The present application discloses methods for the efficient preparation of high purity compounds of the Formula I, and their methods of use.

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The present application discloses methods for the efficient preparation of high purity compounds of the Formula I, and their methods of use.

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A method of producing thymoquinone and/or thymohydroquinone in Monarda by growing Monarda with elevated levels of carvacrol and/or thymol in the fresh plant tissue and vigorous growth, harvesting the Monarda, leaving the Monarda to senensce in the presence of oxygen and extracting the thymoquinone and/or thymohydroquinone from the senesced tissue.

A method of producing thymoquinone and/or thymohydroquinone in Monarda by growing Monarda with elevated levels of carvacrol and/or thymol in the fresh plant tissue and vigorous growth, harvesting the Monarda, leaving the Monarda to senensce in the presence of oxygen and extracting the thymoquinone and/or thymohydroquinone from the senesced tissue.

A method of producing thymoquinone and/or thymohydroquinone in Monarda by growing Monarda with elevated levels of carvacrol and/or thymol in the fresh plant tissue and vigorous growth, harvesting the Monarda, leaving the Monarda to senensce in the presence of oxygen and extracting the thymoquinone and/or thymohydroquinone from the senesced tissue.

A method of preparing a Senna obtusifolia seed extract rich in anthraquinones and a galactomannan extract includes the following steps: (1) crushing Senna obtusifolia seeds into a Senna obtusifolia seed powder; (2) extracting the Senna obtusifolia seed powder with 40-85% ethanol, filtering to obtain an extract solution and a residue; (3) concentrating the extract solution under vacuum to obtain a concentrated extract solution, spray-drying the concentrated extract solution to obtain the Senna obtusifolia seed extract; (4) extracting the residue with membrane filtered water, conducting a centrifugation to obtain a supernatant; (5) adding ammonium sulfate and ethanol to the supernatant to form a two-phase aqueous system, collecting a bottom layer of the two-phase aqueous system; and (6) conducting an ultrafiltration of the bottom layer with a cut-off molecular weight of 50 k-200 k to obtain a galactomannan extract solution, drying the galactomannan extract solution under vacuum to obtain the galactomannan extract.

A method of preparing a Senna obtusifolia seed extract rich in anthraquinones and a galactomannan extract includes the following steps: (1) crushing Senna obtusifolia seeds into a Senna obtusifolia seed powder; (2) extracting the Senna obtusifolia seed powder with 40-85% ethanol, filtering to obtain an extract solution and a residue; (3) concentrating the extract solution under vacuum to obtain a concentrated extract solution, spray-drying the concentrated extract solution to obtain the Senna obtusifolia seed extract; (4) extracting the residue with membrane filtered water, conducting a centrifugation to obtain a supernatant; (5) adding ammonium sulfate and ethanol to the supernatant to form a two-phase aqueous system, collecting a bottom layer of the two-phase aqueous system; and (6) conducting an ultrafiltration of the bottom layer with a cut-off molecular weight of 50 k-200 k to obtain a galactomannan extract solution, drying the galactomannan extract solution under vacuum to obtain the galactomannan extract.

The present disclosure relates to a process for preparing a Lawsonia inermis extract which is rich in Lawsone. It also relates to the extract which may be obtained by said process and to a composition comprising thereof. The disclosure also relates to a method for dying fibers, in particular keratin fibers.

The present disclosure relates to a process for preparing a Lawsonia inermis extract which is rich in Lawsone. It also relates to the extract which may be obtained by said process and to a composition comprising thereof. The disclosure also relates to a method for dying fibers, in particular keratin fibers.

A method for preparing 2-alkylanthracene includes the step of separating 2-alkylanthracene from a reaction product of anthracene alkylation reaction. The anthracene alkylation reaction is a reaction of anthracene and an alkylation reagent under an alkylation condition and in the presence of an alkylation reaction solvent and a catalyst. The reaction product of the anthracene alkylation reaction contains anthracene and the product of a series of alkylanthracenes containing 2-alkylanthracene.