An object of the present invention is to provide a cryopreservation vessel which has a coating that is capable of inhibiting the adhesion of cells or proteins and is resistant not only to aqueous solvents but also to organic solvents (for example, cryoprotectants, in particular, dimethyl sulfoxide). The invention provides a cell or protein cryopreservation vessel including, on at least a portion of the surface thereof, a coating that includes a repeating unit containing a group represented by the following formula (a), and a repeating unit containing a group represented by the following formula (b) (wherein U.sup.a1, U.sup.a2, U.sup.a3, U.sup.b1, U.sup.b2, U.sup.b3 and An.sup.− are as defined in the description and the claims).

An active energy ray-curable resin composition containing: a reaction product (X) of a component (A) and a component (B) below: (A) at least one selected from the group consisting of meta-xylylenediamine and para-xylylenediamine; (B) at least one selected from the group consisting of unsaturated carboxylic acids represented by the following general formula (1) and derivatives of the unsaturated carboxylic acids: ##STR00001## wherein, in the formula (1), R.sup.1 and R.sup.2 each independently represent a hydrogen atom, an alkyl group having from 1 to 8 carbon atoms, an aryl group having from 6 to 12 carbon atoms, or an aralkyl group having from 7 to 13 carbon atoms; (C) a compound having at least one group selected from the group consisting of a glycidyl group and an isocyanate group, and an ethylenically unsaturated bond-containing group; and (D) a phosphoric acid derivative having an ethylenically unsaturated bond-containing group.

An active energy ray-curable resin composition containing: a reaction product (X) of a component (A) and a component (B) below: (A) at least one selected from the group consisting of meta-xylylenediamine and para-xylylenediamine; (B) at least one selected from the group consisting of unsaturated carboxylic acids represented by the following general formula (1) and derivatives of the unsaturated carboxylic acids: ##STR00001## wherein, in the formula (1), R.sup.1 and R.sup.2 each independently represent a hydrogen atom, an alkyl group having from 1 to 8 carbon atoms, an aryl group having from 6 to 12 carbon atoms, or an aralkyl group having from 7 to 13 carbon atoms; (C) a compound having at least one group selected from the group consisting of a glycidyl group and an isocyanate group, and an ethylenically unsaturated bond-containing group; and (D) a phosphoric acid derivative having an ethylenically unsaturated bond-containing group.

The multilayer coating film formation method for forming a multilayer coating film on a substrate comprises the steps of: applying an undercoat paint composition (X) comprising an antioxidant (a1) to a substrate to form at least one layer of an uncured first coating film; applying a topcoat paint composition (Y) containing a carboxy-containing polymer (b1) and an epoxy-containing acrylic resin (b2) to the first coating film to form an uncured second coating film; and simultaneously heating the uncured first coating film and the uncured second coating film to cure the films.

The multilayer coating film formation method for forming a multilayer coating film on a substrate comprises the steps of: applying an undercoat paint composition (X) comprising an antioxidant (a1) to a substrate to form at least one layer of an uncured first coating film; applying a topcoat paint composition (Y) containing a carboxy-containing polymer (b1) and an epoxy-containing acrylic resin (b2) to the first coating film to form an uncured second coating film; and simultaneously heating the uncured first coating film and the uncured second coating film to cure the films.

Zwitterionic copolymers, coating compositions (e.g., aqueous coating compositions and articles containing such copolymers, and methods of coating such coating compositions; wherein the copolymer includes: (a) first monomeric units derived from monomers of Formula (I) CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—Y (I) or salts thereof, wherein: R.sup.1 is hydrogen or methyl; X is oxy or —NH—; R.sup.2 is an alkylene optionally including catenary oxygen; R.sup.3 is an alkylene; Q is —(CO)O—, —NR.sup.4—(CO)—NR.sup.4—, or —(CO)—NR.sup.4—; R.sup.4 is hydrogen or alkyl; n is equal to 0 or 1; and Y is phosphonic acid, phosphonate, phosphoric acid, or phosphate; and (b) second monomeric units derived from monomers of Formula (II) CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—[NR.sup.5R.sup.6]+—R.sup.7—Z— (II) wherein: R.sup.1 is hydrogen or methyl; X is oxy or —NH—; R.sup.2 is alkylene optionally including catenary oxygen; R.sup.3 is alkylene; Q is —(CO)O—, —NR.sup.4—(CO)—NR.sup.4—, or —(CO)—NR4-; R.sup.4 is hydrogen or alkyl; n is equal to 0 or 1; R.sup.5 and R.sup.6 are each independently an alkyl, aryl, or a combination thereof, or R.sup.5 and R.sup.6 both combine with the nitrogen to which they are both attached to form a heterocyclic ring having 3 to 7 ring members; R.sup.7 is alkylene; and Z″ is carboxylate or sulfonate.
CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3-].sub.n—Y  (I)
CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—[NR.sup.5R.sup.6].sup.+—R.sup.7—Z.sup.−  (II)

