A macrosparger (300) for a benchtop bioreactor (370) comprises a tubing (310) and a base (330). The base comprises a plurality of sparging elements (320) in gaseous communication with the tubing and an interior of a benchtop bioreactor. Each sparging element comprises a cylindrical body having an internal compartment formed by a top surface and a bottom surface, the top surface and bottom surface connected by a sidewall, wherein the top surface and bottom surface are substantially oval-shaped. The benchtop bioreactor may be a perfusion bioreactor. A related system comprising the macrosparger and benchtop bioreactor may be used for performing a continuous cell culture.

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface. The bag has a bottom end wall that includes a first sparger and a second sparger. The first sparger and the second sparger each have a flexible first sheet overlying a flexible second sheet, the first sheet and the second sheet being secured together so as to form a sparging area bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the sparging area being gas permeable so that gas can pass from the sparging area, through the first sheet, and into the chamber.

A liquid mixing system includes a support housing at least partially bounding a compartment. A mount is secured to the support housing. A drive motor assembly is configured to engage a drive shaft for moving the drive shaft within the compartment of the support housing. A four bar linkage system extends between the mount and the drive motor assembly, the four bar linkage system being movable between a first position wherein the drive motor assembly is disposed at a first elevation and a second position wherein the drive motor assembly is disposed at a second elevation that is different from the first elevation.

A liquid mixing system includes a support housing at least partially bounding a compartment. A mount is secured to the support housing. A drive motor assembly is configured to engage a drive shaft for moving the drive shaft within the compartment of the support housing. A four bar linkage system extends between the mount and the drive motor assembly, the four bar linkage system being movable between a first position wherein the drive motor assembly is disposed at a first elevation and a second position wherein the drive motor assembly is disposed at a second elevation that is different from the first elevation.

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a bottom end wall being comprised of a first sheet overlying a second sheet, the first sheet and the second sheet of the bottom end wall being welded together so as to form a first sparging area and a separated second sparging area that are both bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the first and second sparging areas being gas permeable.

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a top end wall, a bottom end wall, and an encircling sidewall extending therebetween, the bottom end wall including a first polymeric sheet overlying a second polymeric sheet. A first weld line welds the first polymeric sheet to the second polymeric sheet, the first weld line bounding a first sparging area formed between the first polymeric sheet and the second polymeric sheet. A plurality of first perforations are formed through a portion of the first polymeric sheet that overlies the first sparging area so that gas can pass from the first sparging area, through the first perforations and into the chamber of the bag.

A method of treating effluent seawater generated in the removal of sulfur dioxide from a process gas by contacting the process gas containing sulfur dioxide with seawater, using inclined aeration and mixed auto recovery is provided. Additionally, an inclined aeration and mixed auto recovery seawater oxidation basin system for treating effluent seawater generated in the removal of sulfur dioxide from a process gas by contacting the process gas containing sulfur dioxide with seawater is provided.

A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.

An elongate diffuser is disclosed comprising a diffuser body and a membrane attached to the diffuser body. The membrane is connected to the diffuser body so that introduction of gas at a working pressure into the diffuser displaces part of the membrane from contact with the diffuser body to provide an elongate sealed compartment between the membrane and a surface of the diffuser body. The compartment has a first lateral side interface region where the membrane contacts the diffuser body, a laterally intermediate region where the membrane is spaced apart from the diffuser body and a second lateral side interface region where the membrane contacts the diffuser body. The diffuser body surface which bounds the compartment comprises a recessed portion which is recessed away from the membrane between the first second lateral side interface regions.

An aeration element for gasification or aeration of liquids includes an essentially flattened, rigid support element having a substantially oval cross-section and corrugated outer surfaces, the corrugated outer surfaces including ridges defining grooves therebetween; a threaded opening for receiving a cooperating fitting for connection to an air supply; and a flexible membrane of elastomeric material disposed around the support element. An assembly bracket includes upper and portions that can be cooperatingly attached to one another about the aeration element for securing the aeration element to a floor of a tank or pool. The assembly bracket includes through-going passages for enabling water to circulate around the bracket and reduce buoyancy of the assembly. Two aeration elements of shorter lengths can be positioned adjacent two one another, secured within assembly brackets, and connected at adjacent ends, so that one of the aeration elements functions as a conduit to the other aeration element when air is supplied into one of the aeration elements.