The object of the present invention is to provide a process for preparing a 5-alken-1-yne compound efficiently at low costs and a process for preparing (2E,6Z)-2,6-nonadienal by making use of the aforesaid process for preparing the 5-alken-1-yne compound. There is provided a process for preparing a 5-alken-1-yne compound of the following formula (4): YZCR.sup.1CR.sup.2(CH.sub.2).sub.2CCH (4) in which Y in formula (4) represents a hydrogen atom or a hydroxyl group, the process comprising at least steps of: subjecting (i) an alkenylmagnesium halide compound prepared from a haloalkene compound of the following formula (1): YZCR.sup.1CR.sup.2(CH.sub.2).sub.2X.sup.1 (1) and (ii) an alkyne compound of the following formula (2): X.sup.2CCSi(R.sup.3)(R.sup.4)(R.sup.5) (2) to a coupling reaction to form a silane compound of the following formula (3): YZCR.sup.1CR.sup.2(CH.sub.2).sub.2CCSi(R.sup.3)(R.sup.4)(R.sup.5) (3); and subjecting the silane compound (3) to a desilylation reaction to form the 5-alken-1-yne compound (4).

The invention relates to a method for preparing a compound (I) or one of the salts thereof, and the uses of said method, R.sup.1OOCC(X)CHR.sup.2R.sup.3(I), wherein X is selected among O; NR, wherein R is H or a C1-C6 alkyl; and NOR wherein R is H, or a C1-C6 alkyl or an alkylaryl; R.sup.1 is H or a C1-C6 alkyl group; R.sup.2 is H, a C1-C6 alkyl, or an alkylaryl; and R.sup.3 is CH.sub.2SR.sup.4 or CH.sub.2SeR.sup.4, where R.sup.4 is H or a C1-C6 alkyl from a compound (II), or one of the salts thereof, R.sup.1OOCC(X)CHR.sup.2R.sup.5 and R.sup.5 is H or COOR.sup.6, where R.sup.6 is H or a C1-C6 alkyl, said method being carried out in the presence of a compound (III) CH.sub.2(Y)(Z). Wherein Y is H; OR.sup.7, where R.sup.7 is H, a C1-C6 alkyl or an acyl with formula COR.sup.4; SR.sup.4 or SeR.sup.4 where R.sup.4 matches the preceding definition; or 1 NR.sup.8R.sup.9, where R.sup.8 and R.sup.9, identical or different, each or together are a C1-C6 alkyl, or an alkylaryl; Z, identical or different to Y, is OR.sup.10 where R.sup.10 is H, a C1-C6 alkyl or COR.sup.4; a cyclic or acyclic N(COR.sup.4)(COR.sup.4) group; or NR.sup.11R.sup.12, where R.sup.11 and R.sup.12, identical or different, each or together are a C1-C6 alkyl or an alkylaryl; wherein Y and Z together are O; said method involving an intermediate product (IV) R.sup.1OOCC(X)CHR.sup.2CH.sub.2Z.

The invention relates to a method for preparing a compound (I) or one of the salts thereof, and the uses of said method, R.sup.1OOCC(X)CHR.sup.2R.sup.3(I), wherein X is selected among O; NR, wherein R is H or a C1-C6 alkyl; and NOR wherein R is H, or a C1-C6 alkyl or an alkylaryl; R.sup.1 is H or a C1-C6 alkyl group; R.sup.2 is H, a C1-C6 alkyl, or an alkylaryl; and R.sup.3 is CH.sub.2SR.sup.4 or CH.sub.2SeR.sup.4, where R.sup.4 is H or a C1-C6 alkyl from a compound (II), or one of the salts thereof, R.sup.1OOCC(X)CHR.sup.2R.sup.5 and R.sup.5 is H or COOR.sup.6, where R.sup.6 is H or a C1-C6 alkyl, said method being carried out in the presence of a compound (III) CH.sub.2(Y)(Z). Wherein Y is H; OR.sup.7, where R.sup.7 is H, a C1-C6 alkyl or an acyl with formula COR.sup.4; SR.sup.4 or SeR.sup.4 where R.sup.4 matches the preceding definition; or 1 NR.sup.8R.sup.9, where R.sup.8 and R.sup.9, identical or different, each or together are a C1-C6 alkyl, or an alkylaryl; Z, identical or different to Y, is OR.sup.10 where R.sup.10 is H, a C1-C6 alkyl or COR.sup.4; a cyclic or acyclic N(COR.sup.4)(COR.sup.4) group; or NR.sup.11R.sup.12, where R.sup.11 and R.sup.12, identical or different, each or together are a C1-C6 alkyl or an alkylaryl; wherein Y and Z together are O; said method involving an intermediate product (IV) R.sup.1OOCC(X)CHR.sup.2CH.sub.2Z.

The present invention relates to a composition for clearing a biotissue and a method for clearing a biotissue using thereof.

The present invention relates to a composition for clearing a biotissue and a method for clearing a biotissue using thereof.

Provided is an improved process for the preparation N-[4-[[4-(diethyl amino) phenyl](2,5-disulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-N-ethylethanaminium inner salt sodium salt (Isosulfan blue) of formula I. It also relates to highly pure novel crystalline form of Isosulfan blue hydrate and its process for the preparation thereof. It also relates to an improved process for the preparation of Isosulfan blue sodium hydrate having not more than 0.2% of desethyl impurity of formula A. ##STR00001##

Provided is an improved process for the preparation N-[4-[[4-(diethyl amino) phenyl](2,5-disulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-N-ethylethanaminium inner salt sodium salt (Isosulfan blue) of formula I. It also relates to highly pure novel crystalline form of Isosulfan blue hydrate and its process for the preparation thereof. It also relates to an improved process for the preparation of Isosulfan blue sodium hydrate having not more than 0.2% of desethyl impurity of formula A. ##STR00001##

Salicylic acid-based polymeric CEST contrast agents targeting prostate-specific membrane antigen, pharmaceutical composition comprising the same and methods of use thereof are disclosed.

Salicylic acid-based polymeric CEST contrast agents targeting prostate-specific membrane antigen, pharmaceutical composition comprising the same and methods of use thereof are disclosed.

The present invention relates to a method for preparing a modification polymerization initiator with a high conversion ratio by minimizing side reactions. According to the method for preparing a modification polymerization initiator, a modification polymerization initiator which may easily initiate polymerization and provide a polymer with a functional group having affinity with a filler, may be prepared. Particularly, by performing the method using a continuous reactor, the production of by-products may be decreased, and as a result, the conversion ratio may be increased and a modification polymerization initiator with high purity may be prepared in a high yield.