A method of purification of crude cis-2-alkenoic acid by thermal separation, comprising wiped-film evaporation or vacuum distillation of the crude cis-2-alkenoic acid, under a temperature-reduced pressure profile whereby isomerization of cis-2-alkenoic acid into trans-2-alkenoic acid is minimized.

A method of purification of crude cis-2-alkenoic acid by thermal separation, comprising wiped-film evaporation or vacuum distillation of the crude cis-2-alkenoic acid, under a temperature-reduced pressure profile whereby isomerization of cis-2-alkenoic acid into trans-2-alkenoic acid is minimized.

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by removing water from the cell fermentation broth or lysed cell composition before demulsification is conducted. Such process has the benefits of reduced demulsification time and reduced salt use. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by the process described herein.

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by removing water from the cell fermentation broth or lysed cell composition before demulsification is conducted. Such process has the benefits of reduced demulsification time and reduced salt use. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by the process described herein.