The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plants life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

An improved solvent system for the formulation and application of N-alkyl thiophosphoric triamide urease inhibitors. These formulations provide safety and performance benefits relative to existing alternatives and enable storage, transport and subsequent coating or blending with urea based or organic based fertilizers. These formulations are comprised primarily of environmentally friendly aprotic and protic solvents (particularly dimethyl sulfoxide and alcohols/polyols) to stabilize the urease inhibitor.

An improved solvent system for the formulation and application of N-alkyl thiophosphoric triamide urease inhibitors. These formulations provide safety and performance benefits relative to existing alternatives and enable storage, transport and subsequent coating or blending with urea based or organic based fertilizers. These formulations are comprised primarily of environmentally friendly aprotic and protic solvents (particularly dimethyl sulfoxide and alcohols/polyols) to stabilize the urease inhibitor.

The invention relates to a solid, particulate, urea-based blend composition comprising a urea-based compound in particulate form, a component comprising an ammonium source in particulate form, a urease inhibitor of the type phosphoric triamide, an alkaline or alkaline-forming inorganic or organic compound that is able to interact with the component comprising an ammonium source in particulate form, selected from the group consisting of metal oxides, metal carbamates, metal hydroxides, metal acetates and any mixtures thereof, or from the group of organic bases consisting of ammonia, amines, amides, adenines, amidines, guanidines, anilines, carbamates, thiazoles, triazoles, pyridines; imidazoles, benzimidazoles, histidines, phosphazenes, and any mixture thereof, wherein the urea-based blend composition further comprises a cation source, different from the alkaline or alkaline-forming inorganic or organic compound, comprising a cation selected from the group consisting of Fe.sup.2+, Fe.sup.3+, Mn.sup.2+, Zn.sup.2+, Cu.sup.+, Cu.sup.2+, Ni.sup.2+, Ag.sup.+, Pt.sup.2+, Ru.sup.2+, Co.sup.3+ and Cr.sup.3+.

The invention relates to a solid, particulate, urea-based blend composition comprising a urea-based compound in particulate form, a component comprising an ammonium source in particulate form, a urease inhibitor of the type phosphoric triamide, an alkaline or alkaline-forming inorganic or organic compound that is able to interact with the component comprising an ammonium source in particulate form, selected from the group consisting of metal oxides, metal carbamates, metal hydroxides, metal acetates and any mixtures thereof, or from the group of organic bases consisting of ammonia, amines, amides, adenines, amidines, guanidines, anilines, carbamates, thiazoles, triazoles, pyridines; imidazoles, benzimidazoles, histidines, phosphazenes, and any mixture thereof, wherein the urea-based blend composition further comprises a cation source, different from the alkaline or alkaline-forming inorganic or organic compound, comprising a cation selected from the group consisting of Fe.sup.2+, Fe.sup.3+, Mn.sup.2+, Zn.sup.2+, Cu.sup.+, Cu.sup.2+, Ni.sup.2+, Ag.sup.+, Pt.sup.2+, Ru.sup.2+, Co.sup.3+ and Cr.sup.3+.

This present disclosure relates to a solid, particulate, urea ammonium sulphate-based composition, further comprising one or more alkaline or alkaline-forming inorganic or organic compounds that are able to interact with ammonium sulphate, a urease inhibitor of the type phosphoric triamide, and a cation source comprising a cation selected from the group consisting of Fe.sup.2+, Fe.sup.3+, Mn.sup.2+, Zn.sup.2+, Cu.sup.+, Cu.sup.2+, Ni.sup.2+, Ag.sup.+, Pt.sup.2+, Ru.sup.2+, Co.sup.3+ and Cr.sup.3+. The invention further relates to a method for the manufacture of a solid, particulate urea ammonium sulphate-based composition comprising urea, ammonium sulphate, a urease inhibitor of the type phosphoric triamide, in particular N-(n-butyl) thiophosphoric triamide (nBTPT), one or more alkaline or alkaline-forming inorganic or organic compounds and a cation source. The product is in particular suitable for use as a fertilizer.

This present disclosure relates to a solid, particulate, urea ammonium sulphate-based composition, further comprising one or more alkaline or alkaline-forming inorganic or organic compounds that are able to interact with ammonium sulphate, a urease inhibitor of the type phosphoric triamide, and a cation source comprising a cation selected from the group consisting of Fe.sup.2+, Fe.sup.3+, Mn.sup.2+, Zn.sup.2+, Cu.sup.+, Cu.sup.2+, Ni.sup.2+, Ag.sup.+, Pt.sup.2+, Ru.sup.2+, Co.sup.3+ and Cr.sup.3+. The invention further relates to a method for the manufacture of a solid, particulate urea ammonium sulphate-based composition comprising urea, ammonium sulphate, a urease inhibitor of the type phosphoric triamide, in particular N-(n-butyl) thiophosphoric triamide (nBTPT), one or more alkaline or alkaline-forming inorganic or organic compounds and a cation source. The product is in particular suitable for use as a fertilizer.

A method for manufacturing a urea-formaldehyde slow-release nitrogen fertilizer includes steps of: mixing and heating urea and formaldehyde solution with a predetermined molar ratio of urea to formaldehyde; adjusting a PH value of hydroxymethylation reaction of urea and formaldehyde solution to 7.5-10.5; heating the hydroxymethylation reaction of urea and formaldehyde solution to the initial reaction temperature of 50° C.; adding a catalyst to start the hydroxymethylation reaction of urea and formaldehyde solution, and conducting hydroxymethylation reaction intermittently or continuously; heating cold urea-formaldehyde solution using the reaction heat of hydroxymethylation; adjusting the pH value of the methylenation reaction of urea and formaldehyde solution to 3.5-5.0; adding a catalyst; completing the methylenation reaction of urea and formaldehyde solution within 1 to 10 minutes; and performing spraying granulation of slurry after the methylenation reaction of urea and formaldehyde solution in the granulator to obtain a urea-formaldehyde slow-release nitrogen fertilizer.

A fluidized bed granulator for production of urea-containing or nitrate-containing granules may include a granulator interior having granulator interior walls with a first granulator side wall, a second granulator side wall, a granulator front wall that extends transversely to the granulator side walls, and a granulator back wall that likewise extends transversely at the opposite end of the granulator interior from the granulator front wall, a horizontal perforated plate that bounds the granulator interior in a downward direction, a seed entry opening, and a granule exit opening that is disposed at a distance in front of the granulator back wall. A process for producing urea-containing or nitrate-containing granules may utilize the fluidized bed granulator.

A nitrogen stabilizing composition is provided. The composition includes 6% to 18% N-(n-butyl) thiophosphoric triamide (NBPT), 1% to 6% 3,4-dimethyl pyrazole phosphate (DMPP), and a solvent, wherein the ratio of NBPT to DMPP is between about 5.5:1 and 6.5:1.