Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

The present disclosure provides tobacco inbred plants TND950 (phph) SRC, Narrow Leaf Madole (phph) SRC, Narrow Leaf Madole SRC, CMS TND950 SRC, CMS Narrow Leaf Madole SRC, and CMS KY171 SRC, and hybrids PD7305 SRC, PD7309 SRC, PD7312 SRC, PD7318 SRC, and PD7319 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

Various embodiments are directed to consumable products incorporating a matrix of cultured tobacco cells. Any tobacco variety may be utilized for establishing in vitro tobacco cultures, including native tobacco varieties and genetically modified tobacco varieties derived from any tobacco variety. Various embodiments are directed to methods for producing tobacco products incorporating cellular material and/or extracts derived from tobacco cells cultured in vitro.

Various embodiments are directed to consumable products incorporating a matrix of cultured tobacco cells. Any tobacco variety may be utilized for establishing in vitro tobacco cultures, including native tobacco varieties and genetically modified tobacco varieties derived from any tobacco variety. Various embodiments are directed to methods for producing tobacco products incorporating cellular material and/or extracts derived from tobacco cells cultured in vitro.

This disclosure provides a number of sequences involved in nitrogen utilization, methods of using such sequences, tobacco plants carrying modifications to such sequences, tobacco plants transgenic for such sequences, and tobacco products made from such plants.

This disclosure provides a number of sequences involved in nitrogen utilization, methods of using such sequences, tobacco plants carrying modifications to such sequences, tobacco plants transgenic for such sequences, and tobacco products made from such plants.

A method for improving a sensory quality of a cut rolled stem using a Ganoderma lucidum fermentation broth is provided, including: subjecting Ganoderma lucidum to first-stage cultivation and second-stage expanded cultivation to obtain an extracellular enzyme-containing fungal solution, and filtering the extracellular enzyme-containing fungal solution with a plate and frame filter to obtain a clear fermentation broth without obvious solids; applying the fermentation broth to a tobacco stem or a cut rolled stem, and allowing solid-state fermentation to obtain a fermented tobacco stem or cut rolled stem; and subjecting the fermented tobacco stem or cut rolled stem to expansion, inactivation, and excess water removal. After the fermentation, cell wall substances such as lignin and cellulose are degraded and utilized, which reduces the occurrence of undesirable smells such as a woody smell and a pungent odor, and improves a sensory quality of the cut rolled stem.

A method for improving a sensory quality of a cut rolled stem using a Ganoderma lucidum fermentation broth is provided, including: subjecting Ganoderma lucidum to first-stage cultivation and second-stage expanded cultivation to obtain an extracellular enzyme-containing fungal solution, and filtering the extracellular enzyme-containing fungal solution with a plate and frame filter to obtain a clear fermentation broth without obvious solids; applying the fermentation broth to a tobacco stem or a cut rolled stem, and allowing solid-state fermentation to obtain a fermented tobacco stem or cut rolled stem; and subjecting the fermented tobacco stem or cut rolled stem to expansion, inactivation, and excess water removal. After the fermentation, cell wall substances such as lignin and cellulose are degraded and utilized, which reduces the occurrence of undesirable smells such as a woody smell and a pungent odor, and improves a sensory quality of the cut rolled stem.