Provided are methods of controlling disassociation of cells from a carrier, compositions, and methods of collecting cells. The methods of controlling disassociation of cells from a carrier may include contacting a polymeric carrier with one or more digesting agents to disassociate at least a portion of a plurality of cells from the polymeric carrier. The polymeric carrier may be crosslinked with a crosslinker including at least one of a redox sensitive moiety, a UV light sensitive moiety, a pH sensitive moiety, and a temperature sensitive moiety.

Amine-functionalized saccharide polymers, including oligosaccharides and polysaccharides, may be effective for promoting clay stabilization in subterranean formations. Oxidative synthesis of amine-functionalized saccharide polymers using sodium hypochlorite pentahydrate as an oxidation reagent may afford a different distribution and type of sites of oxidative opening as compared to other types of oxidation reagents. Amine-functionalized saccharide polymers may be prepared by exposing a saccharide polymer comprising a trans-vicinal diol to an oxidation reagent comprising sodium hypochlorite pentahydrate, reacting the trans-vicinal diol to form a site of oxidative opening bearing at least one aldehyde, exposing the at least one aldehyde to an amine to form an imine intermediate at the site of oxidative opening, and reducing the imine intermediate into a secondary or tertiary amine at the site of oxidative opening.

A method is described for inhibiting marine fouling on a surface. The surface is contacted with a composition comprising a waterborne polyurethane that is crosslinked with an acrylic acid functionalized polysaccharide, water, and a preservative. The composition is then dried to form a deposited film. The polysaccharide may be xanthan.

According to the invention there is provided inter alia a process for the manufacture of a solid object having a surface comprising a layered coating of cationic and anionic polymer wherein the outer coating layer comprises an anticoagulant entity, comprising the steps of: i) treating a surface of the solid object with a cationic polymer; ii) treating the surface with an anionic polymer; iii) optionally repeating steps i) and ii) one or more times; iv) treating the surface with a cationic polymer; and v) treating the outermost layer of cationic polymer with an anticoagulant entity, thereby to covalently attach the anticoagulant entity to the outermost layer of cationic polymer; wherein, the anionic polymer is characterized by having (a) a total molecular weight of 650 kDa-10,000 kDa; and (b) a solution charge density of >4 μeq/g; and wherein, step ii) is carried out at a salt concentration of 0.25 M-5.0 M.

According to the invention there is provided inter alia a process for the manufacture of a solid object having a surface comprising a layered coating of cationic and anionic polymer wherein the outer coating layer comprises an anticoagulant entity, comprising the steps of: i) treating a surface of the solid object with a cationic polymer; ii) treating the surface with an anionic polymer; iii) optionally repeating steps i) and ii) one or more times; iv) treating the surface with a cationic polymer; and v) treating the outermost layer of cationic polymer with an anticoagulant entity, thereby to covalently attach the anticoagulant entity to the outermost layer of cationic polymer; wherein, the anionic polymer is characterized by having (a) a total molecular weight of 650 kDa-10,000 kDa; and (b) a solution charge density of >4 μeq/g; and wherein, step ii) is carried out at a salt concentration of 0.25 M-5.0 M.

Disclosed herein are nonwoven webs comprising a nonwoven substrate and a binder comprising a polysaccharide. In one embodiment, the polysaccharide can comprise poly alpha-1,3-glucan, a poly alpha-1,3-glucan ether compound as disclosed herein, a poly alpha-1,3-glucan ester compound as disclosed herein, a graft copolymer comprising a backbone comprising dextran and poly alpha-1,3-glucan side chains, a crosslinked graft copolymer comprising a backbone comprising dextran and poly alpha-1,3-glucan chains, or a mixture thereof. Also disclosed are articles comprising the nonwoven webs, and methods of making the nonwoven webs.

Disclosed herein are nonwoven webs comprising a nonwoven substrate and a binder comprising a polysaccharide. In one embodiment, the polysaccharide can comprise poly alpha-1,3-glucan, a poly alpha-1,3-glucan ether compound as disclosed herein, a poly alpha-1,3-glucan ester compound as disclosed herein, a graft copolymer comprising a backbone comprising dextran and poly alpha-1,3-glucan side chains, a crosslinked graft copolymer comprising a backbone comprising dextran and poly alpha-1,3-glucan chains, or a mixture thereof. Also disclosed are articles comprising the nonwoven webs, and methods of making the nonwoven webs.

Molded articles are disclosed herein, the molded article comprising a polysaccharide, wherein the polysaccharide comprises i) poly alpha-1,3-glucan; ii) poly alpha-1,3-1,6-glucan; iii) a graft copolymer that comprises (A) a backbone comprising dextran with a weight-average molecular weight (Mw) of at least about 100000 Daltons, and (B) poly alpha-1,3-glucan side chains comprising at least about 95% alpha-1,3-glucosidic linkages; or iv) a composition comprising a poly alpha-1,3-glucan ester compound as disclosed herein. Optionally, the molded articles can further comprise a plasticizer and/or starch. The molded articles can be useful as a container, a handle, packaging, a tray, a bottle, a cup, a sheet, a disposable food packaging item, an automotive part, a casing for an electronic device, or a toy.

Molded articles are disclosed herein, the molded article comprising a polysaccharide, wherein the polysaccharide comprises i) poly alpha-1,3-glucan; ii) poly alpha-1,3-1,6-glucan; iii) a graft copolymer that comprises (A) a backbone comprising dextran with a weight-average molecular weight (Mw) of at least about 100000 Daltons, and (B) poly alpha-1,3-glucan side chains comprising at least about 95% alpha-1,3-glucosidic linkages; or iv) a composition comprising a poly alpha-1,3-glucan ester compound as disclosed herein. Optionally, the molded articles can further comprise a plasticizer and/or starch. The molded articles can be useful as a container, a handle, packaging, a tray, a bottle, a cup, a sheet, a disposable food packaging item, an automotive part, a casing for an electronic device, or a toy.

A malto-dextrin composition with low DE value and low viscosity and the method for making the same is provided. The malto-dextrin comprises a blue value in the range of 0.02 to 0.28; a dextrose equivalent (DE) in the range of 3 to 10; and a viscosity lower than 26.3185*DE{circumflex over ( )}(−0.7593). The method for preparing the malto-dextrin composition comprises: dispersing raw starch in water to obtain a starch-water slurry; preheating the starch-water slurry with a jet-cooker for a first duration at a first temperature above 100° C. having a temperature variation no more than 0.8° C.; hydrolyzing the slurry by treating the slurry with α-amylases for a second duration at a second temperature; and filtering the hydrolyzed slurry to remove insoluble residual proteins and fibers and obtain an un-fractionated malto-dextrin composition.