The problem to be solved is to provide a humanized anti-IL-6 receptor antibody MRA-containing formulation which is suitable for subcutaneous administration, wherein dimerization or deamidation is prevented during long-term storage. The present application is directed to a stable antibody-containing liquid formulation characterized by containing arginine and histidine buffer. A method of inhibiting deamidation or dimerization of such an antibody in a concentrated liquid formulation includes histidine buffer in the liquid formulation.

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.

The present invention relates to recombinant protein production in algal cells. In particular, the present invention provides methods for making recombinant polypeptides in association with accumulated lipid particles or chloroplasts. The methods involve producing the recombinant polypeptide as a fusion polypeptide with an oil body protein and the growth of the algal cells under non-homeostatic conditions to form accumulated lipid particles within the algal cells, wherein the algal lipid particles contain the fusion polypeptide.

The present invention relates to recombinant protein production in algal cells. In particular, the present invention provides methods for making recombinant polypeptides in association with accumulated lipid particles or chloroplasts. The methods involve producing the recombinant polypeptide as a fusion polypeptide with an oil body protein and the growth of the algal cells under non-homeostatic conditions to form accumulated lipid particles within the algal cells, wherein the algal lipid particles contain the fusion polypeptide.

Methods and kits for isolation of cell-derived vesicles and their associated macromolecules like nucleic acids, proteins, lipids metabolites etc. from one or more blood, serum, plasma, saliva, urine, cerebrospinal fluid, breast milk, tear, conditioned culture media etc. to assist detection, prevention, and understanding of disease biology. The invention offers various advantages including simple technical solutions which are cost-effective, time-saving and scalable for large industrial outputs.

Methods and kits for isolation of cell-derived vesicles and their associated macromolecules like nucleic acids, proteins, lipids metabolites etc. from one or more blood, serum, plasma, saliva, urine, cerebrospinal fluid, breast milk, tear, conditioned culture media etc. to assist detection, prevention, and understanding of disease biology. The invention offers various advantages including simple technical solutions which are cost-effective, time-saving and scalable for large industrial outputs.

The present invention provides methods for extraction of amino acid-rich fractions from keratin-containing biological materials, and to amino acid-rich protein fractions generated by the methods described herein. The methods involve forming a reaction mixture with keratin containing material at a pH of 1.1 to 6.9. The reaction mixture is exposed to an energy source, such as microwaves, sufficient to degrade the keratin. The amino acid mixture which is subsequently extracted is substantially insoluble.

A microfluidic control system and method for separating flexible particles such as cell vesicles or biomacromolecules such as exosomes in a sample. The system of the present invention comprises one or more ultrahigh frequency acoustic resonators. The ultrahigh frequency acoustic resonators are capable of generating in a fluid channel an acoustic wave of which the frequency is about 0.5-50 GHz and propagated towards a wall opposite the fluid channel. By adjusting the power of the generated acoustic wave and/or the speed at which a conditioning solution flows through an acoustic wave area, flexible particles in a specified range are pushed to and remain at the top part of the flow channel in the acoustic wave area, while flexible particles outside of the specified range go downstream via the acoustic wave area to be collected, thus capturing or releasing the flexible particles in a solution such as cell vesicles or biomacromolecules, particularly exosomes.

Isolated antigen binding molecules that specifically binds to a molecule comprising an amino acid sequence selected from the group consisting of GSTSGSGKPGSGEGSTKG (SEQ ID NO: 1), GSGKPGSGEG (SEQ ID NO: 2), GKPGSGEG (SEQ ID NO: 3), SGKPGSGE (SEQ ID NO: 499) and KPGSG (SEQ ID NO: 500) are provided. The antigen binding molecules can be used in the methods provided herein.

Isolated antigen binding molecules that specifically binds to a molecule comprising an amino acid sequence selected from the group consisting of GSTSGSGKPGSGEGSTKG (SEQ ID NO: 1), GSGKPGSGEG (SEQ ID NO: 2), GKPGSGEG (SEQ ID NO: 3), SGKPGSGE (SEQ ID NO: 499) and KPGSG (SEQ ID NO: 500) are provided. The antigen binding molecules can be used in the methods provided herein.