The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula ##STR00001##
One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.

Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula ##STR00001##
One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.

Provided are a coloring composition including a compound represented by any one of Formulae (1) to (3) shown in the specification, an ink jet ink including the coloring composition, and a textile printing method.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

To provide a photochromic coating composition comprising (A) a polyrotaxane having a composite molecular structure formed by an axial molecule and a plurality of cyclic molecules clathrating the axial molecule, (B) a photochromic compound and (C) a polyurethane resin and/or precursor thereof.

This composition provides a laminate which exhibits excellent photochromic properties (color optical density and fading speed), moldability and surface hardness.

To provide a photochromic coating composition comprising (A) a polyrotaxane having a composite molecular structure formed by an axial molecule and a plurality of cyclic molecules clathrating the axial molecule, (B) a photochromic compound and (C) a polyurethane resin and/or precursor thereof.

This composition provides a laminate which exhibits excellent photochromic properties (color optical density and fading speed), moldability and surface hardness.

The present disclosure relates to a fluorogenic pH-sensitive dye including an aryl compound having a sulfonyl group (SO.sub.2); and an agarose compound covalently bonded to the sulfonyl group (SO.sub.2) of the aryl compound.

Disclosed herein is a fluorescent compound represented by the following general formula and having fluorescence characteristics that vary in the presence of a subject substance,

##STR00001##

wherein R.sub.1 and R.sub.2 each independently represent a carboxyl group, or a substituent containing a carboxyl group, and R.sub.3 and R.sub.4 each independently represent a hydrogen atom, an alkyl group, an alkoxy group, a halogen atom, a hydroxyl group, a carboxyl group, or a substituent containing a carboxyl group.