The invention relates to a method for obtaining a mineral substance from a base comprising mineral matter, the method comprising obtaining the base comprising a predetermined quantity of the mineral matter synthesised by a living structure or a portion of the latter, characterised in that obtaining the base comprises providing the living structure and providing at least one lactic acid microorganism suitable for symbiosis with the living structure for the synthesis of the mineral matter of the mineral substance.

The invention also relates to a mineralising composition comprising a living structure, a lactic acid microorganism, a nutritive substance; the mineral substance used in the method; and the use of a combination of a living structure and a lactic acid microorganism in symbiosis with each other as a mineralising agent in a self-regenerating material.

The invention relates to a method for obtaining a mineral substance from a base comprising mineral matter, the method comprising obtaining the base comprising a predetermined quantity of the mineral matter synthesised by a living structure or a portion of the latter, characterised in that obtaining the base comprises providing the living structure and providing at least one lactic acid microorganism suitable for symbiosis with the living structure for the synthesis of the mineral matter of the mineral substance.

The invention also relates to a mineralising composition comprising a living structure, a lactic acid microorganism, a nutritive substance; the mineral substance used in the method; and the use of a combination of a living structure and a lactic acid microorganism in symbiosis with each other as a mineralising agent in a self-regenerating material.

Methods for producing construction material utilizing loose pieces of aggregate (30), enzyme producing bacteria, an amount of urea and an amount of calcium ions. A first solution is prepared which includes urease which is formed by enzyme producing bacteria. A second solution is prepared which includes urea and calcium ions. The first and second solutions are added to the loose aggregate (30). The calcium ions contribute to the formation of calcium carbonate wherein the calcium carbonate fills and bonds between at least some of the gaps between the loose pieces of aggregate forming a solid construction material (92).

Methods for producing construction material utilizing loose pieces of aggregate (30), enzyme producing bacteria, an amount of urea and an amount of calcium ions. A first solution is prepared which includes urease which is formed by enzyme producing bacteria. A second solution is prepared which includes urea and calcium ions. The first and second solutions are added to the loose aggregate (30). The calcium ions contribute to the formation of calcium carbonate wherein the calcium carbonate fills and bonds between at least some of the gaps between the loose pieces of aggregate forming a solid construction material (92).

Described are a method and composition which use microorganisms for downhole applications, in particular for the maintenance or restoration of the integrity of a cement sheath in a wellbore. The method of cementing a wellbore comprises preparing a cement composition comprising a pumpable slurry of cement, water and a healing agent that comprises one or more microorganisms, delivering the cement composition into the wellbore and allowing the cement composition to set. The method of restoring the integrity of a set cement composition in a wellbore comprises preparing a pumpable slurry that comprises water, a healing agent that comprises one or more microorganisms and optionally cement, pumping the slurry into the wellbore in and around the set cement composition, and allowing the one or more microorganisms to grow and replicate to thereby restore the integrity of the set cement composition. Compositions for carrying out the methods are also described.

Described are a method and composition which use microorganisms for downhole applications, in particular for the maintenance or restoration of the integrity of a cement sheath in a wellbore. The method of cementing a wellbore comprises preparing a cement composition comprising a pumpable slurry of cement, water and a healing agent that comprises one or more microorganisms, delivering the cement composition into the wellbore and allowing the cement composition to set. The method of restoring the integrity of a set cement composition in a wellbore comprises preparing a pumpable slurry that comprises water, a healing agent that comprises one or more microorganisms and optionally cement, pumping the slurry into the wellbore in and around the set cement composition, and allowing the one or more microorganisms to grow and replicate to thereby restore the integrity of the set cement composition. Compositions for carrying out the methods are also described.

A soil solidification material based on solid waste and bioenzyme, and a preparation method thereof are disclosed. The soil solidification material is composed of the following components in parts by weight: recycled aggregate 22-35 parts, steel slag 20-30 parts, high-calcium fly ash 16-24 parts, the bioenzyme 5-15 parts, an inorganic adsorbent 10-18 parts, an organic adsorbent 8-20 parts, industrial waste gypsum 25-35 parts, an activator 20-30 parts, sodium citrate 1-3 parts, and slaked lime 0.02-0.2 parts. The present disclosure adopts the recycled aggregate, the steel slag, the industrial waste gypsum and the high-calcium fly ash as the main components of the soil solidification material to reduce the cost. The soil solidification material of the present disclosure prepared by optimizing the proportion is capable of significantly improving the engineering properties of the soil or the mixed contaminated soil, and has significant economic and environmental benefits.

A soil solidification material based on solid waste and bioenzyme, and a preparation method thereof are disclosed. The soil solidification material is composed of the following components in parts by weight: recycled aggregate 22-35 parts, steel slag 20-30 parts, high-calcium fly ash 16-24 parts, the bioenzyme 5-15 parts, an inorganic adsorbent 10-18 parts, an organic adsorbent 8-20 parts, industrial waste gypsum 25-35 parts, an activator 20-30 parts, sodium citrate 1-3 parts, and slaked lime 0.02-0.2 parts. The present disclosure adopts the recycled aggregate, the steel slag, the industrial waste gypsum and the high-calcium fly ash as the main components of the soil solidification material to reduce the cost. The soil solidification material of the present disclosure prepared by optimizing the proportion is capable of significantly improving the engineering properties of the soil or the mixed contaminated soil, and has significant economic and environmental benefits.

The invention is directed to kits, compositions, tools and methods comprising a cyclic industrial process to form biocement. In particular, the invention is directed to materials and methods for decomposing calcium carbonate into calcium oxide and carbon dioxide at an elevated temperature, reacting calcium oxide with ammonium chloride to form calcium chloride, water, and ammonia gas; and reacting ammonia gas and carbon dioxide at high pressure to form urea and water, which are then utilized to form biocement. This cyclic process can be achieved by combining industrial processes with the resulting product as biocement. The process may involve retention of calcium carbonate currently utilized in the manufacture of Portland Cement.

A method of enhancing carbonate precipitation in a downhole environment comprises introducing into the downhole environment a treatment composition comprising: a carbonate producing agent comprising a microbe, an enzyme, or a combination comprising at least one of the foregoing, and a substrate comprising N-oxyurea, semicarbazide, N,N-dioxyurea, or a combination comprising at least one of the foregoing. An organic feedstock and a geobacter can also be used to treating a wellbore or a subterranean formation. Encapsulated carbonate producing agent such as encapsulated bacterial spores are used to form self-healing cemented structure in a downhole environment.