Methods and systems for the production and isolation of fatty acid methyl esters (FAMEs) from a lipid source are described. The method includes extracting a lipid from a lipid source and transesterifying the lipid into a FAME. The method may also include fractionating the FAME from the system. A method of selectively transesterifying a lipid into a FAME is also described.

A composition of matter comprises a shelf stable, partially defatted supercritical CO.sup.2 fluid solvent extracted whole grain flour derived from a cracked biomass of perilla frutescens, the flour comprising minerals and 2 to 8 percent of native seed oil, 35 to 45 percent protein, and 35 to 45 percent fiber.

A composition of matter comprises a shelf stable, partially defatted supercritical CO.sup.2 fluid solvent extracted whole grain flour derived from a cracked biomass of perilla frutescens, the flour comprising minerals and 2 to 8 percent of native seed oil, 35 to 45 percent protein, and 35 to 45 percent fiber.

The invention concerns a strain of Schizochytrium mangrovei, filed on 22 Nov. 2012 with the CNCM as number I-4702, having the ability to produce a high quantity of docosahexaenoic acid (or DHA) and palmitic acid, the methods for producing the corresponding biomass containing said lipid compounds of interest, and the biomass containing the products and compositions prepared from this strain.

The invention concerns a strain of Schizochytrium mangrovei, filed on 22 Nov. 2012 with the CNCM as number I-4702, having the ability to produce a high quantity of docosahexaenoic acid (or DHA) and palmitic acid, the methods for producing the corresponding biomass containing said lipid compounds of interest, and the biomass containing the products and compositions prepared from this strain.

The present invention contemplates the creation of a low fluoride crustacean oil processed from a phospholipid-protein complex (PPC) formed immediately upon a crustacean (i.e., for example, krill) catch. Further, the crustacean oil may also have reduced trimethyl amine and/or trimethyl amino oxide content. The process comprises disintegrating the crustaceans into smaller particles, adding water, heating the result, adding enzyme(s) to hydrolyze the disintegrated material, deactivating the enzyme(s), removing solids from the enzymatically processed material to reduce fluoride content of the material, separating and drying the PPC material. Then, using extraction with supercritical CO.sub.2 or supercritical dimethyl ether, and/or ethanol as solvents, krill oil, inter alia, is separated from the PPC. In the extraction the krill oil can be separated almost wholly from the feed material.

The present invention contemplates the creation of a low fluoride crustacean oil processed from a phospholipid-protein complex (PPC) formed immediately upon a crustacean (i.e., for example, krill) catch. Further, the crustacean oil may also have reduced trimethyl amine and/or trimethyl amino oxide content. The process comprises disintegrating the crustaceans into smaller particles, adding water, heating the result, adding enzyme(s) to hydrolyze the disintegrated material, deactivating the enzyme(s), removing solids from the enzymatically processed material to reduce fluoride content of the material, separating and drying the PPC material. Then, using extraction with supercritical CO.sub.2 or supercritical dimethyl ether, and/or ethanol as solvents, krill oil, inter alia, is separated from the PPC. In the extraction the krill oil can be separated almost wholly from the feed material.

Disclosed is a method for synthesis of fatty acids by culturing a eukaryotic microorganism from the fungi kingdom, that is naturally oleaginous or rendered oleaginous. The culture is performed in the presence a fatty acid synthase inhibitor in the culture medium.

A demulsifying composition is disclosed for aiding extraction of an emulsified oil from an oil and water emulsion. The composition includes one or more non-ionic surfactants having a HLB value of 6 or greater, wherein the non-ionic surfactant is selected from the group consisting of alkoxylated plant oils, alkoxylated plant fats, alkoxylated animal oils, alkoxylated animal fats, alkyl polyglucosides, alkoxylated glycerols, and mixtures thereof. The composition may include silicon containing particles. A method for recovering oil from a corn to ethanol process is also disclosed. The method includes the steps of adding the composition to a process stream of the corn to ethanol process, and extracting oil from the process stream.

A demulsifying composition is disclosed for aiding extraction of an emulsified oil from an oil and water emulsion. The composition includes one or more non-ionic surfactants having a HLB value of 6 or greater, wherein the non-ionic surfactant is selected from the group consisting of alkoxylated plant oils, alkoxylated plant fats, alkoxylated animal oils, alkoxylated animal fats, alkyl polyglucosides, alkoxylated glycerols, and mixtures thereof. The composition may include silicon containing particles. A method for recovering oil from a corn to ethanol process is also disclosed. The method includes the steps of adding the composition to a process stream of the corn to ethanol process, and extracting oil from the process stream.