A process for production of biofuels from algae can include cultivating an oil-producing algae by promoting sequential photoautotrophic and heterotrophic growth. The method can further include producing oil by heterotrophic growth of algae wherein the heterotrophic algae growth is achieved by introducing a sugar feed to the oil-producing algae. An algal oil can be extracted from the oil-producing algae, and can be converted to form biodiesel.

Disclosed are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition and then recovering the oil from the lysed cell composition. Further disclosed is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described.

A method is provided for the use of a process additive system to improve the recovery of oil from a process stream generated in a bioproduct production process.

A method is provided for the use of a process additive system to improve the recovery of oil from a process stream generated in a bioproduct production process.

The current invention relates to a method for isolating polyunsaturated fatty acids containing lipids from lipid-containing cells.

A temperature controlled plant maceration and extraction system and method is provided comprising an extraction chamber, an end cap at an one end of the extraction chamber, and an inlet gas valve at a second end. A hollow temperature jacket is formed around the extraction chamber from the first wall and an outer wall defining a space there between that is sealed at the one end and second end. A frame having a first and second pass-through axle is provided, one each fixedly mounted on the jacket between the one end and second end of the extraction chamber. Wherein organic matter is introduced via the one end, a solvent is injected into the chamber at the inlet valve on the second end, the extraction chamber is then rotated about the axels enabling the solvent to repeatedly wash over the organic matter performing maceration of organic matter.

This disclosure provides a process based on hydrothermal liquefaction (HTL) treatment for co-processing of high-water-content wastewater sludge and other lignocellulosic biomass for co-production of biogas and bio-crude oil. The mixture of waste activated sludge and lignocellulosic biomass such as birchwood sawdust/cornstalk/MSW was converted under HTL conditions in presence of KOH as the homogeneous catalyst. The operating conditions including reaction temperature, reaction time and solids concentration were optimized based on the response surface methodology for the maximum bio-crude oil production. The highest bio-crude oil yield of around 34 wt % was obtained by co-feeding waste activated sludge with lignocellulosic biomass at an optimum temperature of 310 C., reaction time of 10 min, and solids concentration of 10 wt %. The two by-products from this process (bio-char and water-soluble products) can be used to produce energy as well. Water-soluble products were used to produce biogas through Bio-methane Potential Test (BMP) and were found to produce around 800 mL bio-methane cumulatively in 30 days per 0.816 g of total organic carbon (TOC) or 2.09 g of chemical oxygen demand (COD) of water-soluble products.

The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

The current invention relates to a method of separating lipids from a lipids containing biomass by demulsification under mild conditions, to an oil and to a delipidated biomass as obtained by such a method.