A composition for remediation of soil and groundwater containing halogenated compounds. The remediation composition includes an elemental iron-based composition, which may include activated carbon capable of absorbing the halogenated compounds with numerous pores impregnated with elemental iron. The remediation composition further includes a first bioremediation material including a blend of one-to-many organisms capable of degrading the halogenated compounds. The remediation composition includes an organic compound or polymeric substance and a second bioremediation material including a blend of one-to-many organisms capable of degrading the organic compound or polymeric substance over time (e.g., 20 to 365 or more days to provide a time release substrate-creating material or platform) into smaller molecules or compounds used by the organisms in the first bioremediation material while degrading the halogenated compounds. The organic compound may be a complex carbohydrate such as food grade starch, chitin, or other complex carbohydrate such as one with low water solubility.

The present application discloses a Bacillus sp. producing a bioflocculant and a biosurfactant. The microbial classification of the Bacillus sp. is named Bacillus sp. SS15, which has been preserved in China Center for Type Culture Collection on Mar. 29, 2021, and its preservation number is CCTCC M2021295; the 16S rRNA sequence of SS15 is shown as SEQ ID NO.1. Bacillus sp. SS15 obtained by the present application has the function of simultaneously producing a bioflocculant and a biosurfactant. The bioflocculant and biosurfactant produced by Bacillus sp. SS15 of the present application not only have high activity, but also show strong tolerance in the ranges of pH (2-12), temperature (4° C.-100° C.) and salinity (0-100 g/L). Meanwhile, they can be applied to the remediation of a fracturing flowback liquid and can effectively promote the removal of chroma, suspended solids, COD, n-alkanes and polycyclic aromatic hydrocarbons at the same time.

The present application discloses a Bacillus sp. producing a bioflocculant and a biosurfactant. The microbial classification of the Bacillus sp. is named Bacillus sp. SS15, which has been preserved in China Center for Type Culture Collection on Mar. 29, 2021, and its preservation number is CCTCC M2021295; the 16S rRNA sequence of SS15 is shown as SEQ ID NO.1. Bacillus sp. SS15 obtained by the present application has the function of simultaneously producing a bioflocculant and a biosurfactant. The bioflocculant and biosurfactant produced by Bacillus sp. SS15 of the present application not only have high activity, but also show strong tolerance in the ranges of pH (2-12), temperature (4° C.-100° C.) and salinity (0-100 g/L). Meanwhile, they can be applied to the remediation of a fracturing flowback liquid and can effectively promote the removal of chroma, suspended solids, COD, n-alkanes and polycyclic aromatic hydrocarbons at the same time.

A method for advanced nitrogen and phosphorus removal of domestic sewage based on DEAMOX in AOAO process with sludge double-reflux is disclosed. The method comprises allowing domestic sewage and returned sludge of the secondary sedimentation tank (3) to enter the anaerobic zone (2.1) of the AOAO reactor (2), firstly performing partial denitrification by the denitrifying bacteria, reducing nitrate-nitrogen in the returned sludge to nitrite-nitrogen, then converting ammonia-nitrogen and nitrite-nitrogen into nitrogen by anammox bacteria, and phosphate accumulating bacteria and denitrifying phosphate accumulating organisms performing anaerobic phosphate release and storing internal carbon source; then allowing part of the mixed liquid to enter the intermediate aerobic zone (2.2) of the AOAO bioreactor (2) to carry out phosphate uptake and nitrification reaction, allowing another part of the mixed liquid to enter the anoxic zone (2.3) of the AOAO bioreactor (2), at same time allowing all the mixed liquid of the intermediate aerobic zone (2.2) and part of returned sludge of the secondary sedimentation tank (3) to enter the anoxic zone (2.3), using the internal carbon source stored in the anaerobic compartment and the internal carbon source in the returned sludge to carry out partial denitrification, anammox, denitrifying dephosphatation, and then allowing the mixed liquid to enter the post aerobic zone (2.4) and subsequently enter the secondary sedimentation tank (3) for mud-water separation. An apparatus for advanced nitrogen and phosphorus removal of domestic sewage based on DEAMOX in AOAO process with sludge double-reflux is also disclosed.

A composition for stimulating the production and excretion of a lignolytic enzyme in a microorganism for degrading harmful substances and/or in the manufacturing of easily degradable ester containing plastics or articles made of ester containing plastic. The composition mainly includes tributyrin, triolein, fish oil, 16-hydroxyhexadecanoic acid, n-aliphatic primary fatty alcohols, polycaprolactone, aliphatic polyesters, linolenic acid, linoleic acid, alpha linolenic acid, plant polyesters, cutin, cutin derivatives, cutin monomers, omega hydroxy acids, 16-hydroxy palmitic acid, 9,16-dihydroxypalmitic acid, 10,16-dihydroxypalmitic acid, C18-hydroxy oleic acid, 9,10-epoxy-18-hydroxy stearic acid, 9,10,18-trihydroxystearate, suberin, cork, fruit skins, vegetable skins, and their constituents and derivatives, hydroxy fatty acids, 16-hydroxy palmitic acid, 18-hydroxy stearic acid, juniperic acid, hexadecanol, linseed oil, perilla oil, amides, acetamide and N-acetyl amide, zinc, zinc salts, butyrate, acetate, lactate, manganese peroxidase, and carbamide peroxide.

A method for treating wastewater comprising subjecting a sulfate containing wastewater to Fe(III) iron dosing in an anaerobic bioreactor containing one or more of an iron reducing bacteria and one or more of a sulfate reducing bacteria, and one or more of a fermentative bacteria, and adjusting a dosage of the Fe(III) iron in the anaerobic bioreactor to achieve a Fe/Sulfate molar ratio that is equal to or greater than 0.50, and removing an effluent from the anaerobic bioreactor that is a treated wastewater. A wastewater treatment system is provided having a wastewater reservoir, a ferric iron solution reservoir, an anaerobic bioreactor, and an effluent reservoir.

The highway sponge-type composite side ditch carbon neutralization system of the present invention includes three units, i.e., a silt pre-sedimentation channel, a filtering and oil-absorbing channel and an ecologic purification channel which are connected horizontally and successively. These three units work together to jointly complete the low-impact development functions of runoff collection, guide and drainage, purification and utilization, carbon emission is reduced by adopting various technical measures, the carbon sink effect is improved, and the full-life-cycle carbon neutralization effect from raw material production, construction to operation can be realized.

A system for removing undesirable compounds from contaminated air includes a biofilter having an alkaline material introduction system and a fuzzy-logic based controller. A contaminant, such as hydrogen sulfide, is removed from contaminated air by passing the contaminated air through the biofilter.

A filter system and filter suitable for water treatment includes a filter having a housing with a water inlet and an outlet, the housing defining a filtration chamber therein. The filtration chamber has filter media therein having a plurality of at least partially porous filtration beads and a pump for pumping water to the inlet. Such a filtration system enables high nutrient removal thus minimising the food source for algae.

A filter system and filter suitable for water treatment includes a filter having a housing with a water inlet and an outlet, the housing defining a filtration chamber therein. The filtration chamber has filter media therein having a plurality of at least partially porous filtration beads and a pump for pumping water to the inlet. Such a filtration system enables high nutrient removal thus minimising the food source for algae.