The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate (2H3M4OP) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M4OP, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (CO.sub.2) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.

The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate (2H3M4OP) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M4OP, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (CO.sub.2) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.

A method for producing highly pure p-toluic acid by a simple solid-liquid separation operation using as a raw material a substance inducible from a biomass resource and suitable for fermentation production by a microorganism is disclosed. The method for producing p-toluic acid includes: a dehydration reaction step of dehydrating Compound(s) (1)-(4), which are substances inducible from biomass resources and suitable for fermentation production by microorganisms; and a solid-liquid separation step of recovering a solid produced by this dehydration reaction by a simple solid-liquid separation operation.

A method for producing highly pure p-toluic acid by a simple solid-liquid separation operation using as a raw material a substance inducible from a biomass resource and suitable for fermentation production by a microorganism is disclosed. The method for producing p-toluic acid includes: a dehydration reaction step of dehydrating Compound(s) (1)-(4), which are substances inducible from biomass resources and suitable for fermentation production by microorganisms; and a solid-liquid separation step of recovering a solid produced by this dehydration reaction by a simple solid-liquid separation operation.

A method for producing highly pure p-toluic acid by a simple solid-liquid separation operation using as a raw material a substance inducible from a biomass resource and suitable for fermentation production by a microorganism is disclosed. The method for producing p-toluic acid includes: a dehydration reaction step of dehydrating Compound(s) (1)-(4), which are substances inducible from biomass resources and suitable for fermentation production by microorganisms; and a solid-liquid separation step of recovering a solid produced by this dehydration reaction by a simple solid-liquid separation operation.

Processes for recovering a purified aromatic carboxylic acid include contacting a crude aromatic carboxylic acid with hydrogen in the presence of a catalyst in a hydrogenation reactor to form a purified aromatic carboxylic acid; crystallizing the purified aromatic carboxylic acid to form a solid/liquid mixture comprising purified aromatic carboxylic acid solids; filtering the solid/liquid mixture in a rotary pressure filter apparatus to remove a liquid filtrate, washing the solid/liquid mixture in the rotary pressure apparatus with a wash fluid to form a washed solid/liquid mixture, and drying the washed solid/liquid mixture in the rotary pressure apparatus with an inert gas to form a filter cake comprising purified aromatic carboxylic acid solids and a wet gas stream; withdrawing the wet gas stream from the rotary pressure filter apparatus while maintaining the wet gas stream at a pressure above ambient; and recovering the purified aromatic carboxylic acid solids from the filter cake.

Processes for recovering a purified aromatic carboxylic acid include contacting a crude aromatic carboxylic acid with hydrogen in the presence of a catalyst in a hydrogenation reactor to form a purified aromatic carboxylic acid; crystallizing the purified aromatic carboxylic acid to form a solid/liquid mixture comprising purified aromatic carboxylic acid solids; filtering the solid/liquid mixture in a rotary pressure filter apparatus to remove a liquid filtrate, washing the solid/liquid mixture in the rotary pressure apparatus with a wash fluid to form a washed solid/liquid mixture, and drying the washed solid/liquid mixture in the rotary pressure apparatus with an inert gas to form a filter cake comprising purified aromatic carboxylic acid solids and a wet gas stream; withdrawing the wet gas stream from the rotary pressure filter apparatus while maintaining the wet gas stream at a pressure above ambient; and recovering the purified aromatic carboxylic acid solids from the filter cake.

A cannabis infused oil is formed by collecting and drying raw cannabis material having a desired cannabinoid profile. Next, the raw dried cannabis material is blended with oil to obtain a cannabis infused oil mixture. Blending is performed without causing the cannabis material to become decarboxylated. Next, the cannabis material is filtered from the cannabis infused oil mixture thereby obtaining a cannabis infused oil. The cannabis infused oil has at least two cannabinoids present in the oil in accordance with the desired cannabinoid profile. Next, the cannabis infused oil is packaged for storage and shipment. The cannabis infused oil is formed without any alcohol, does not have any decarboxylated cannabinoids and is non-psychoactive. In one example, the cannabis infused oil has between 100 milligrams and 2,000 milligrams of cannabinoids per fluid ounce of cannabis infused oil. In another example, before blending with oil, the cannabis material is combined with alcohol.

A cannabis infused oil is formed by collecting and drying raw cannabis material having a desired cannabinoid profile. Next, the raw dried cannabis material is blended with oil to obtain a cannabis infused oil mixture. Blending is performed without causing the cannabis material to become decarboxylated. Next, the cannabis material is filtered from the cannabis infused oil mixture thereby obtaining a cannabis infused oil. The cannabis infused oil has at least two cannabinoids present in the oil in accordance with the desired cannabinoid profile. Next, the cannabis infused oil is packaged for storage and shipment. The cannabis infused oil is formed without any alcohol, does not have any decarboxylated cannabinoids and is non-psychoactive. In one example, the cannabis infused oil has between 100 milligrams and 2,000 milligrams of cannabinoids per fluid ounce of cannabis infused oil. In another example, before blending with oil, the cannabis material is combined with alcohol.

A method for producing highly pure p-toluic acid by a simple solid-liquid separation operation using as a raw material a substance inducible from a biomass resource and suitable for fermentation production by a microorganism is disclosed. The method for producing p-toluic acid includes: a dehydration reaction step of dehydrating Compound(s) (1)-(4), which are substances inducible from biomass resources and suitable for fermentation production by microorganisms; and a solid-liquid separation step of recovering a solid produced by this dehydration reaction by a simple solid-liquid separation operation.