Wheat grain (Triticum aestivum) comprising an embryo and starch, wherein the embryo comprises two identical alleles of an SBEIIa-A gene, two identical alleles of an SBEIIa-B gene and two identical alleles of an SBEIIa-D gene, wherein each of the SBEIIa genes gives rise to an amount of protein (w/w) or a protein having SBEIIa activity which is lower than the corresponding wild-type gene, and at least one of said genes comprises a point mutation, wherein the starch comprises amylose such that the grain has an amylose content of at least 50% (w/w) as a proportion of the extractable starch of the grain.

A method of processing an initial starch containing gluten protein to produce a purified starch having less than 20 parts per million of a gluten protein (i.e., “gluten free”). A slurry of the unpurified starch is treated with an agent to degrade the gluten protein, and then the degraded gluten protein is removed, resulting in a slurry of the purified starch. The slurry of the purified starch is dried, resulting in the purified starch, and the purified starch is tested to confirm that the purified starch meets the standard for being gluten free. The starch is from a member of the tribe Triticeae (e.g., wheat, rye, barley, or triticale) or other plant starch that either naturally contains gluten protein or may be contaminated with gluten protein. The agent is selected from among acids, bases, alcohols, surfactants, proteases, chaotropic agents, reducing agents, and combinations thereof.

A method of processing an initial starch containing gluten protein to produce a purified starch having less than 20 parts per million of a gluten protein (i.e., “gluten free”). A slurry of the unpurified starch is treated with an agent to degrade the gluten protein, and then the degraded gluten protein is removed, resulting in a slurry of the purified starch. The slurry of the purified starch is dried, resulting in the purified starch, and the purified starch is tested to confirm that the purified starch meets the standard for being gluten free. The starch is from a member of the tribe Triticeae (e.g., wheat, rye, barley, or triticale) or other plant starch that either naturally contains gluten protein or may be contaminated with gluten protein. The agent is selected from among acids, bases, alcohols, surfactants, proteases, chaotropic agents, reducing agents, and combinations thereof.

A pollen polysaccharide extract prepared by the following method: mixing rape pollen with water, then heating and stirring for extraction, filtering, mixing filtrates, adding chitosan to a resulting filtrate to obtain a liquid to be clarified, keeping the liquid to be clarified at 60-80° C. for at least 1 h, cooling and allowing to stand, and performing solid-liquid separation to obtain a liquid, namely the pollen polysaccharide extract.

A pollen polysaccharide extract prepared by the following method: mixing rape pollen with water, then heating and stirring for extraction, filtering, mixing filtrates, adding chitosan to a resulting filtrate to obtain a liquid to be clarified, keeping the liquid to be clarified at 60-80° C. for at least 1 h, cooling and allowing to stand, and performing solid-liquid separation to obtain a liquid, namely the pollen polysaccharide extract.

Provided is a microorganism, which is Leuconostoc citreum WSJ-57 deposited under an accession number of CGMCC No. 19201 in the China General Microbiological Culture Collection Center on Dec. 20, 2019.

Provided is a microorganism, which is Leuconostoc citreum WSJ-57 deposited under an accession number of CGMCC No. 19201 in the China General Microbiological Culture Collection Center on Dec. 20, 2019.

The invention relates to a method for producing a heat-modified starch, comprising the steps consisting in: (i) preparing a starch milk having a solids content of between 30 and 40%, preferably between 35 and 37% by weight, (ii) adding a solution of an alkaline agent at a weight concentration of between 25 and 35%, preferably of 30%, so as to obtain a conductivity on the milk of between 4 and 7 mS/cm, (iii) ensuring a contact time of between 0.5 and 2 hours, (iv) filtering and drying the starch milk such that the conductivity of the dried starch resuspended at 20% by weight of solids is between 0.7 and 2.5 mS/cm, (v) heating said dried starch so as to bring it to a temperature of more than 180° C. for a residence time of between 10 and 40 minutes, even more preferentially between 15 and 35 minutes.

Provided are food and drink ingredients produced from wheat grain which has a low level (2-30%) of total starch branching enzyme II activity and an amylose content in the starch of at least 50% (w/w), and processes for producing and using the ingredients. Also provided are foods produced from the ingredients which may be used in humans to improve one or more parameters of metabolic health, bowel health or cardiovascular health.

The instant application provides methods to improve the total starch yield and/or gluten yield from corn kernels in a wet milling process, the method comprising admixing corn kernels or a fraction of the corn kernels with an enzyme composition comprising an effective amount of one or more hydrolytic enzymes, wherein at least one of said hydrolytic enzymes is selected from the group consisting of a GH30 polypeptide, a GH5 polypeptide or a combination thereof.