Methods of forming cellulosic sugars from the stalk or roots of a plant of the Nicotiana species are provided herein, the methods including i) receiving a tobacco material including at least one of a stalk material and a root material of a harvested plant of the Nicotiana species; ii) delignifying the tobacco material to form a tobacco-derived pulp; and iii) hydrolyzing the tobacco-derived pulp to form a hydrolyzed tobacco product including residual solids and a liquid including at least one tobacco-derived cellulosic sugar. A cellulosic sugar derived from hydrolyzed tobacco material including at least one of a tobacco stalk material and a tobacco root material of a harvested plant of the Nicotiana species is also provided herein. Tobacco products incorporating the cellulosic sugars derived from hydrolyzed tobacco material or products derived from the cellulosic sugar are also provided.

This disclosure provides a number of sequences involved in the transport of alkaloids from the root to the leaf in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences, and tobacco products made from such plants.

This disclosure provides a number of sequences involved in the transport of alkaloids from the root to the leaf in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences, and tobacco products made from such plants.

The present invention relates to a method for processing plant leaves, in particular in the context of producing a fermented product, and to a method for producing a fermented product from plucked plant leaves, comprising the steps: optionally withering the plant leaves, optionally rolling the plant leaves, optionally fermenting the plant leaves, and drying the plant leaves. In order to reduce the expenditure in costs, time and energy of conventional methods for processing plant leaves and for producing a fermented product from plucked plant leaves, respectively, it is provided according to the invention that the plant leaves are conditioned by applying an electric field.

The present invention relates to a method for processing plant leaves, in particular in the context of producing a fermented product, and to a method for producing a fermented product from plucked plant leaves, comprising the steps: optionally withering the plant leaves, optionally rolling the plant leaves, optionally fermenting the plant leaves, and drying the plant leaves. In order to reduce the expenditure in costs, time and energy of conventional methods for processing plant leaves and for producing a fermented product from plucked plant leaves, respectively, it is provided according to the invention that the plant leaves are conditioned by applying an electric field.

The present invention provides a method for modulating the alkaloid content of a plant (e.g. a tobacco plant), the method comprising modifying said plant by modulating the activity or expression of at least one Nic1 ERF gene. The present invention also provides for the use of at least one Nic1 ERF gene for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention.

The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nornicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.

The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nornicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.

An integrally-formed heat-not-burn smoking article and a manufacturing method therefor, wherein the smoking article comprises a lip-proximal end and a lip-distal end, and the smoking article is formed by integrally filling a piece of forming paper having a constant thickness, from the lip-distal end to the lip-proximal end, with four or more different unit sections comprising a filter unit, a gel cooling aroma-carrying unit, a hollow special particle unit, a hollow supporting unit, a cavity unit, a blocking sheet unit and a smoking unit. The manufacturing method involves corresponding integral filling, with the manner of the filling comprising positioned filling in with independent units and integral filling in with combined units. The present invention can reduce the temperature of smoke, reduce a burning, hot and spicy feeling caused by the smoke entering the oral cavity, improve the feeling of a smoking experience.

An integrally-formed heat-not-burn smoking article and a manufacturing method therefor, wherein the smoking article comprises a lip-proximal end and a lip-distal end, and the smoking article is formed by integrally filling a piece of forming paper having a constant thickness, from the lip-distal end to the lip-proximal end, with four or more different unit sections comprising a filter unit, a gel cooling aroma-carrying unit, a hollow special particle unit, a hollow supporting unit, a cavity unit, a blocking sheet unit and a smoking unit. The manufacturing method involves corresponding integral filling, with the manner of the filling comprising positioned filling in with independent units and integral filling in with combined units. The present invention can reduce the temperature of smoke, reduce a burning, hot and spicy feeling caused by the smoke entering the oral cavity, improve the feeling of a smoking experience.