A method for reducing toxic mercury in wastewater effluent comprises the steps of: identifying a system into which wastewater effluent is introduced, the wastewater effluent including organic compounds and organomercurial compounds; producing a treatment composition comprising a solution including a surfactant, digestive microbes suspended in the solution, and mercury-transformative microbes suspended in the solution; and providing the treatment composition into the system containing the wastewater effluent, such that the digestive microbes degrade the organic compounds in the wastewater effluent, and the mercury-transformative microbes reduce the organomercurial compounds in the wastewater effluent into nontoxic volatile elemental mercury. In certain systems for reducing toxic mercury in wastewater effluent, a biological capture medium is positioned within a vessel and configured to provide a capture point for microbes to adhere to and create biofilms. One such system is a dental evacuation system in which wastewater effluent is introduced into the system via an aspirator.

Surfactants based on a newly discovered class of compounds include a hydrophobic lipid oligomer covalently linked to a peptide or peptide-like chain and a carbohydrate moiety, and a serine-leucinol dipeptide linked to the lipid oligomer. Such surfactants can be used to create an oil-in-water or water-in-oil emulsion by mixing together a polar component; a non-polar component; and the surfactant. Biosurfactants of the newly discovered class can be made by isolating and culturing a microorganism which produces the biosurfactant, and then isolating the biosurfactant from the culture. A microorganism can be engineered to produce biosurfactant of this newly discovered class by expressing a set of heterologous genes involved in the biosynthesis of the biosurfactant in the microorganism.

Surfactants based on a newly discovered class of compounds include a hydrophobic lipid oligomer covalently linked to a peptide or peptide-like chain and a carbohydrate moiety, and a serine-leucinol dipeptide linked to the lipid oligomer. Such surfactants can be used to create an oil-in-water or water-in-oil emulsion by mixing together a polar component; a non-polar component; and the surfactant. Biosurfactants of the newly discovered class can be made by isolating and culturing a microorganism which produces the biosurfactant, and then isolating the biosurfactant from the culture. A microorganism can be engineered to produce biosurfactant of this newly discovered class by expressing a set of heterologous genes involved in the biosynthesis of the biosurfactant in the microorganism.

A method for improving the quality of pond water used in aquaculture applications by adding to the pond water active bacteria that are preferably germinated from spores on site using a nutrient-germinant composition and an incubation method for increased spore germination efficiency, in combination with a nitrification enhancement agent such as calcium carbonate or calcified seaweed, and an optional reaction surface area modifier such as calcified seaweed or plastic or metal particles or fragments. The nutrient-germinant composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for germination. The incubation method comprises heating a nutrient germinant composition and bacteria spores, to a temperature range of 35° C. to 60° C. for around 2 to 60 minutes to produce an incubated bacteria solution that is discharged to the aquaculture application.

A Septic Aid system includes at least tablet containing dissolvable septic treatment so constituted as to supply a septic system with replacement bacteria and/or enzymes. The at least one tablet is configured to slowly release the septic treatment into toilet water. The at least one tablet may also have a dissolvable color indicator configured to run out at approximately the same time as the dissolvable septic treatment is depleted. An attachment may be provided in the form of an adhesive disc, suction cup, or hangar. An applicator may be used to apply the at least one tablet to an interior surface of the toilet.

A spore germination composition and method to produce a bioaugmentation solution that is added to an anaerobic digester or partially aerobic digester to increase biogas production. A nutrient-germinant composition comprises L-amino acids a phosphate buffer, an industrial preservative, and an optional source of potassium. The composition and spores of one or more Bacillus species are heated to a preferred elevated temperature range of 35° C. to 60° C. for an incubation period of around 20 to 60 minutes to form a bioaugmentation solution that is dispensed to the digester, preferably to the hydrolysis stage of the digester. A dose of bioaugmentation solution is added to the digester around once per day in an amount to provide at least 1000 CFU per mL of the full volume capacity of the digester, which can increase methane production by around 5 to 10% over operation of the digester without the bioaugmentation solution.

Described herein are methods for eliminating biuret from an environment such as a body of water, through treatment with an effective amount of a hypochlorite. Additionally described herein are methods of eliminating biuret resulting from enzymatic degradation of CYA in an aqueous solution.

Described herein are methods for eliminating biuret from an environment such as a body of water, through treatment with an effective amount of a hypochlorite. Additionally described herein are methods of eliminating biuret resulting from enzymatic degradation of CYA in an aqueous solution.

The invention relates to a method for aerobic and anaerobic cultivation of microorganisms. The invention also relates to a method for producing a preparation for cleaning contaminated liquids and surfaces. Likewise, the invention relates to a method for cleaning contaminated liquids and surfaces.

The present invention relates to a process and plant for treating feed water containing nitrate. The process includes, sorbing nitrate from the feed water onto an ion exchange resin to form a loaded resin and produce a treated water stream depleted in nitrate, regenerating the loaded resin so that the resin can be reused and produce a brine stream high in nitrate; and converting nitrate in the brine stream into molecular nitrogen gas with the assistance of a bioactive agent.