The present invention relates to a complex comprising a transition metal cation (i); a ligand (ii) comprising at least one chelating group, preferably from 1 to 4 chelating groups, more preferred 2 or 3 chelating groups, wherein the chelating group(s) is/are selected from hydroxamate group N(O)C(O)R, catechol ate group, carboxyl ate group, partly or totally protonated forms of these chelating groups and mixtures of these chelating groups and/or their partly or totally protonated forms, wherein R is hydrogen or a C1 to C5 alkyl group; and a magnetic bead (iii); wherein the magnetic bead (iii) and the ligand (ii) are covalently bonded. The invention also relates to the use of the complex for reduction of the content of at least one phosphor-oxy-substance, which preferably comprises a structural element OP(O)(O)O within its structure, in a fluid sample, as well as to a method for decreasing the content of at least one phosphor-oxy-substance, preferably a phospholipid, in a fluid sample, comprising a step of adding the complex. The invention further relates to a supernatant obtained or obtainable from this method and to the use of a supernatant obtained or obtainable from the method for qualitative and/or quantitative determination of at least one analyte in said supernatant. Furthermore, the invention relates to a method for qualitative and/or quantitative determination of at least one analyte in a fluid sample and to a method for determining the kind and/or amount of at least one phosphor-oxy-substance in a fluid sample.

The present invention relates to a complex comprising a transition metal cation (i); a ligand (ii) comprising at least one chelating group, preferably from 1 to 4 chelating groups, more preferred 2 or 3 chelating groups, wherein the chelating group(s) is/are selected from hydroxamate group N(O)C(O)R, catechol ate group, carboxyl ate group, partly or totally protonated forms of these chelating groups and mixtures of these chelating groups and/or their partly or totally protonated forms, wherein R is hydrogen or a C1 to C5 alkyl group; and a magnetic bead (iii); wherein the magnetic bead (iii) and the ligand (ii) are covalently bonded. The invention also relates to the use of the complex for reduction of the content of at least one phosphor-oxy-substance, which preferably comprises a structural element OP(O)(O)O within its structure, in a fluid sample, as well as to a method for decreasing the content of at least one phosphor-oxy-substance, preferably a phospholipid, in a fluid sample, comprising a step of adding the complex. The invention further relates to a supernatant obtained or obtainable from this method and to the use of a supernatant obtained or obtainable from the method for qualitative and/or quantitative determination of at least one analyte in said supernatant. Furthermore, the invention relates to a method for qualitative and/or quantitative determination of at least one analyte in a fluid sample and to a method for determining the kind and/or amount of at least one phosphor-oxy-substance in a fluid sample.

The present disclosure provides a photolabile protecting group compound comprising a thiophene ring, a nitrobenzyl group having a nitro group in an ortho position relative to a benzylic carbon, an alkyne linker connecting the thiophene ring to the nitrobenzyl group, and a hydroxamic acid moiety attached to the benzylic carbon through an oxygen atom, wherein upon irradiation with ultraviolet light, the compound undergoes photolysis to release the hydroxamic acid and generate a fluorescent nitrosoketone byproduct. The thiophene ring is connected to the nitrobenzyl group through a 2-position of the thiophene ring. The nitrobenzyl group has a methyl group attached to the benzylic carbon. The compound has an absorption maximum between 340 nm and 360 nm, and upon photolysis, the fluorescent nitrosoketone byproduct has an emission maximum between 440 nm and 500 nm with a 3-fold to 4-fold increase in fluorescence intensity that is observable with a naked eye.

The present disclosure provides a photolabile protecting group compound comprising a thiophene ring, a nitrobenzyl group having a nitro group in an ortho position relative to a benzylic carbon, an alkyne linker connecting the thiophene ring to the nitrobenzyl group, and a hydroxamic acid moiety attached to the benzylic carbon through an oxygen atom, wherein upon irradiation with ultraviolet light, the compound undergoes photolysis to release the hydroxamic acid and generate a fluorescent nitrosoketone byproduct. The thiophene ring is connected to the nitrobenzyl group through a 2-position of the thiophene ring. The nitrobenzyl group has a methyl group attached to the benzylic carbon. The compound has an absorption maximum between 340 nm and 360 nm, and upon photolysis, the fluorescent nitrosoketone byproduct has an emission maximum between 440 nm and 500 nm with a 3-fold to 4-fold increase in fluorescence intensity that is observable with a naked eye.