Compounds of formula I are disclosed:

##STR00001##

wherein X.sup.1, X.sup.2, X.sup.3, X.sup.4 are independently H, F, Cl, Br, I, CN, NO.sub.2, OR.sup.1, SR.sup.1, NR.sup.1R.sup.2, COR.sup.1, COOR.sup.1, CONR.sup.1R.sup.2, PO.sub.3R.sup.1R.sup.2, SO.sub.2R.sup.1, SO.sub.3R.sup.1 or R.sup.3; R.sup.1 and R.sup.2 are, e.g., H, alkyl or aryl or optionally a ring; R.sup.3 is, e.g., alkyl, alkenyl, alkynyl, aryl or cycloalkyl; Y is OR.sup.1, NR.sup.1R.sup.2, or NR.sup.1R.sup.3; Q is O, S, SO.sub.2, NR, C(R.sup.3).sub.2, Si(R.sup.3).sub.2, Ge(R.sup.3).sub.2, P(O)R.sup.3 or P(O)OR.sup.3; Q and X.sup.1 can optionally form part of a ring; L and M are independently OR.sup.1, SR.sup.1, NR.sup.1R.sup.2 and R.sup.3; L and M can optionally form part of a ring; Z is O, S, NR.sup.1, CR.sup.1R.sup.3 or aryl; and Z and X.sup.4 can optionally form part of a ring.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

In a thermal transfer recording sheet including a base material and a colorant layer, the colorant layer includes a yellow dye layer containing a yellow dye, a magenta dye layer containing a magenta dye, and a cyan dye layer containing a cyan dye, and the cyan dye includes a compound represented by Formula (Ia) or Formula (Ib):

##STR00001##

where R.sub.1 to R.sub.7 are substituents, X.sup. is an anion, and the compound of Formula (Ib) has at least one anionic substituent.

In a thermal transfer recording sheet including a base material and a colorant layer, the colorant layer includes a yellow dye layer containing a yellow dye, a magenta dye layer containing a magenta dye, and a cyan dye layer containing a cyan dye, and the cyan dye includes a compound represented by Formula (Ia) or Formula (Ib):

##STR00001##

where R.sub.1 to R.sub.7 are substituents, X.sup. is an anion, and the compound of Formula (Ib) has at least one anionic substituent.

Azetidine substitute rosamines useful for staining mitochondria of formula (I) are disclosed, wherein at least one of Y and Z is a substituted or unsubstituted azetidine group; X is selected from O, S, SO.sub.2, Se, NR.sub.12, P(O)R.sub.12, CR.sub.13R.sub.14, SiR.sub.13R.sub.14, Te, and GeR.sub.13R.sub.14, and there is at least one Q group on the pendant phenyl, the Q group comprising a group selected from halo, maleimidyl, OSO.sub.2R.sub.13 and epoxide. Also disclosed are methods for staining mitochondria involving incubating a sample in a composition comprising the compound, and analysing mitochondria, involving staining a sample of mitochondria, optionally fixing the cells, illuminating the stained sample using light of an appropriate wavelength to fluoresce the compound, and observing or imaging a magnified image of the sample.

##STR00001##

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.