A composite material film for expanding circulating tumor cells ex vivo and a preparation method thereof, a kit and a method for expanding circulating tumor cells ex vivo, a method for detecting an effect of a drug, and a cryopreservation solution are provided. The preparation method includes: mixing one or more kinds of particles and a solvent to form a mixed liquid, in which the particles are selected from the group consisting of metal particles, metal oxide particles, silicon oxide particles and combinations thereof; placing the mixed liquid on a substrate to form a particle layer; adding a medium material to the particle layer, in which the medium material is selected from the group consisting of styrene and its derivatives, polyester monomers, silicon oxide compounds and combinations thereof; and polymerizing the medium material to form a medium layer to fix the particle layer on the substrate.

A cryogenic fluid composition may include water (H20), and at least one salt. The ratio of water to the at least one salt is approximately between 1% and 6% salt with the remainder water. A cryogenic fluid production device may include a cylindrical housing, and a heat exchanger disposed within the cylindrical housing. The heat exchanger may include an inlet, a channel, and an outlet. A coolant may be conveyed through the inlet, the channel, and the outlet of the heat exchanger. The cryogenic fluid production device may further include an interior wall, and an auger disposed within the interior wall of the heat exchanger.

Among the various aspects of the present disclosure is the provision of a media for cryopreserved cells and methods of making and using same. An aspect of the present disclosure provides for a cell media formulation comprising (e.g., for day 0) one or more components selected from: MCDB 131; Glutamax; P/S; BSA; Glucose; ZnSO4; an enzyme for digesting DNA (e.g., DNASE1); and/or an apoptosis inhibitor (e.g., BI-6C9).

Compositions, devices, and systems comprising a corneal tissue carrier, wherein the corneal tissue carrier comprises a corneal tissue sample and a fluid; wherein the fluid comprises resazurin. Additionally, methods including measuring cell viability.

Modular blood product storage system for temperature-regulated storage of blood products with a temperature regulation unit for temperature regulation of the blood product storage system, a base unit and at least one agitator unit with an upper connection side, a lower connection side, a movable compartment to receive the blood products and a drive for movement of the compartment, wherein the compartment is arranged between the upper connection side and the lower connection side, wherein the upper connection side of the agitator unit is selectively connectable to the temperature regulation unit or a further agitator unit, and wherein the lower connection side of the agitator unit is selectively connectable to the base unit or a further agitator unit.

A method, device, computer program and related immunoassay are disclosed for assessing the efficacy of a statin selected from, for example, selected from RvT1 (7,13,20-trihydroxy-8,10,14,16Z,18-docosapentaenoic acid), RvT2 (7,12,13-trihydroxy-8,10,14,16Z,19Z-docosapentaenoic acid), RvT3 (7,8,13-trihydroxy-9,11,14,16Z,19Z-docosapentaenoic acid) and RvT4 (7,13-dihydroxy-8,10,14,16Z,19Z-docosapentaenoic acid), for use in the treatment of an inflammatory condition in an individual patient, which comprises measuring the levels of at least one 13-series resolvin in biological samples obtained from the patient before and after administration of the statin, wherein an increase in the level of the resolvin after administration of the statin is indicative of efficacy of the statin. Also disclosed is a method of storing a biological sample to preserve lipid mediators in the sample comprising placing the sample in an organic solvent and storing the sample at a temperature of ≤−75° C.

A device to aid in the production and regeneration of tissues and organs, standing alone, or on or inside of the human body, with a method of fabrication of tissues and organs and use of the device.

Disclosed is an ischaemia-free organ perfusion device and perfusion method. The perfusion device perfuses an isolated organ at normal temperature during whole transplantation, and comprises a first container, a second container, a first flow path, a second flow path, a third flow path and a fourth flow path. The perfusion device and the perfusion method are capable of maintaining the blood flow of the isolated organ during whole transplantation without interruption by machine perfusion, recovering the blood of the organ, effectively avoiding the interruption of blood supply for the organ during the whole transplantation, and improving the prognosis of organ transplantation.

The disclosure provides for cryopreservation compositions and methods of use thereof. Aspects of the present disclosure provide for cryopreservation compositions useful in the freezing and thawing of cell therapies. Other aspects of the present disclosure provide for systems for use in the cryopreservation cell therapies. Still other aspects of the present disclosure provide for methods of cryopreserving cell therapies.

The present invention, in which RPE cells are suspended in a medium pharmaceutically acceptable as an ocular irrigating/washing solution and containing a poloxamer, achieves improvement of the post-thawing survival rate of cryopreserved RPE cells, improvement of the photoreceptor cell protection effect by RPE cell transplanted immediately after thawing, and prevention of loss of RPE cells in various steps from thawing to transplantation.