A method of making heterogeneous biocatalysts can include admixing a protein dissolved in an aqueous buffer with an imprinting compound dissolved in a water-immiscible imprinting solvent, wherein the imprinting compound is hydrophobic and enters and remains in the active site of the protein due to hydrophobic interaction. A water-miscible organic solvent can then be added to precipitate the protein having the imprinting compound in the active site as an amorphous solid. The precipitate can be separated and lyophilized and then washed to remove the imprinting compound, thereby providing a heterogeneous biocatalyst.

A method of making heterogeneous biocatalysts can include admixing a protein dissolved in an aqueous buffer with an imprinting compound dissolved in a water-immiscible imprinting solvent, wherein the imprinting compound is hydrophobic and enters and remains in the active site of the protein due to hydrophobic interaction. A water-miscible organic solvent can then be added to precipitate the protein having the imprinting compound in the active site as an amorphous solid. The precipitate can be separated and lyophilized and then washed to remove the imprinting compound, thereby providing a heterogeneous biocatalyst.

Disclosed herein are potency assays for a gene therapy treatment for -thalassemia. Also disclosed herein are methods for measuring relative potency of a drug product.

Disclosed herein are potency assays for a gene therapy treatment for -thalassemia. Also disclosed herein are methods for measuring relative potency of a drug product.