Process for preparing disodium 5,5-indigosulfonate, the feedstock being indigo, this process including the following steps: i) the indigo is subjected to a sulfonation treatment resulting in a mixture that contains 5,5-indigosulfonic acid, this process including: ii) a reduction treatment is applied to the mixture obtained in step i), and optionally a purification step, so as to obtain a composition including leuco-5,5-indigosulfonic acid, iii) the leuco-5,5-indigosulfonic acid is isolated from the composition resulting from step ii), iv) the leuco-5,5-indigosulfonic acid resulting from step iii) is oxidized to give disodium 5,5-indigosulfonate.

Process for preparing disodium 5,5-indigosulfonate, the feedstock being indigo, this process including the following steps: i) the indigo is subjected to a sulfonation treatment resulting in a mixture that contains 5,5-indigosulfonic acid, this process including: ii) a reduction treatment is applied to the mixture obtained in step i), and optionally a purification step, so as to obtain a composition including leuco-5,5-indigosulfonic acid, iii) the leuco-5,5-indigosulfonic acid is isolated from the composition resulting from step ii), iv) the leuco-5,5-indigosulfonic acid resulting from step iii) is oxidized to give disodium 5,5-indigosulfonate.

The present invention relates to an improved process for preparation of Indigo carmine of Formula (I), in high purity, more than 99.5%.

The present invention relates to an improved process for preparation of Indigo carmine of Formula (I), in high purity, more than 99.5%.

Aqueous leucoindigo solution comprising an aromatic amine, in particular aniline or aniline and N-methylaniline, wherein the concentration of the aromatic amine is below 200 ppm, wherein the leucoindigo salt in the solution is in the form of a mixed sodium and potassium salt, wherein the molar ratio of sodium to potassium is in the range of from above 3:1 to 10:1, preferably wherein the concentration of the salt is in the range of from above 25 to 45% by weight, based on the total weight of the solution.

Aqueous leucoindigo solution comprising an aromatic amine, in particular aniline or aniline and N-methylaniline, wherein the concentration of the aromatic amine is below 200 ppm, wherein the leucoindigo salt in the solution is in the form of a mixed sodium and potassium salt, wherein the molar ratio of sodium to potassium is in the range of from above 3:1 to 10:1, preferably wherein the concentration of the salt is in the range of from above 25 to 45% by weight, based on the total weight of the solution.

Method of making an aqueous aniline-free or aniline-free and N-methylaniline-free leucoindigo solution from an aqueous leucoindigo solution comprising aniline or aniline and N-methylaniline, the concentration of aniline or aniline and N-methylaniline being determined according to ISO 14362-1:2017(E), wherein said leucoindigo is in the form of an alkali metal salt, the method comprising at least steps (A) to (C): (A) providing a liquid stream comprising said aqueous leucoindigo solution comprising said amine(s); (B) providing a purification stream; (C) bringing into contact said liquid stream with said purification stream.

Method of making an aqueous aniline-free or aniline-free and N-methylaniline-free leucoindigo solution from an aqueous leucoindigo solution comprising aniline or aniline and N-methylaniline, the concentration of aniline or aniline and N-methylaniline being determined according to ISO 14362-1:2017(E), wherein said leucoindigo is in the form of an alkali metal salt, the method comprising at least steps (A) to (C): (A) providing a liquid stream comprising said aqueous leucoindigo solution comprising said amine(s); (B) providing a purification stream; (C) bringing into contact said liquid stream with said purification stream.

The present disclosure relates to the biosynthesis of indigoid dye precursors and their conversion to indigoid dyes. Specifically, the present disclosure relates to methods of using polypeptides to produce indigoid dye precursors from indole feed compounds, and the use of the indigoid dye precursors to produce indigoid dyes.

The present disclosure relates to the biosynthesis of indigoid dye precursors and their conversion to indigoid dyes. Specifically, the present disclosure relates to methods of using polypeptides to produce indigoid dye precursors from indole feed compounds, and the use of the indigoid dye precursors to produce indigoid dyes.