The application generally relates to bioproduction of molecules of interest in microorganisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to express or overexpress a mutant of a bubblegum-type acyl-CoA synthase, and uses thereof.

The present invention relates to the field of stem cells. More specifically, the present invention provides compositions and methods for using optogenetics to sustain the pluripotency of stem cells. In one embodiment, a vector comprises a nucleotide sequencing encoding a fusion protein comprising the intracellular domain of fibroblast growth factor 1 receptor (FGFR1) and a photoactivatable domain.

The present invention relates to the field of stem cells. More specifically, the present invention provides compositions and methods for using optogenetics to sustain the pluripotency of stem cells. In one embodiment, a vector comprises a nucleotide sequencing encoding a fusion protein comprising the intracellular domain of fibroblast growth factor 1 receptor (FGFR1) and a photoactivatable domain.

A protein for visual color recognition and the like, the protein having a channel activity and including an amino acid residue different from the amino acid residue present in a first amino acid sequence represented by SEQ ID NO: 1, at a position or positions corresponding to one or two or more selections from the group consisting of the following positions in the first amino acid sequence: positions 53, 83, 87, 117, 120, 124, 137, 139, 142, 143, 146, 150, 169, 173, 177, 198, 204, 216, 217, 218, 231, 238, 245, and 247.

A mutant algal strain showing upregulation of mRNA transcripts encoding urea carboxylase, Δ-15-ω3-desaturase and downregulation of mRNA transcripts of gene encoding triacylglycerol lipase is provided herein. The mutant algal strain of the present disclosure is tolerant to low temperature and thus can be grown over a wide temperature range. The strain shows enhanced biomass and fatty acid production and enhanced growth rate and nitrogen metabolism over a wide temperature range of about 10° C. to about 37° C., wherein the enhancement is in comparison to the wild type algal strain. A method of obtaining the mutant algal strain and a method of producing industrially relevant products such as fatty acids from the mutant algal strain also are provided herein.

A mutant algal strain showing upregulation of mRNA transcripts encoding urea carboxylase, Δ-15-ω3-desaturase and downregulation of mRNA transcripts of gene encoding triacylglycerol lipase is provided herein. The mutant algal strain of the present disclosure is tolerant to low temperature and thus can be grown over a wide temperature range. The strain shows enhanced biomass and fatty acid production and enhanced growth rate and nitrogen metabolism over a wide temperature range of about 10° C. to about 37° C., wherein the enhancement is in comparison to the wild type algal strain. A method of obtaining the mutant algal strain and a method of producing industrially relevant products such as fatty acids from the mutant algal strain also are provided herein.

Provided herein are compositions comprising light-activated chimeric proteins expressed on plasma membranes and methods of using the same to selectively depolarize excitatory or inhibitory neurons.

Provided herein are compositions comprising light-activated chimeric proteins expressed on plasma membranes and methods of using the same to selectively depolarize excitatory or inhibitory neurons.

A fusion chimeric protein is described herein that can assemble a functional carboxysome core, which is able to fix carbon by taking atmospheric carbon dioxide and converting it into useful carbon-containing compounds such as 3-phosphoglycerate (3-PGA).

A fusion chimeric protein is described herein that can assemble a functional carboxysome core, which is able to fix carbon by taking atmospheric carbon dioxide and converting it into useful carbon-containing compounds such as 3-phosphoglycerate (3-PGA).