The present invention includes methods for preparing anticoagulant polysaccharides using several non-naturally occurring, engineered sulfotransferase enzymes that are designed to react with aryl sulfate compounds instead of the natural substrate, PAPS, to facilitate sulfo group transfer to polysaccharide sulfo group acceptors. Suitable aryl sulfate compounds include, but are not limited to, p-nitrophenyl sulfate or 4-nitrocatechol sulfate. Anticoagulant polysaccharides produced by methods of the present invention comprise N-, 3-O-, 6-O-sulfated glucosamine residues and 2-O sulfated hexuronic acid residues, have comparable anticoagulant activity compared to commercially-available anticoagulant polysaccharides, and can be utilized to form truncated anticoagulant polysaccharides having a reduced molecular weight.

A method for separating and recovering compounds from biomass is provided. In the method, an aqueous extraction fluid in essentially gaseous phase, such as steam, is propagated through a reaction area containing biomass feedstock. When extraction fluid advances through the biomass feedstock, in predetermined reaction conditions, a target compound separates from the essentially solid feedstock matter and travels, with the extraction fluid, towards the end of the reaction area, in where the target compound is recovered in the form of an essentially liquid medium. The method is beneficial for extracting long-chain hemicelluloses from cellulose-containing feedstocks.

A process is disclosed for obtaining citrus fiber from citrus pulp. Citrus fiber is obtained having a c* close packing concentration value of less than 3.8. The citrus fiber can be obtained having a viscosity of at least 1000 mPa.s, wherein said citrus fiber is dispersed in standardized water at a mixing speed of from 800 rpm to 1000 rpm, to a 3 w/w% citrus fiber/standardized water solution, and wherein said viscosity is measured at a shear rate of 5 s-1 at 20° C. Citrus fiber can be obtained having a CIELAB L* value of at least 90. The citrus fiber can be used in food products, feed products, beverages, personal care products, pharmaceutical products or detergent products.

Methods of preparing a hemicellulose-product and a holocellulose-product from a carbohydrate-rich material, including spend coffee grounds, are described. Hemicellulose-products and holocellulose-products produced according to these methods are also described.

A vegetable product obtained from vegetable matter selected from at least one of root, tubers and leaves of a plant having inulin as a reserve carbohydrate, wherein the product includes particles having particle sizes in the range of 0.5 to 10 mm and including inulin and cell walls having pectin, hemi-cellulose and cellulose, wherein the inulin is contained within the cell walls, and the product is obtained by a process including the steps of: a) wounding the vegetable matter, b) treating the vegetable matter with an aqueous solution having an antioxidant and/or a textural support agent and heating the vegetable matter to a temperature of 40 to 90° C. and c) mechanically reducing the size of the vegetable matter and drying the vegetable matter to obtain the product.

High-molecular-weight fucan compositions comprising a therapeutically effective, medically acceptable fucan in a composition comprising wherein the fucan, for example, has a molecular weight distribution in which more than 60% w/w of the composition has a molecular weight above 100 kDa.

Present invention relates to a process to reduce butane sultone hydrolysis products (4-hydroxybutane-1-sulfonic acid, and bis(4-sulfobutyl) ether disodium) in sulfobutylether cyclodextrin reaction mixtures achieved with the combined use of a strong anion exchange resin having dialkyl 2-hydroxyethyl ammonium hydroxide functionality and a cation exchange resin.

Compositions, methods, systems, etc., are provided for modified and/or purified fucans and corresponding fucan-containing compositions that inhibit fibrous adhesions among other advantages. The purified/modified fucans and fucan compositions have a reduced level of non-fucan components or impurities such as those found in a starting fucan composition. Such reduced undesirable components or impurities include, for example, undesired components bound to the fucan and compounds in the composition that are not a part of or bound to the fucan.

An object of the present invention is to provide a method for easily extracting and efficiently recovering xylan from wood.

A xylan-containing material is produced by a method comprising the steps of: (a) adding an acid and/or carbon dioxide to a black liquor discharged during a soda cooking step of wood chips including hardwood chips to adjust the pH of the black liquor to be in the range of 1 to 9, thereby giving a suspension; (b) dehydrating and washing an insoluble matter generated in the suspension to separately collect the insoluble matter; and (c) adding an organic solvent to the insoluble matter obtained at the step (b) to give a suspension, and separately collecting a xylan-containing material made of the insoluble matter present in the suspension by solid/liquid separation.

The invention comprises a method of industrial extraction of alginates from brown seaweeds of the family Sargassaceae of the order Fucales, as well as the product resulting from the method. The method includes the following stages: preparation of raw materials, crushing of the algae, pressure boiling of the algae, mixing the boiled algae with water, purification of the diluted algal mass, extraction of alginic acid, washing of the alginic acid, dehydration of the alginic acid, treatment of the alginic acid with sodium hydrogen carbonate, clarification of resultant sodium alginate solution, precipitation of sodium alginate gel, and drying of the sodium alginate gel.