermented yogurt drink similar to Ayran or Kefir. A method for producing the yogurt product is also disclosed herein.

Comestible products, for example beverage products, are disclosed containing encapsulated probiotic bacteria having resistance to subjection to at least thermal and acidic conditions. Beverage products include at least one aqueous liquid and capsules comprising a gelled mixture of alginate and denatured protein, and probiotic bacteria entrapped within the gelled mixture. The average particle size of the capsules is optionally less than 1000 microns (m) in diameter, such as less than 500 m in diameter. Methods are provided for making such encapsulated probiotics by providing a mixture comprising sodium alginate, denatured protein and active probiotic cells, and combining the mixture with a divalent cation to initiate cold gelation of the sodium alginate and denatured protein to form a second mixture. The second mixture is passed through an opening having a diameter of less than 1000 m to form capsules. The weight ratio of protein to alginate is from 1:1 to 9:1.

Comestible products, for example beverage products, are disclosed containing encapsulated probiotic bacteria having resistance to subjection to at least thermal and acidic conditions. Beverage products include at least one aqueous liquid and capsules comprising a gelled mixture of alginate and denatured protein, and probiotic bacteria entrapped within the gelled mixture. The average particle size of the capsules is optionally less than 1000 microns (m) in diameter, such as less than 500 m in diameter. Methods are provided for making such encapsulated probiotics by providing a mixture comprising sodium alginate, denatured protein and active probiotic cells, and combining the mixture with a divalent cation to initiate cold gelation of the sodium alginate and denatured protein to form a second mixture. The second mixture is passed through an opening having a diameter of less than 1000 m to form capsules. The weight ratio of protein to alginate is from 1:1 to 9:1.

A process of producing beverage includes washing and drying cactus fruit and pitaya; juicing the cactus fruit to obtain juice of the cactus fruit and juicing the pitaya to obtain juice of the pitaya respectively; filtering the juice of the cactus fruit and the juice of the pitaya respectively; mixing the filtered juice of the cactus fruit and the filtered juice of the pitaya with water to obtain a solution; pouring yeast and gram-positive bacteria in the solution wherein the yeast is saccharomyces boulardii and the gram-positive bacteria includes lactobacillus acidophilus and bifidobacterium longum; and fermenting the solution until beverage is produced wherein fermentation temperature is between 26 C. and 35 C. and fermentation time lasts for 3 to 10 days.