The invention is directed to compositions and methods for the manufacture of pigmented solids structures for which can be used for construction and/or decoration. Manufacturing comprises fixing one or more pigments to an aggregate material such as crushed rock, stone or sand. The pigmented aggregate is incubated with urease or urease producing microorganisms, an amount of a nitrogen source such as urea, and an amount of calcium source such as calcium chloride forming calcite bridges between particles of aggregate. The resulting solid has a hardness and colorfastness for most any construction material. Using selected aggregate and pigment, the process also provides for the manufacture of simulated-stone materials such as clay or granite bricks or blocks, marble counter-tops, and more. The invention is also directed to composition containing microorganisms and pigment as kits that can be added to most any aggregate materials.

The invention is directed to compositions and methods for the manufacture of pigmented solids structures for which can be used for construction and/or decoration. Manufacturing comprises fixing one or more pigments to an aggregate material such as crushed rock, stone or sand. The pigmented aggregate is incubated with urease or urease producing microorganisms, an amount of a nitrogen source such as urea, and an amount of calcium source such as calcium chloride forming calcite bridges between particles of aggregate. The resulting solid has a hardness and colorfastness for most any construction material. Using selected aggregate and pigment, the process also provides for the manufacture of simulated-stone materials such as clay or granite bricks or blocks, marble counter-tops, and more. The invention is also directed to composition containing microorganisms and pigment as kits that can be added to most any aggregate materials.

The present invention provides methods for mineral precipitation and/or cementation of permeable or fractured non-porous materials using isolated urease.

The present invention provides methods for mineral precipitation and/or cementation of permeable or fractured non-porous materials using isolated urease.

The invention is directed to compositions and methods for the manufacture of pigmented solids structures for which can be used for construction and/or decoration. Manufacturing comprises fixing one or more pigments to an aggregate material such as crushed rock, stone or sand. The pigmented aggregate is incubated with urease or urease producing microorganisms, an amount of a nitrogen source such as urea, and an amount of calcium source such as calcium chloride forming calcite bridges between particles of aggregate. The resulting solid has a hardness and colorfastness for most any construction material. Using selected aggregate and pigment, the process also provides for the manufacture of simulated-stone materials such as clay or granite bricks or blocks, marble counter-tops, and more. The invention is also directed to composition containing microorganisms and pigment as kits that can be added to most any aggregate materials.

The invention is directed to compositions and methods for the manufacture of pigmented solids structures for which can be used for construction and/or decoration. Manufacturing comprises fixing one or more pigments to an aggregate material such as crushed rock, stone or sand. The pigmented aggregate is incubated with urease or urease producing microorganisms, an amount of a nitrogen source such as urea, and an amount of calcium source such as calcium chloride forming calcite bridges between particles of aggregate. The resulting solid has a hardness and colorfastness for most any construction material. Using selected aggregate and pigment, the process also provides for the manufacture of simulated-stone materials such as clay or granite bricks or blocks, marble counter-tops, and more. The invention is also directed to composition containing microorganisms and pigment as kits that can be added to most any aggregate materials.

A soil stabilization method of forming an admixture of at least one hydrogel precursor and urease enzyme, and contacting at least a portion of the admixture with soil, and forming a hydrogel network in-situ within at least a portion of the soil, wherein at least a portion of the hydrogel network includes in-situ precipitated calcium carbonate. In some embodiments, at least a portion of the hydrogel network is formed in-situ by polymerizing the at least one hydrogel precursor in the presence of the urease enzyme.

A construction method for reinforcing a loess embankment by combining microbial mineralization with phosphogypsum. The method includes: (1) placing Bacillus pasteurii in a culture medium to prepare a microbial solution, and mixing urea, calcium chloride and water to prepare a cementing solution; (2) mixing a mixture, the microbial solution and water well, and adding the cementing solution and water to prepare an improving mixture; and (3) leveling and compacting an original ground; laying a geomembrane, the improving mixture and a geogrid; laying a last geomembrane on the top of the embankment after the embankment is laid, and then laying a roadbed. The method meets the improvement and construction requirements of loess embankments of high-grade highways, and reduces the stock of phosphogypsum, thereby reducing the occupation of cultivated land. The microbial improvement of the phosphogypsum achieves the immobilization of harmful elements, thereby reducing environmental pollution.

A construction method for reinforcing a loess embankment by combining microbial mineralization with phosphogypsum. The method includes: (1) placing Bacillus pasteurii in a culture medium to prepare a microbial solution, and mixing urea, calcium chloride and water to prepare a cementing solution; (2) mixing a mixture, the microbial solution and water well, and adding the cementing solution and water to prepare an improving mixture; and (3) leveling and compacting an original ground; laying a geomembrane, the improving mixture and a geogrid; laying a last geomembrane on the top of the embankment after the embankment is laid, and then laying a roadbed. The method meets the improvement and construction requirements of loess embankments of high-grade highways, and reduces the stock of phosphogypsum, thereby reducing the occupation of cultivated land. The microbial improvement of the phosphogypsum achieves the immobilization of harmful elements, thereby reducing environmental pollution.

The invention is directed to compositions, tools and methods for the manufacture of construction materials, masonry, solid structures and compositions to facilitate dust control. More particularly, the invention is directed to the manufacture of bricks, masonry and other solid structures using small amount of aggregate material that is pre-loaded with spores and/or vegetative bacterial cells.