A multi-layered flexible sparger (100) that has a bottom film layer (102), a middle film layer (106), and a top film layer (110); a first inner mesh (104) disposed between the bottom film layer and the middle film layer; a second inner mesh (114) disposed between the middle film layer and the top film layer; and a port capable of delivering a gas to the multi-layered flexible sparger disposed between the top film layer and the bottom film layer, wherein the middle film layer comprises drill holes and the top film layer comprises drill holes.

The invention relates to a ventilation element for the introduction of a gas into a liquid, having at least one gas port, having at least one carrier plate and having at least one elastically deformable diaphragm which is connected to the at least one gas port and/or to the at least one carrier plate. A space that can be formed between the at least one diaphragm and the at least one carrier plate is connected in terms of flow to the gas port. The at least one carrier plate has a multiplicity of gas outlet openings, and at least one diaphragm is composed at least in sections of a material with a lower density than water, or is equipped with at least one float body which has a lower density than water.

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface. The bag has a bottom end wall that includes a first sparger and a second sparger. The first sparger and the second sparger each have a flexible first sheet overlying a flexible second sheet, the first sheet and the second sheet being secured together so as to form a sparging area bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the sparging area being gas permeable so that gas can pass from the sparging area, through the first sheet, and into the chamber.

A screw conveyor for mixing a gas into a liquid and a mixing apparatus including the screw conveyor are disclosed. The screw conveyor includes a helical element having a screw core having a longitudinal axis, a primary spiral including a plurality of primary coils and a secondary spiral supported on the primary spiral and including a plurality of secondary coils. The primary spiral and secondary spiral include a plurality of primary coils and second coils in succession. The secondary spiral is slidably rotatable on the primary spiral. The screw conveyor includes at least one deflector spanning each pair of consecutive primary and secondary coils, wherein the longitudinal axis of each deflector is parallel to the longitudinal axis of the screw core, the deflectors are simultaneously rotatable while maintaining their longitudinal axis parallel to the longitudinal axis of the screw core.

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a bottom end wall being comprised of a first sheet overlying a second sheet, the first sheet and the second sheet of the bottom end wall being welded together so as to form a first sparging area and a separated second sparging area that are both bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the first and second sparging areas being gas permeable.

A container system includes a collapsible bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A sparger is disposed within the chamber of the bag, the sparger bounding a compartment and having an inside edge that encircles an opening passing through the sparger. At least a portion of the sparger is gas permeable. A tubular member is coupled to the sparger and is in communication with the compartment. A shaft passes into the chamber of the bag and through the opening of the sparger. A mixing element is secured to the shaft and is disposed within the chamber of the bag.

A diffuser assembly includes a flexible diffuser membrane and a diffuser body. The flexible diffuser membrane defines a perimeter edge bead along four edges. The diffuser body defines an underlying body portion underlying the flexible diffuser membrane, and a perimeter edge frame covering a covered portion of the perimeter edge bead. The edge frame defines an inside surface that conforms to an outside shape of the covered portion of the perimeter edge bead. The perimeter edge frame is integral to the underlying body portion.

A macrosparger (300) for a benchtop bioreactor (370) comprises a tubing (310) and a base (330). The base comprises a plurality of sparging elements (320) in gaseous communication with the tubing and an interior of a benchtop bioreactor. Each sparging element comprises a cylindrical body having an internal compartment formed by a top surface and a bottom surface, the top surface and bottom surface connected by a sidewall, wherein the top surface and bottom surface are substantially oval-shaped. The benchtop bioreactor may be a perfusion bioreactor. A related system comprising the macrosparger and benchtop bioreactor may be used for performing a continuous cell culture.

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface. The bag has a bottom end wall that includes a first sparger and a second sparger. The first sparger and the second sparger each have a flexible first sheet overlying a flexible second sheet, the first sheet and the second sheet being secured together so as to form a sparging area bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the sparging area being gas permeable so that gas can pass from the sparging area, through the first sheet, and into the chamber.

Disclosed herein is an aeration hose capable of diffusing bubbles of air within a body of water, comprising a) a hose portion derived from a composition, comprising a polyvinyl chloride resin; a first rubber component; a second rubber component; at least one copolymer; at least one low temperature plasticizer; at least one filler comprising antimony trioxide; at least one heat stabilizer; at least one internal lubricant; at least one antioxidant; and at least one biofouling agent, wherein the hose portion comprises an outer hose portion, an inner hose portion and a plurality of hose apertures capable of receiving and diffusing pressurized air, wherein the hose is flexible and has no memory, wherein the hose apertures are provided therethrough the inner hose portion and the outer hose portion and proportionally spaced about the outer hose portion along a length of the hose portion.