A consumable for a smoking substitute device contains a liquid aerosol-forming substrate, wherein the substrate comprises an infusion of solid tobacco-derived material in aerosol-former solution, and the infusion is combined with a nicotine formulation. The nicotine formulation may comprise a nicotine salt, particularly nicotine lactate. The substrate is obtained by macerating solid tobacco-derived material in aerosol-former liquid to form an infusion of solid tobacco-derived material and combining the infusion with a nicotine formulation. The tobacco-derived solids are macerated for a time period sufficient for the infusion to undergo a required change in physical characteristics (such as color or viscosity) or a required change in chemical characteristics (such as amount of nicotine release). Tobacco-derived solid may be visible in the substrate as an indicator that it is not a pharmaceutical product.

This document relates to smokeless products containing one or more non-tobacco plant materials. Certain embodiments described in this document relate to smokeless products containing one or more tobacco-alternative plant materials and having properties similar to tobacco-containing oral products.

This document relates to smokeless products containing one or more non-tobacco plant materials. Certain embodiments described in this document relate to smokeless products containing one or more tobacco-alternative plant materials and having properties similar to tobacco-containing oral products.

The present invention relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) or a precursor of a TSNA in tobacco comprising expressing a deregulated cation efflux protein in a tobacco plant, or plant part thereof, or plant cell.

A method of preparing a whitened tobacco material for use in a smokeless tobacco product is provided, including: (i) extracting a tobacco material with an extraction solution to provide a tobacco solids material and a tobacco extract; (ii) cooking the tobacco solids material in an alkaline sulfite cooking liquor including sulfite ions and having a pH of greater than 7 to form a tobacco pulp; (iii) bleaching the tobacco pulp with a bleaching solution to provide a bleached tobacco material; and (iv) drying the bleached tobacco material to provide the whitened tobacco material.

A method of preparing a whitened tobacco material for use in a smokeless tobacco product is provided, including: (i) extracting a tobacco material with an extraction solution to provide a tobacco solids material and a tobacco extract; (ii) cooking the tobacco solids material in an alkaline sulfite cooking liquor including sulfite ions and having a pH of greater than 7 to form a tobacco pulp; (iii) bleaching the tobacco pulp with a bleaching solution to provide a bleached tobacco material; and (iv) drying the bleached tobacco material to provide the whitened tobacco material.

Ribulose-1,5-bisphosphate oxygenase (RuBisCO) protein fibers and a method of producing them are disclosed herein. The method of producing one or more RuBisCO protein fibers including obtaining RuBisCO, for example from tobacco, combining the RuBisCO with one or more plasticizers, heating the combination of the RuBisCO and the one or more plasticizers up to about 140 degrees C., filtering the heated combination through an about 20 μm filter, and passing the filtered combination through an orifice to produce one or more RuBisCO protein fibers.

Ribulose-1,5-bisphosphate oxygenase (RuBisCO) protein fibers and a method of producing them are disclosed herein. The method of producing one or more RuBisCO protein fibers including obtaining RuBisCO, for example from tobacco, combining the RuBisCO with one or more plasticizers, heating the combination of the RuBisCO and the one or more plasticizers up to about 140 degrees C., filtering the heated combination through an about 20 μm filter, and passing the filtered combination through an orifice to produce one or more RuBisCO protein fibers.

The present invention relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) precursor in tobacco, the method comprising modulating the activity or expression of at least one gene encoding a Walls are thin 1-related (WAT1-related) protein.

An aerosolizable formulation comprising (i) water in an amount of at least 20 wt.% based on the aerosolizable formulation; (ii) nicotine; and (iii) an acid which is at least partially water soluble; wherein at least 5 wt% of the nicotine present in the formulation is in protonated form; and wherein the formulation has a pH of from 4 to 6. A process for forming an aerosol comprising the aerosolizable formulation, the aerosolizable formulation contained in a container, an electronic aerosol provision system having an aerosolizer for aerosolizing the aerosolizable formulation for inhalation by a user, a process for improving sensory properties of an aerosolized nicotine formulation, and a process for improving the storage stability of an aerosolized nicotine formulation.