The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells.

The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells.

A smoking article may include a cigarette incorporated within an electrically powered aerosol generating device that acts as a holder for that cigarette. The smoking article possesses at least one form of tobacco. The smoking article also possesses a mouth-end piece that is used by the smoker to inhale components of tobacco that are generated by the action of heat upon components of the cigarette. A representative smoking article possesses an outer housing incorporating a source of electrical power (e.g., a battery), a sensing mechanism for powering the device at least during periods of draw, and a heating device (e.g., at least one electrical resistance heating element) for forming a thermally generated aerosol that incorporates components of tobacco. During use, the cigarette is positioned within the device, and after use, the used cigarette is removed from the device and replaced with another cigarette.

A smoking article may include a cigarette incorporated within an electrically powered aerosol generating device that acts as a holder for that cigarette. The smoking article possesses at least one form of tobacco. The smoking article also possesses a mouth-end piece that is used by the smoker to inhale components of tobacco that are generated by the action of heat upon components of the cigarette. A representative smoking article possesses an outer housing incorporating a source of electrical power (e.g., a battery), a sensing mechanism for powering the device at least during periods of draw, and a heating device (e.g., at least one electrical resistance heating element) for forming a thermally generated aerosol that incorporates components of tobacco. During use, the cigarette is positioned within the device, and after use, the used cigarette is removed from the device and replaced with another cigarette.

Methods of providing an infused tobacco composition including infusing a starting tobacco composition with volatilized ...components formed by heating a volatile component source, thereby retaining at least some volatile components in the starting tobacco composition. Also infused tobacco compositions, as well as aerosol delivery systems and components for use in aerosol delivery systems, including the infused tobacco compositions.

Methods of providing an infused tobacco composition including infusing a starting tobacco composition with volatilized ...components formed by heating a volatile component source, thereby retaining at least some volatile components in the starting tobacco composition. Also infused tobacco compositions, as well as aerosol delivery systems and components for use in aerosol delivery systems, including the infused tobacco compositions.

A method of processing tobacco fines into a non-continuous tobacco material, the method including providing a pre-sized tobacco stem material that has a Dp90 particle size of less than 3 mm and a Dp50 particle size of less than 2 mm. The method also includes combining the pre-sized tobacco stem material with tobacco fines to provide a tobacco initial material, and processing the initial material by setting the initial material to a predefined increased moisture content, subjecting the initial material to an increase in temperature and subjecting the initial material an increased pressure in order to bind the tobacco fines to the tobacco stem material. There is also provided a non-continuous tobacco material produced by the method, a component for a delivery system including non-continuous tobacco material produced by the method, a product and a smoking article including the component.

A method of processing tobacco fines into a non-continuous tobacco material, the method including providing a pre-sized tobacco stem material that has a Dp90 particle size of less than 3 mm and a Dp50 particle size of less than 2 mm. The method also includes combining the pre-sized tobacco stem material with tobacco fines to provide a tobacco initial material, and processing the initial material by setting the initial material to a predefined increased moisture content, subjecting the initial material to an increase in temperature and subjecting the initial material an increased pressure in order to bind the tobacco fines to the tobacco stem material. There is also provided a non-continuous tobacco material produced by the method, a component for a delivery system including non-continuous tobacco material produced by the method, a product and a smoking article including the component.

A combustion retarding material including a combustion retarding salt and an amorphous solid material as well as consumables having the combustion retarding material, and non-combustible aerosol provision systems having such consumables. Methods for manufacturing the combustion retarding material and the use of a combustion retarding salt and an amorphous material to retard combustion.

A combustion retarding material including a combustion retarding salt and an amorphous solid material as well as consumables having the combustion retarding material, and non-combustible aerosol provision systems having such consumables. Methods for manufacturing the combustion retarding material and the use of a combustion retarding salt and an amorphous material to retard combustion.