The invention is that of systems of methods for preserving vital assets such as human organs in transit. An exemplary system may include a central software application and processor for storing vital asset identifying information in a database and displaying real-time transit information in relation to the asset to authenticated users. Application programming interfaces are provided to allow authenticated users to monitor location and environmental data transmitted from the proximity of the asset to the central software application for processing and display on graphical user interfaces. An exemplary system enables methods of reallocating freight from a first to a second transportation asset to avoid delays and prolonged exposures to adverse environmental conditions. In certain embodiments, unmanned aerial systems may be deployed to intervene in the transit channel and overcome such delays or exposures. In preferred embodiments, historical data is collected and analyzed to help predict human organ transplant outcomes.

Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.

The present invention provides methods for cryopreservation of insect embryos comprising chorion layers and waxy layer, particularly fly embryos including embryos of Black Soldier Fly (Hermetia illucens, BSF) useful in mass rearing of beneficial fly adults. The present invention further provides cryopreserved embryos having an industrially suitable adult recovery and survival rate.

A remote system for monitoring and controlling one or more devices for use in the cryogenic processing of a sample is provided. A remote server capable of transmitting freezing profile data to one or more freezers, transmitting transportation profile data to one or more transportation devices, and transmitting thawing profile data to one or more thawing devices. The remote server is also capable of receiving detected data from the one or more freezers relating to the freezing of a sample in accordance with the freezing profile data, receiving detected data from the one or more transportation devices relating to the transportation of a sample in accordance with the transportation profile data, and receiving detected data from the one or more thawing machines relating to the thawing of a sample in accordance with the thawing profile data.

An organ container has a pouch-shaped body having contraction and expansion properties and an opening. In a no-load condition, a maximum width of the opening is less than a maximum width of the body. On the other hand, the opening is expandable to a state in which the maximum width of the opening becomes greater than the maximum width of the body. Thus, the organ container covers the surface of an organ along the surface of the organ. Accordingly, when an organ is placed in the body cavity of a recipient, the organ container does not expand around the organ. For this reason, the organ container is less likely to hinder work during operation. Moreover, the organ container covers most part of the organ and thereby suppresses a temperature rise in the organ.

This disclosure relates to a method and system for fast freezing of a biological product (such as blood plasma) contained in an individual bag or a plurality of bags. This disclosure relates to a method and system for freezing plasma in an individual bag or a plurality of bags, favoring bottom-up ice-growth, by implementing a differential air flow on top and bottom surfaces of a horizontally placed bag or bags filled with plasma. This disclosure relates to a method and a differential air flow system for freezing plasma, wherein the heat transfer coefficient on a bottom of a bag is at least 10 times larger than at a top. This disclosure relates to a differential air flow system and method for freezing plasma, wherein the differential air flow on top and bottom surfaces of a plasma bag is imposed by at least one fan or blower.

The present disclosure provides methods of preparing a fixed biological sample for use in an assay, wherein the method includes treatment of the sample with a low temperature active protease, optionally, in combination with an un-fixing reagent. The disclosure also provides assay methods, include partition-based methods, for fixed biological sample that use the low temperature protease treatment in combination with an un-fixing reagent. Kits comprising protease compositions, un-fixing agent compositions, and other assay reagents for use in the methods are also provided.

Microbiota restoration therapy compositions and methods for manufacturing, processing, and/or delivering microbiota restoration therapy compositions are disclosed. An example method for manufacturing a microbiota restoration therapy composition may include collecting a human fecal sample and adding a diluent to the human fecal sample to form a diluted sample. The diluent may include a cryoprotectant. The method may also include mixing the diluted sample with a mixing apparatus and filtering the diluted sample. Filtering may form a filtrate. The method may also include transferring the filtrate to a sample bag and sealing the sample bag.

The invention concerns a cryogenic system (1) for vitrifying a substance comprising biological material to be preserved in a receiving region of a thin tube (8), comprising a tank (10) containing a bath (11) of a cryogenic agent and a support member (80) mounted on said tank in a predetermined first position in which said tube is orientated upright, the receiving region being immersed in a liquid zone (17) of said bath, and an opening of said tube being situated above said bath, in a zone of ambient air above said bath, in order to introduce the substance into the tube, the support member being mounted on said tank in a second predetermined position, in which said receiving region is immersed in the liquid zone and said opening is situated immediately above said liquid zone, in a gaseous zone (18) of said bath above said liquid zone and below said zone of ambient air, and being raised from said second position.

Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.