A two-part curable urethane adhesive composition of the present technology contains a base agent containing a urethane prepolymer, and a curing agent containing a compound having two or more active hydrogen groups in each molecule. The curing agent contains at least one type of terpene compound selected from the group consisting of monoterpenes, hydrogenated monoterpenes, modified monoterpenes formed by modifying the monoterpenes or the hydrogenated monoterpenes with hydroxy groups, and oligomers having from 2 to 6 repeating units derived from the monoterpenes or the modified monoterpenes.

In one aspect, the invention provides a method for synthesizing a fatty olefin derivative. The method includes: a) contacting an olefin according to Formula I

##STR00001##

with a metathesis reaction partner according to Formula IIb

##STR00002##

in the presence of a metathesis catalyst under conditions sufficient to form a metathesis product according to Formula IIIb:

##STR00003##

and b) converting the metathesis product to the fatty olefin derivative. Each R.sup.1 is independently selected from H, C.sub.1-18 alkyl, and C.sub.2-18 alkenyl; R.sup.2b is C.sub.1-8 alkyl; subscript y is an integer ranging from 0 to 17; and subscript z is an integer ranging from 0 to 17. In certain embodiments, the metathesis catalyst is a tungsten catalyst or a molybdenum catalyst. In various embodiments, the fatty olefin derivative is a pheromone. Pheromone compositions and methods of using them are also described.

In one aspect, the invention provides a method for synthesizing a fatty olefin derivative. The method includes: a) contacting an olefin according to Formula I

##STR00001##

with a metathesis reaction partner according to Formula IIb

##STR00002##

in the presence of a metathesis catalyst under conditions sufficient to form a metathesis product according to Formula IIIb:

##STR00003##

and b) converting the metathesis product to the fatty olefin derivative. Each R.sup.1 is independently selected from H, C.sub.1-18 alkyl, and C.sub.2-18 alkenyl; R.sup.2b is C.sub.1-8 alkyl; subscript y is an integer ranging from 0 to 17; and subscript z is an integer ranging from 0 to 17. In certain embodiments, the metathesis catalyst is a tungsten catalyst or a molybdenum catalyst. In various embodiments, the fatty olefin derivative is a pheromone. Pheromone compositions and methods of using them are also described.

A method for extraction of desirable compounds from cannabis provides for high-cannabinoid full spectrum (HCFSE) extractions, using CO.sub.2 at low temperature to extract the compounds in an extraction vessel. The CO.sub.2 with extracted compounds is transferred to a heated vessel which converts the CO.sub.2 to a gas, allowing the desired compounds to drop out and be removed.

A method for extraction of desirable compounds from cannabis provides for high-cannabinoid full spectrum (HCFSE) extractions, using CO.sub.2 at low temperature to extract the compounds in an extraction vessel. The CO.sub.2 with extracted compounds is transferred to a heated vessel which converts the CO.sub.2 to a gas, allowing the desired compounds to drop out and be removed.

The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.

The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.

An improved method for preparing squalene from a squalene-containing composition, said method comprising the steps of (a) a purification distillation carried out at a temperature T.sub.1 (b) a denaturing distillation carried out at a temperature T.sub.2; wherein steps (a) and (b) may be performed in either order; T.sub.1 and T.sub.2 are sufficient to cause squalene to boil; T.sub.2>T.sub.1; and T.sub.2>200 C.

An improved method for preparing squalene from a squalene-containing composition, said method comprising the steps of (a) a purification distillation carried out at a temperature T.sub.1 (b) a denaturing distillation carried out at a temperature T.sub.2; wherein steps (a) and (b) may be performed in either order; T.sub.1 and T.sub.2 are sufficient to cause squalene to boil; T.sub.2>T.sub.1; and T.sub.2>200 C.

Methods and compositions for stabilization and subsequent hydrogenation of a microbial-derived immiscible olefin are described. The methods comprise separating immiscible olefin from a mixture comprising an aqueous solution, microbial cells and immiscible olefin thereby forming a crude olefin composition; purifying the crude olefin composition thereby forming a purified olefin composition; and adding a phenolic antioxidant to the purified olefin composition wherein the phenolic antioxidant is a phenol derivative containing an unfused phenyl ring with one or more hydroxyl substituents. The methods further comprise reacting the purified olefin composition with hydrogen in the presence of a hydrogen catalyst such that hydrogen saturates at least one double bond in the olefin. Hydrogenated compositions produced by the methods are further provided.