Crystalline difucosyllactose, useful in a pharmaceutical composition and a nutritional composition, is disclosed.

Crystalline difucosyllactose, useful in a pharmaceutical composition and a nutritional composition, is disclosed.

This specification relates to a process for crystallizing an oligosaccharide, particularly a human milk oligosaccharide, and, more particularly, 2′-fucosyllactose (“2′-FL”). This specification also relates to compositions (e.g., crystalline products) produced using such a process.

This specification relates to a process for crystallizing an oligosaccharide, particularly a human milk oligosaccharide, and, more particularly, 2′-fucosyllactose (“2′-FL”). This specification also relates to compositions (e.g., crystalline products) produced using such a process.

High-purity hydrous D-allose crystals and a method of efficiently obtaining the crystals are provided. To a D-allose-containing solution having a purity of D-allose of at least 80% (g/g) in a solute, in a metastable region in a supersaturated state of 30° C. or less, D-allose seed crystals are added. Then, the temperature of the solution is lowered by 10° C. or more for cooling and crystallization to initially obtain “hydrous D-allose crystals”, and the crystallization water thereof is removed in a specified temperature zone to obtain novel “anhydrous D-allose crystals”.

Disclosed in the present disclosure are methods for enzymatic production of glucosamine salts and the purification methods thereof, and belongs to the technical field of biological engineering. In the present disclosure, liquid containing N-acetylglucosamine is used as a raw material, subjected to hydrolysis with deacetylase to obtain glucosamine and acetic acid, and followed by elution on a cation exchange column with an acidic eluent and separation to obtain the glucosamine salt. Meanwhile, a by-product, namely sodium acetate, is recovered by anion exchange. The obtained glucosamine salt is subjected to concentration, crystallization, decolorization, and drying to obtain a high-purity glucosamine salt crystal. According to the present disclosure, processes for recycling of an enzyme, a residual substrate, and acetic acid are combined. Moreover, the loss rate of resin is low under operation conditions at room temperature, and the production of a hydrochloric acid waste liquid is extremely low.

Disclosed in the present disclosure are methods for enzymatic production of glucosamine salts and the purification methods thereof, and belongs to the technical field of biological engineering. In the present disclosure, liquid containing N-acetylglucosamine is used as a raw material, subjected to hydrolysis with deacetylase to obtain glucosamine and acetic acid, and followed by elution on a cation exchange column with an acidic eluent and separation to obtain the glucosamine salt. Meanwhile, a by-product, namely sodium acetate, is recovered by anion exchange. The obtained glucosamine salt is subjected to concentration, crystallization, decolorization, and drying to obtain a high-purity glucosamine salt crystal. According to the present disclosure, processes for recycling of an enzyme, a residual substrate, and acetic acid are combined. Moreover, the loss rate of resin is low under operation conditions at room temperature, and the production of a hydrochloric acid waste liquid is extremely low.

A method for producing high purity D-psicose crystals having a purity of 98% (w/w) or more and a grain size of MA200 or more. The method includes: removing impurities from a D-psicose solution to obtain a purified D-psicose solution; concentrating the purified D-psicose solution; cooling the concentrated D-psicose solution to 30° C. to 40° C. through a heat exchanger; seed crystallizing the D-psicose solution at 30° C. to 40° C. to obtain a seed crystallized massecuite; and full-scale crystallizing the seed crystallized massecuite. The method can produce pure D-psicose crystals in a suitable form for industrial application through an economical crystallization process from the D-psicose solution without using organic solvents.

A method for producing high purity D-psicose crystals having a purity of 98% (w/w) or more and a grain size of MA200 or more. The method includes: removing impurities from a D-psicose solution to obtain a purified D-psicose solution; concentrating the purified D-psicose solution; cooling the concentrated D-psicose solution to 30° C. to 40° C. through a heat exchanger; seed crystallizing the D-psicose solution at 30° C. to 40° C. to obtain a seed crystallized massecuite; and full-scale crystallizing the seed crystallized massecuite. The method can produce pure D-psicose crystals in a suitable form for industrial application through an economical crystallization process from the D-psicose solution without using organic solvents.

The invention relates to a method for the separation of two hydrophilic neutral oligosaccharides from each other with a chromatography on a bromine functionalized polystyrene cross-linked with divinylbenzene (BPS-DVB) stationary medium.