The disclosure provides for a container system and method for the cryopreservation and resuspension of a body fluid. The container system may include a cryopreservation container comprising the body fluid in a cryopreservation liquid, a resuspension container comprising a resuspension solution, a connection tube sterilely connecting the cryopreservation container and the resuspension container, and at least one shut-off element actively associated with the connection tube.

A shock absorbing container to protect cryogenically frozen biological material includes an outer case, which includes a first outer panel and a second outer panel, the first outer panel and the second outer panel movable relative to each other between a closed position and an open position, the first outer panel and the second outer panel defining a storage space in the closed position, the first outer panel having a first side facing the storage space, the second outer panel having a first side facing the storage space, wherein moving the first outer panel and the second outer panel into the open position creates or enlarges an opening to access the storage space. A first foam panel is on the first side of the first outer panel, and a second foam panel on the first side of the second outer panel.

Provided a vitrification freezing carrier includes a tube body of a hollow structure with an opened end provided with a matching part, a tube cap provided with a covering part of a hollow structure with one end closed and matching the matching part and provided with an extending part connected to the covering part, a carrying rod arranged on a side, facing an interior of the tube body, of the covering part and capable of extending into the tube body, and an auxiliary sampling mechanism including a carrier body provided with a locking part configured to be matched with the covering part to drive the covering part to move and an elastic reset assembly arranged on the carrier body and including a driving part and an elastic part driven by the driving part and capable of being selectively connected to the extending part in a matched manner.

Provided is a method for freezing a cell aggregate including neural cells. Provided is a method for freezing a cell aggregate including neural cells and having a three-dimensional structure, which comprises following steps (1) and (2): (1) soaking the cell aggregate including neural cells in a cryopreservation solution at 0° C. to 30° C. prior to freezing to prepare a cryopreservation solution-soaked cell aggregate; and (2) freezing the cell aggregate including neural cells in vapor phase of a liquid nitrogen container having a temperature of −150° C. or less.

The invention relates to an aqueous solution for cell preservation of preferably mammalian cells, which can be used for cell preservation as a cryoprotectant or as a pharmaceutical product or excipient. Furthermore, the invention relates to a method for preserving cells using the aqueous solution for cell preservation, and to a method for defrosting cells which are frozen in the aqueous solution.

An organ preservation system having; an organ chamber with a perfusate reservoir, a pump arranged to circulate perfusate, from the reservoir and passes the perfusate through a dialysis filter, oxygenator, and temperature and pressure sensors prior to entering the chamber where an organ is perfused. The organ rests on the platform such that perfusate leaving the organ flows into the perfusate reservoir. The dialysis filter having permeable tubes which allow perfusate constituents to be exchanged with dialysate flowing through the dialysis filter. The dialysate pass through an ion exchange resin removing selected constituents or waste products from the dialysate by absorption by the ion exchange resin. Following waste absorption, the dialysate is recycled to the dialyzer to again remove waste. Removing waste products from the perfusate by dialysis followed by removal of the waste products from the dialysate with the exchange resin, enables dialysate reuse for extended duration.

The present invention is directed to methods for collecting and processing autografts, processed autografts, kits for collecting and transporting autografts, and tools for preparing autografts. It is also directed to autologous bone grafts, and methods of preparing them.

Disclosed herein are methods and compositions for the cryopreservation of stem cells, such as stem-cell derived retinal pigment epithelial cells, that have been seeded onto and cultured on a substrate, such as a polymeric substrate. Such cryopreserved stem cells are useful for cell therapies, such as treatment of ocular damage or disease.

A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.

Vial assemblies comprising a tubular body and a flexible bottom or flexible liner are described herein, wherein the flexible bottom or liner is configured to compress at least partially upon the application of force. Methods for storing and removing frozen samples from such vial assemblies are also described herein.