Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.

Extracted olive oil having extremely less olive smell and excellent skin permeability, and having an extremely higher antioxidative property than that of common olive oil. Also provided is a method for producing extracted olive oil including: crushing olive fruits and olive leaves; and producing the extracted olive oil from an obtained paste-like matter, where the olive fruits and the olive leaves are lactic fermented before or after the crushing step or during the crushing step.

The method of producing a lipid composition comprises an extraction treatment step to extract a lipid comprised in a hydrated raw material by using an extraction solvent comprising a polar solvent and a nonpolar solvent, and a separation treatment step to separate an extract solution obtained in the extraction treatment step into a polar solvent phase comprising a first lipid fraction and a nonpolar solvent phase comprising a second lipid fraction.

The present invention relates to the field of methods for purifying fatty acid ethyl esters. According to the present invention, a method for obtaining a ?3 fatty acid ethyl ester, such as EPA and DHA, each as a high purity product at a high yield is provided. In the method according to the present invention, a raw material fat including EPA and DHA is treated with a lipolytic enzyme and ethyl-esterification is performed as needed; the treated substance is fractionated into a glyceride fraction and a free fatty acid fraction; a fraction comprising more EPA ester and a fraction comprising DHA ester are obtained from the respective fractions; the fraction comprising more EPA ester is purified to prepare a high-purity EPA ester; and the fraction comprising more DHA ester is purified to prepare a high-purity DHA ester.

Provided is a nutritionally enhanced derivative (isolate) from Stabilized Rice Bran (SRB) with improved antioxidant, fat and protein levels enhancing both the nutritional and yield values over existing techniques. Also provided is an improved method that utilizes certain enzyme combinations under various time and temperature conditions for extracting these nutritionally enhanced isolates from SRB.

The present invention relates to an apparatus and method for separating glycoglycerolipids and also glycoglycerolipids and glycosphingolipids from a lipid phase that contains glycoglycerolipids and acyl glycerides or glycoglycerolipids and glycosphingolipids and acyl glycerides, in mild conditions with no hydrolysis and while at the same time effectively depleting the lipid phase of said glycoglycerolipids, glycoglycerolipids and glycosphingolipids and their accompanying substances using an aqueous extraction process.

The invention relates to a method of combined cell digestion and extraction of oil-containing seeds having an oil content of >30% by mass, wherein production of a slurry from the oilseeds and ethanol is followed by a combination cell digestion with a subsequent ethanolic extraction, wherein the smaller portion of the oil of the oilseed and the alcohol-soluble constituents present therein are dissolved in the ethanol in accordance with the dissolution capacity of the ethanol for the oil and the alcohol-soluble constituents, a greater portion of the oil is displaced from the seed cells by the ethanol, forming a free oil phase in the ethanol. The free oil phase is removed and, according to the invention, has semi-raffinate quality, reducing the complexity of refining as an advantage of the method of the invention.

The invention relates to a method of combined cell digestion and extraction of oil-containing seeds having an oil content of >30% by mass, wherein production of a slurry from the oilseeds and ethanol is followed by a combination cell digestion with a subsequent ethanolic extraction, wherein the smaller portion of the oil of the oilseed and the alcohol-soluble constituents present therein are dissolved in the ethanol in accordance with the dissolution capacity of the ethanol for the oil and the alcohol-soluble constituents, a greater portion of the oil is displaced from the seed cells by the ethanol, forming a free oil phase in the ethanol. The free oil phase is removed and, according to the invention, has semi-raffinate quality, reducing the complexity of refining as an advantage of the method of the invention.

The invention relates to methods of preconditioning pretreated cellulosic material in the presence of a combination of phenol oxidizing enzyme and glucoamylase. The invention also relates to processes of producing sugars and fermentation products including a preconditioning step. Finally the invention relates to a composition suitable for preconditioning.

Provided is a nutritionally enhanced derivative (isolate) from Stabilized Rice Bran (SRB) with improved antioxidant, fat and protein levels enhancing both the nutritional and yield values over existing techniques. Also provided is an improved method that utilizes certain enzyme combinations under various time and temperature conditions for extracting these nutritionally enhanced isolates from SRB.