Zwitterionic copolymers, coating compositions (e.g., aqueous coating compositions and articles containing such copolymers, and methods of coating such coating compositions; wherein the copolymer includes: (a) first monomeric units derived from monomers of Formula (I) CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—Y (I) or salts thereof, wherein: R.sup.1 is hydrogen or methyl; X is oxy or —NH—; R.sup.2 is an alkylene optionally including catenary oxygen; R.sup.3 is an alkylene; Q is —(CO)O—, —NR.sup.4—(CO)—NR.sup.4—, or —(CO)—NR.sup.4—; R.sup.4 is hydrogen or alkyl; n is equal to 0 or 1; and Y is phosphonic acid, phosphonate, phosphoric acid, or phosphate; and (b) second monomeric units derived from monomers of Formula (II) CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—[NR.sup.5R.sup.6]+—R.sup.7—Z— (II) wherein: R.sup.1 is hydrogen or methyl; X is oxy or —NH—; R.sup.2 is alkylene optionally including catenary oxygen; R.sup.3 is alkylene; Q is —(CO)O—, —NR.sup.4—(CO)—NR.sup.4—, or —(CO)—NR4-; R.sup.4 is hydrogen or alkyl; n is equal to 0 or 1; R.sup.5 and R.sup.6 are each independently an alkyl, aryl, or a combination thereof, or R.sup.5 and R.sup.6 both combine with the nitrogen to which they are both attached to form a heterocyclic ring having 3 to 7 ring members; R.sup.7 is alkylene; and Z″ is carboxylate or sulfonate.
CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3-].sub.n—Y  (I)
CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—[NR.sup.5R.sup.6].sup.+—R.sup.7—Z.sup.−  (II)

Zwitterionic copolymers, coating compositions (e.g., aqueous coating compositions and articles containing such copolymers, and methods of coating such coating compositions; wherein the copolymer includes: (a) first monomeric units derived from monomers of Formula (I) CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—Y (I) or salts thereof, wherein: R.sup.1 is hydrogen or methyl; X is oxy or —NH—; R.sup.2 is an alkylene optionally including catenary oxygen; R.sup.3 is an alkylene; Q is —(CO)O—, —NR.sup.4—(CO)—NR.sup.4—, or —(CO)—NR.sup.4—; R.sup.4 is hydrogen or alkyl; n is equal to 0 or 1; and Y is phosphonic acid, phosphonate, phosphoric acid, or phosphate; and (b) second monomeric units derived from monomers of Formula (II) CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—[NR.sup.5R.sup.6]+—R.sup.7—Z— (II) wherein: R.sup.1 is hydrogen or methyl; X is oxy or —NH—; R.sup.2 is alkylene optionally including catenary oxygen; R.sup.3 is alkylene; Q is —(CO)O—, —NR.sup.4—(CO)—NR.sup.4—, or —(CO)—NR4-; R.sup.4 is hydrogen or alkyl; n is equal to 0 or 1; R.sup.5 and R.sup.6 are each independently an alkyl, aryl, or a combination thereof, or R.sup.5 and R.sup.6 both combine with the nitrogen to which they are both attached to form a heterocyclic ring having 3 to 7 ring members; R.sup.7 is alkylene; and Z″ is carboxylate or sulfonate.
CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3-].sub.n—Y  (I)
CH.sub.2═CR.sup.1—(CO)—X—R.sup.2—[-Q-R.sup.3—].sub.n—[NR.sup.5R.sup.6].sup.+—R.sup.7—Z.sup.−  (II)

Two part curable compositions are provided which include a first part comprising a (meth)acrylate component and an amine and a second part comprising a fatty acid peroxide. Further disclosed are methods of preparing a two part adhesive composition using the two part curable compositions and methods of bonding surfaces using the two part curable compositions.

Two part curable compositions are provided which include a first part comprising a (meth)acrylate component and an amine and a second part comprising a fatty acid peroxide. Further disclosed are methods of preparing a two part adhesive composition using the two part curable compositions and methods of bonding surfaces using the two part curable compositions.