This disclosure provides a process based on hydrothermal liquefaction (HTL) treatment for co-processing of high-water-content wastewater sludge and other lignocellulosic biomass for co-production of biogas and bio-crude oil. The mixture of waste activated sludge and lignocellulosic biomass such as birchwood sawdust/cornstalk/MSW was converted under HTL conditions in presence of KOH as the homogeneous catalyst. The operating conditions including reaction temperature, reaction time and solids concentration were optimized based on the response surface methodology for the maximum bio-crude oil production. The highest bio-crude oil yield of around 34 wt % was obtained by co-feeding waste activated sludge with lignocellulosic biomass at an optimum temperature of 310 C., reaction time of 10 min, and solids concentration of 10 wt %. The two by-products from this process (bio-char and water-soluble products) can be used to produce energy as well. Water-soluble products were used to produce biogas through Bio-methane Potential Test (BMP) and were found to produce around 800 mL bio-methane cumulatively in 30 days per 0.816 g of total organic carbon (TOC) or 2.09 g of chemical oxygen demand (COD) of water-soluble products.

A method for algae disruption includes: a thermal treatment of microalgae belonging to Heterokontophyta at a pH of 3.5 or more and 9.5 or less and a temperature of 40 C. or more and 65 C. or less; and a physical treatment of the microalgae using a high pressure dispersion apparatus, the physical treatment following the thermal treatment.

A method for algae disruption includes: a thermal treatment of microalgae belonging to Heterokontophyta at a pH of 3.5 or more and 9.5 or less and a temperature of 40 C. or more and 65 C. or less; and a physical treatment of the microalgae using a high pressure dispersion apparatus, the physical treatment following the thermal treatment.

Provided herein is a method for obtaining flowable oil comprising the steps of providing a population of oil-producing microorganisms; recovering oil from the microorganisms, wherein the oil is at a first temperature; reducing the first temperature over a first period of time to a second temperature; and applying mechanical energy to the oil during the first period of time thereby producing the flowable oil.

Provided herein is a method for obtaining flowable oil comprising the steps of providing a population of oil-producing microorganisms; recovering oil from the microorganisms, wherein the oil is at a first temperature; reducing the first temperature over a first period of time to a second temperature; and applying mechanical energy to the oil during the first period of time thereby producing the flowable oil.

A protein fraction and an oxidation stable fat fraction are recovered from poultry containing fat, bone and protein. The poultry is comminuted, solubilized with a food grade acid or base to form a liquid protein fraction and a solid fat fraction. The protein in liquid fraction is precipitated and the protein product retains the color of raw meat.

A protein fraction and an oxidation stable fat fraction are recovered from poultry containing fat, bone and protein. The poultry is comminuted, solubilized with a food grade acid or base to form a liquid protein fraction and a solid fat fraction. The protein in liquid fraction is precipitated and the protein product retains the color of raw meat.

Methods to prepare vegetable oil compositions having an elevated ethyl ester content are provided.

The invention discloses a method for preparing rapeseed oil by a semi-solid aqueous enzymatic process, belonging to the field of functional foods and health care products. The present invention first prepares a semi-solid rapeseed paste with 3.5-4.5% moisture content, which is hydrolyzed by a mixture of pectinase, cellulase and alkaline protease to extract rapeseed oil. The resulting rapeseed oil contains high levels of active ingredients including totaxin, sterol, phenols and beta-carotene. The rapeseed oil of the invention can be added into animal feeds, which helps to reduce animal blood lipid levels and body weight. It can significantly reduce the contents of total triglyceride, total cholesterol and LDC-C in the blood, and, at the same time, increases the level of HDL-C in the blood. In addition, the rapeseed oil prepared by the present invention can relieve hepatic steatosis in hyperlipemia rats.

In accordance with the invention, it was found, surprisingly, that a polyunsaturated fatty acids (PUFAs)-comprising biomass with other feedstuff components can be extruded at a low energy input of 12-28 Wh/kg to give an extrudate with a very high oil load capacity.