A nicotine pod assembly includes a nicotine reservoir to hold nicotine pre-vapor formulation, and a heater configured to vaporize nicotine pre-vapor formulation drawn from the nicotine reservoir. A device body is configured to engage with the nicotine pod assembly, and includes a controller. The controller is configured to cause the device body to detect a fault event at the nicotine electronic vaping device, classify the fault event as one of a plurality of types of fault events, and perform at least one consequent action based on the classification of the fault event.

A saturation sensor measures at least one electrical characteristic of the wick between the heating element and the probe wire at a first time and a second time, wherein the at least one electrical characteristic includes a resistance, a capacitance, or both a resistance and a capacitance. Control circuitry is configured to cause the nicotine e-vaping device to: calculate a refill rate at which the nicotine pre-vapor formulation flows onto the wick based on the at least one electrical characteristic at the first time and the at least one electrical characteristic at the second time; determine that the refill rate is less than a threshold refill rate; and output a low nicotine pre-vapor formulation alert in response to determining that the refill rate is less than the threshold refill rate.

A method of manufacturing a cannabis herbal supplement. Providing a cannabis profile blend, melting any cannabis wet extract present in the profile formulation, adding any cannabis dry extracts present in the profile formulation, mixing the dry and wet extracts into a mixture or a mash, grinding any flower present in the profile formulation, and adding any flower to the mixture or mash. Cold or heated extraction methods may also be used in this process.

A method of manufacturing a cannabis herbal supplement. Providing a cannabis profile blend, melting any cannabis wet extract present in the profile formulation, adding any cannabis dry extracts present in the profile formulation, mixing the dry and wet extracts into a mixture or a mash, grinding any flower present in the profile formulation, and adding any flower to the mixture or mash. Cold or heated extraction methods may also be used in this process.

A method of modifying the content of certain chemical compounds in tobacco materials is provided, the method including treatment of a tobacco plant component with at least one probiotic. For example, the method may modify the asparagine content in tobacco materials, which can result in a modification in acrylamide production when the tobacco material is exposed to elevated temperatures. The type of tobacco plant component treated according to the invention can be a tobacco seed, a tobacco seedling, an immature live plant, a mature live plant, a harvested plant, or a portion thereof. Examples of probiotics include probiotic species of the genera Bifidobacterium, Lactobacillus, Enterococcus, Proionobacterium, Bacillus, Saccharomyces, Streptococcus, and mixtures thereof. Smoking articles and other tobacco products including such probiotic-treated tobacco materials are also provided.

A method of isolating compounds from a tobacco-derived black liquor, including receiving a black liquor from a pulping process of an input material comprising a plant of the Nicotiana species, treating the black liquor with an acid to lower the pH of the black liquor to about 7 or lower in order to produce a precipitate and an acidified black liquor, separating the precipitate and the acidified black liquor, extracting the acidified black liquor with an organic solvent in order to produce an organic layer extract and an aqueous layer extract, and separating the organic layer extract and the aqueous layer extract.

A method of isolating compounds from a tobacco-derived black liquor, including receiving a black liquor from a pulping process of an input material comprising a plant of the Nicotiana species, treating the black liquor with an acid to lower the pH of the black liquor to about 7 or lower in order to produce a precipitate and an acidified black liquor, separating the precipitate and the acidified black liquor, extracting the acidified black liquor with an organic solvent in order to produce an organic layer extract and an aqueous layer extract, and separating the organic layer extract and the aqueous layer extract.

A method of preparing a whitened tobacco material is provided, the method including the steps of (i) extracting a tobacco material with an aqueous solution to give a tobacco pulp and a tobacco extract; (ii) treating the tobacco pulp with at least one of a caustic reagent and an oxidizing agent for a time and at a temperature sufficient to lighten the color of the tobacco pulp to give a whitened tobacco pulp; (iii) clarifying the tobacco extract to remove higher molecular weight components; and (iv) combining the whitened tobacco pulp with a clarified tobacco extract to form a whitened tobacco material. The whitened tobacco material can be isolated and incorporated into a smokeless tobacco product. The invention also provides a smokeless tobacco product incorporating a whitened tobacco material. The smokeless tobacco product may be a snus-type formulation contained within a sealed pouch.

A method of preparing a whitened tobacco material is provided, the method including the steps of (i) extracting a tobacco material with an aqueous solution to give a tobacco pulp and a tobacco extract; (ii) treating the tobacco pulp with at least one of a caustic reagent and an oxidizing agent for a time and at a temperature sufficient to lighten the color of the tobacco pulp to give a whitened tobacco pulp; (iii) clarifying the tobacco extract to remove higher molecular weight components; and (iv) combining the whitened tobacco pulp with a clarified tobacco extract to form a whitened tobacco material. The whitened tobacco material can be isolated and incorporated into a smokeless tobacco product. The invention also provides a smokeless tobacco product incorporating a whitened tobacco material. The smokeless tobacco product may be a snus-type formulation contained within a sealed pouch.

The present disclosure describes methods of obtaining and/or deriving proteins from plants of the Nicotiana species and methods for incorporation of such proteins into various products. For example, a method for obtaining a protein-enriched material from a plant of the Nicotiana species or portion thereof is provided, comprising: extracting one or more proteins from the plant material into a solvent to form a liquid protein-containing extract; separating a solid extracted plant material from the liquid protein-containing extract; clarifying the liquid protein-containing extract to form a clarified protein-containing extract and a solids fraction; and treating the clarified protein-containing extract so as to provide a protein-enriched material comprising at least about 60% protein by weight.