The present disclosure provides tobacco inbred plants TND950 (phph) SRC, Narrow Leaf Madole (phph) SRC, Narrow Leaf Madole SRC, CMS TND950 SRC, CMS Narrow Leaf Madole SRC, and CMS KY171 SRC, and hybrids PD7305 SRC, PD7309 SRC, PD7312 SRC, PD7318 SRC, and PD7319 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

The present invention provides a method for increasing the sucrose ester content of a tobacco plant or tobacco cell culture, the method comprising modifying said tobacco plant or tobacco cell culture by inhibiting the activity or expression of a diterpene synthesis gene. The present invention also provides for the use of a diterpene synthesis gene for increasing the sucrose ester content of a tobacco plant or tobacco cell culture, as well as tobacco cells, tobacco plants, tobacco plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention. awd

The present invention provides a method for increasing the sucrose ester content of a tobacco plant or tobacco cell culture, the method comprising modifying said tobacco plant or tobacco cell culture by inhibiting the activity or expression of a diterpene synthesis gene. The present invention also provides for the use of a diterpene synthesis gene for increasing the sucrose ester content of a tobacco plant or tobacco cell culture, as well as tobacco cells, tobacco plants, tobacco plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention. awd

The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nornicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.

The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nornicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.

The invention discloses a high-yield integrated production method for cigar wrappers. This method builds a four in one production facility system that integrates seedling cultivation, transplanting, drying, and fermentation processes of cigar wrapper tobacco leaves. The system has a light intensity of 75-160 umol/(m.sup.2.Math.s), a temperature of 255 C. and a relative air humidity of 75%10%; when transplanting tobacco seedlings, using a nutrient solution, which is a high iron boron nutrient solution with an iron content of 0.3-0.7 mM calculated as Fe and a boron content of 9.5-13.0 mg/L calculated as boric acid. The invention achieves a cigar shell yield of over 65%, a 40% reduction in production facility investment, and a 20% reduction in labor force by accurately regulating the production environment and the supply of growth factors such as nutrients and light.

The invention discloses a high-yield integrated production method for cigar wrappers. This method builds a four in one production facility system that integrates seedling cultivation, transplanting, drying, and fermentation processes of cigar wrapper tobacco leaves. The system has a light intensity of 75-160 umol/(m.sup.2.Math.s), a temperature of 255 C. and a relative air humidity of 75%10%; when transplanting tobacco seedlings, using a nutrient solution, which is a high iron boron nutrient solution with an iron content of 0.3-0.7 mM calculated as Fe and a boron content of 9.5-13.0 mg/L calculated as boric acid. The invention achieves a cigar shell yield of over 65%, a 40% reduction in production facility investment, and a 20% reduction in labor force by accurately regulating the production environment and the supply of growth factors such as nutrients and light.

The present disclosure provides tobacco Nic1b locus and associated genes (e.g., a group of ERF genes). Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

The present disclosure provides tobacco Nic1b locus and associated genes (e.g., a group of ERF genes). Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

The present disclosure provides the identification of tobacco Nic1 locus. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Also provided are compositions and methods for producing tobacco plants having novel Nic1 mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or nicotine free while maintaining tobacco leaf grade and tobacco product quality.