A method for producing membrane polysaccharides from an organism selected from micro-organisms, unicellular organisms and filamentous fungi, the method including at least one step of extracting the membrane polysaccharides as well as a smaller-scale extraction of the soluble proteins, by mechanical treatment of the organism in a ball mill or by physical treatment of the organism by means of ultrasounds.

The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method using magnetic beads containing negatively charged carboxyl groups extending from the surface of the magnetic beads.

Oligosaccharides from bovine milk, whey and dairy products, and methods of producing bovine milk oligosaccharides are provided.

The present disclosure relates, according to some embodiments, to methods for recovering multiple products from industrial-scale production of a biomass of an aquatic species. Specifically, the present disclosure relates, in some embodiments, to methods for cultivating Lemna for extracting a protein concentrate, a biocrude, and/or a carbohydrate-rich meal. In some embodiments, a process of recovering at least one product from biomass of an aquatic species may comprise: lysing a biomass to generate a lysed biomass; separating the lysed biomass to generate a juice and a first solid phase; filtering the juice to generate a filtered juice and a second solid phase; clarifying at least one juice to generate a clarified juice and a third solid phase, wherein the clarified juice comprises a soluble protein; coagulating the soluble protein from the clarified juice to generate a liquor comprising a wet protein concentrate; and separating the wet protein concentrate from the liquor.

A Grifola frondosa polysaccharide F2 with hypoglycemic activity, process for preparation and use thereof. The process for preparation of Grifola frondosa polysaccharide F2 is as follows: The fruit bodies of Grifola frondosa were homogenized to a fine powder and extracted with hot water. The mixture was filtered and precipitated with absolute ethanol. The precipitation was obtained. The said precipitation was applied on DEAE Sepharose Fast chromatographic column, equilibrated with Tris-HCl (10 mM, pH=8.0), collecting the efficient eluting peak to obtain the fraction F1; eluted with Tris-HCl (10 mM, pH=8.0) which contains 0.1M NaCl, fraction F2 was obtained; then concentrated under reduced pressure, dialyzed and lyophilized, Grifola frondosa polysaccharide F2 was obtained. This isolates a new Grifola frondosa polysaccharide F2 with hypoglycemic activity from the fruit bodies of Grifola frondosa. The Grifola frondosa polysaccharide F2 can be used in manufacturing a drug for treating diabetes. The polysaccharide makes a foundation for developing new anti-diabetes agents.

The present invention provides a method to prepare polysaccharides from Ganoderma lucidum. The prepared polysaccharides can reduce hyperglycemia and improve insulin sensitivity in humans and animals, and can therefore be used to prevent and treat type 2 diabetes.

The present invention provides a method to prepare polysaccharides from Ganoderma lucidum. The prepared polysaccharides reduce body weight and fat accumulation in laboratory animals, and can therefore be used to prevent and treat obesity.

The present disclosure provides a low-molecular-weight Tremella aurantialba glucuronoxylomannan (LTAG) as well as a preparation method and an application thereof, and specifically relates to the technical field of medicine. The LTAG provided in the present disclosure has a weight-average molecular weight of 8,000-24,000 Da. In the method of preparing LTAG as provided in the present disclosure, Tremella aurantialba glucuronoxylomannan is depolymerized by peroxides so as to get low-molecular-weight products, which are then exchanged into pharmaceutically acceptable salts through cation exchange resins. The resulting LTAG has a clear structure, a low viscosity and a good solubility, has a strong immune-enhancing activity, and is capable of acting on TLR4 receptor-activated macrophagocytes and promoting the production of various immune factors, so it can be used in the prevention and/or treatment of immunodeficiency-related diseases.

The present invention provides capsular polysaccharides from Streptococcus pneumoniae serotypes identified using NMR. The present invention further provides polysaccharide-protein conjugates in which capsular polysaccharides from one or more of these serotypes are conjugated to a carrier protein such as CRM197. Polysaccharide-protein conjugates from one or more of these N serotypes may be included in multivalent pneumococcal conjugate vaccines having polysaccharides from multiple additional Steptococcus pneumoniae serotypes.

The present invention discloses a method for separating and purifying polysaccharides from Ganoderma spores. The method includes ultrasonically extracting defatted Ganoderma spore powder and filtering with a plate and frame filter press to obtain a filtrate, and then precipitating the filtrate by passing sequentially through ethanol with a mass concentration of 75% and ethanol with a mass concentration of 85%, and filtering, to obtain polysaccharides which have a significant inhibitory effect on transplanted tumors in animals.