The present invention relates to a mainstream deammonification process for removing ammonium from wastewater that suppresses NOB growth and produces a sludge having good settling characteristics, the process comprising: clarifying the wastewater stream in a primary clarifier (12) and producing a primary effluent; directing a first portion of the primary effluent to a biological treatment reactor (14) and removing carbon to produce treated wastewater; directing treated wastewater into an integrated fixed film activated sludge (IFAS) deammonification reactor (16) integrating nitritation and anammox processes and that is provided with intermittent aeration; directing a second portion of the primary effluent to the IFAS deammonification reactor (16) by-passing the biological treatment reactor (14), and injecting this second portion only during periods of air off and refraining from injecting during periods of air on, directing the IFAS deammonification reactor (16) effluent to a secondary clarifier (18) and producing a secondary effluent and a clarifier underflow, and recycling at least a portion of the underflow to the IFAS deammonification reactor (16).

A method for controlled biomass densification in a biological treatment of a raw influent, includes a step of subjecting the raw influent to a biological treatment of free suspended biomass, thereby producing a biomass comprising activated sludge; a step of separation and/or clarification of the activated sludge, thereby producing an effluent and a RAS; a step of extracting at least part of the RAS and/or part of the activated sludge as a first source of a WAS; a step of external density-based selection of at least part of the RAS and/or part of the activated sludge, thereby generating an overflow intended to be extracted as a second source of WAS, and an underflow comprising dense biomass aggregates; a step of producing and/or sustaining dense biomass aggregates, such as aerobic granular sludge or biofilm, by a dense biomass aggregates generating process, with at least part of the raw influent; a step of subjecting the dense biomass aggregates to the biological treatment; a step of subjecting the dense biomass aggregates of the underflow to the biological treatment and/or to the dense biomass aggregates generating process; thereby obtaining a densified biomass.

Methods of treating animal organic material are disclosed herein. The methods include diluting the animal organic material to produce an organic material slurry, anaerobically digesting the organic material slurry to produce a biogas and a digestate, separating the digestate to produce a digestate solids and a filtrate, removing ammonia from the filtrate, removing organic contaminants and divalent anions from the filtrate, concentrating the filtrate to produce a retentate and a permeate, combining the digestate solids and the retentate to produce a solids product, and returning the permeate to dilute the animal organic material. Systems for treatment of animal organic material are also disclosed herein. The systems include a dilution tank, an anaerobic digester, a first solids-liquid separation subsystem, an ammonia-reducing column, a second solids-liquid separation subsystem, a production water storage tank, and a solids product tank.

High levels of polyhydroxyalkanoates (PHA) can be produced from wastewater comprising Readily Biodegradable COD (RBCOD) using activated sludge comprising microorganisms capable of accumulating PHA by contacting the wastewater with the activated sludge in the presence of dissolved oxygen during a first period of time, to obtain PHA-loaded activated sludge, and then supplying elements essential for growth such as nitrogen and phosphorus and allowing up-take of these elements and limited growth during a second period of time, the supplied amount of at least of one of said essential elements compared to the amount of RBCOD supplied in step a) limiting the growth to an extent that not all PHA is used for growth, to obtain grown activated sludge; and removing or harvesting part of the PHA-loaded activated sludge and/or part of the grown activated sludge, so that the total average retention time of the sludge is less than 72 h.

A process for constructing a soil-based rhizosphere flow-through system to break down contaminants in contaminated water. The process includes the steps of: providing plants planted in soil in a test bioreactor, the plants providing a rhizosphere; exposing the rhizosphere to the contaminated water, extracting microorganisms from the rhizosphere following their exposure to the contaminated water: preparing a microbial suspension from the extract; subjecting the microbial suspension to growth conditions to increase the concentration of the microorganism, thereby preparing a soil conditioner; adding the soil conditioner to soil in a contained area having a water flow inlet and outlet; and planting a plurality of plants in the soil, the plants being of the same species as the plants of the test bioreactor.

A wastewater treatment system including a contact tank, a dissolved air flotation unit, a fermentation unit, and a biological treatment unit is disclosed. A method of retrofitting a wastewater treatment system by arranging the wastewater treatment system such that floated biosolids are fermented in an anerobic environment and fluidly connecting the biological treatment unit to receive at least a portion of the fermented solids is also disclosed. The method optionally includes providing a fermentation unit and fluidly connecting the fermentation unit to a biological treatment unit. A method of treating wastewater including combining the wastewater with activated sludge, floating biosolids from the activated wastewater, fermenting the floated biosolids, and biologically treating the effluent with the fermented solids is also disclosed. A method of facilitating delivery of soluble organic carbon to a biological treatment unit is also disclosed.

The microorganism preparation feeding method of the invention employs an automatic microorganism preparation feeding apparatus which includes a cold storage apparatus for refrigeration-storing a seed microorganism belonging to the aerobic microorganism group including at least one species of aerobic microorganisms capable of decomposing oil and fat contained in oil/fat-including wastewater and a growth tank for growing the seed microorganism so as to produce the microorganism preparation, wherein the seed microorganism belonging to the aerobic microorganism group is maintained in a live state by means of the cold storage apparatus, the seed microorganism is periodically grown by means of the growth tank so as to produce a predetermined microorganism preparation, and the produced predetermined microorganism preparation is fed to the oil/fat-including wastewater. The method includes refrigeration-storing, as the seed microorganism, a microorganism whose population density is 1×10.sup.7 CFU/mL to 5×10.sup.9 CFU/mL in the cold storage apparatus; growing, as a source material, the seed microorganism of a predetermined volume by means of the growth tank so as to produce the predetermined microorganism preparation whose volume is 50 to 500 times the predetermined volume of the seed microorganism and whose population density is 1×10.sup.7 CFU/mL to 2×10.sup.10 CFU/mL; and feeding the produced microorganism preparation to the oil/fat-including wastewater.

A continuous flow wastewater treatment system is disclosed. The system can include a main processing circuit comprising an anoxic entry zone and an aerobic exit zone. The system can include a granule-producing sidestream incubator. The sidestream incubator can include an inlet that receives a first portion of return activated sludge (RAS) processed by the aerobic exit zone, a sidestream anoxic zone, and a sidestream anaerobic zone that cooperate to process the first portion of RAS. The sidestream incubator can include an outlet that delivers the processed first portion of RAS to the anoxic entry zone. The sidestream incubator can include a RAS bypass pathway that bypasses the granule-producing sidestream incubator to deliver a second portion of RAS processed by the aerobic exit zone to the anoxic entry zone. In some embodiments, the RAS and/or other fermentate can be step-fed into the sidestream incubator.

A system and method for generating an incubated bacteria solution by heating a nutrient germinant composition and bacteria, including at least one species in spore form, to a preferred temperature a range of 35-50° C. for 2-60 minutes using exothermic chemical reaction heat. An incubated bacteria solution is preferably generated at or near a point-of-use in an aquaculture, agriculture, wastewater, or environmental remediation application. The nutrient-germinant composition comprises L-amino acids, optionally D-glucose and/or D-fructose, a buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for incubation. A first chemical contained in a pouch is activated by contact with a second chemical, water, or air in a flameless heater to initiate exothermic reaction to provide incubation heat. A potable, single-use incubation bag is configured to hold the flameless heater and a container of nutrient germinant composition and spores.

A wastewater treatment apparatus includes a treatment mechanism that treats a wastewater containing an organic chromaticity component with an enzyme produced by Bacillus proteolyticus.