A sparger assembly (700) for a bioprocessing system includes a base plate (710) and at least one aeration manifold (712, 714) removably connected to tire base plate. Each aeration manifold includes at least one inlet for receiving a gas and a plurality of gas outlet openings for delivering tire gas to a fluid within the bioprocessing system. An impeller assembly (740) fora bioprocessing system includes a hub and at least one blade (742) operatively connected to the hub. The at least one blade includes a first portion connected to the hub and extending generally vertically, and a second portion extending at an upward angle from tire first portion.

Aspects of the invention describe a tube diffuser design and associated saddle design that allow the two elements to be easily and quickly attached to each other in the field for the purpose of aerating wastewater. The tube diffuser is cylindrical and includes a a gas distribution section spanning across an interior diameter of the tube diffuser. The saddle includes a pair of engagement arms that are able to couple to this gas distribution section of the tube diffuser. Once the tube diffuser is attached to the saddle and the assembly is submerged in wastewater, the saddle may be used to transport air from an air distribution pipe to the tube diffuser. The air may then be made to leave the tube diffuser as a plume of air bubbles.