The present invention provides methods for mineral precipitation and/or cementation of permeable or fractured non-porous materials using isolated urease.

The present disclosure discloses a method for improving the performance of concrete aggregates, comprising the following steps: (1) adding composite microbial powders, recycled aggregates and water into a stirring pot in a certain ratio to be continuously and uniformly stirred; (2) placing the recycled aggregate obtained in step (1) and another microbial powder in a vacuum device so as to fill the microbial powder into the cracks of the recycled aggregate in a negative-pressure environment; and (3) spraying a calcium source solution to the surface of the recycled aggregate obtained in step (2) at an interval of 5-6 hours for repeating 3-5 times to maintain the wet surface of the recycled aggregate.

A method for producing photocatalytic mortar includes providing a mortar-producing material including a fine aggregate and cement, a reactant mixture including a zinc source and urea, and a microorganism-containing mixture including water and a urease-producing microorganism, subjecting the microorganism-containing mixture and the reactant mixture to microbial induced precipitation in the mortar-producing material, subjecting zinc carbonate crystal-containing mortar produced to curing for the same to undergo hydration, and subjecting cured mortar to hydrothermal synthesis, so that zinc carbonate crystals therein are converted to nano zinc oxide crystals.

Design and construction method for improving an expansive soil embankment using phosphogypsum and microbes, including the following steps: (1) placing Bacillus pasteurii into a culture medium to prepare a microbial solution, and mixing urea and calcium chloride with water to prepare a cementing fluid; (2) mixing and stirring a mixture, the microbial solution and water, adding the cementing solution well, and mixing the cementing fluid with water to prepare an improved mixture; and (3) leveling and compacting original ground, laying geomembranes, the improved mixture, and geogrids, laying a last layer of geomembrane on the top surface of the embankment after pavement of the embankment, and paving a roadbed. The design and construction method can meet construction requirements of highway embankment projects and roadbed projects of first-grade and other grades of roads, and consume solid waste phosphogypsum.

Design and construction method for improving an expansive soil embankment using phosphogypsum and microbes, including the following steps: (1) placing Bacillus pasteurii into a culture medium to prepare a microbial solution, and mixing urea and calcium chloride with water to prepare a cementing fluid; (2) mixing and stirring a mixture, the microbial solution and water, adding the cementing solution well, and mixing the cementing fluid with water to prepare an improved mixture; and (3) leveling and compacting original ground, laying geomembranes, the improved mixture, and geogrids, laying a last layer of geomembrane on the top surface of the embankment after pavement of the embankment, and paving a roadbed. The design and construction method can meet construction requirements of highway embankment projects and roadbed projects of first-grade and other grades of roads, and consume solid waste phosphogypsum.

The present disclosure provides a method of biocementation comprising contacting a granular, cohesionless soil with a solution, wherein the solution comprises urea, urease, a source of calcium ions, and a source of non-urease proteins, wherein the urea, urease, source of calcium ions, and source of non-urease proteins are provided in effective amounts suitable to cause crystallization of calcium carbonate.

This present invention relates to compositions to improve concrete using biochemical-producing microbes and/or byproducts synthesized by the microbes. The invention also relates to methods for enhancing the performance of the concrete with said microbial strains and/or their byproducts.

This present invention relates to compositions to improve concrete using biochemical-producing microbes and/or byproducts synthesized by the microbes. The invention also relates to methods for enhancing the performance of the concrete with said microbial strains and/or their byproducts.

A composition contains at least one microorganism which can form a phosphate or carbonate precipitate in an alkaline medium, and at least one calcium source. The composition has at least one silicon compound having at least one Si-atom, at least one C-atom, and at least one H-atom. The composition can be used in a method for producing a construction product.

A composition contains at least one microorganism which can form a phosphate or carbonate precipitate in an alkaline medium, and at least one calcium source. The composition has at least one silicon compound having at least one Si-atom, at least one C-atom, and at least one H-atom. The composition can be used in a method for producing a construction product.