Provided is a variant strain of the genus Yarrowia. More specifically, provided are a variant strain of the genus Yarrowia, in which activity of phosphatidylethanolamine N-methyltransferase (PEMT) or phospholipid methyltransferase is inactivated, and a method of increasing a fat in the strain, including culturing the strain, or a method of preparing a fat.

The invention relates to a method of extracting oil from plant matter including treating a liquid or liquefied plant material to a high pressure extraction step by contacting the plant material with CO.sub.2 at a pressure between 100 and 3000 psig. And then separating the CO.sub.2 and the extracted oil to form an extracted plant meal. Preferably the extracted plant meal comprises less than 2 wt % of residual oil. The plant material preferably has not been previously extracted with a hydrocarbon solvent and is not heat treated during the extraction step.

The invention relates to a method of extracting oil from plant matter including treating a liquid or liquefied plant material to a high pressure extraction step by contacting the plant material with CO.sub.2 at a pressure between 100 and 3000 psig. And then separating the CO.sub.2 and the extracted oil to form an extracted plant meal. Preferably the extracted plant meal comprises less than 2 wt % of residual oil. The plant material preferably has not been previously extracted with a hydrocarbon solvent and is not heat treated during the extraction step.

Methods for separating lean and fat from beef or other meats and the separation apparatus are shown. The methods use microbiocidal fluids to reduce or eliminate possible sources of contamination.

Methods for separating lean and fat from beef or other meats and the separation apparatus are shown. The methods use microbiocidal fluids to reduce or eliminate possible sources of contamination.

The present disclosure discloses an appliance, suitable for home use, for grinding oil-containing hard produce, in particular oil-containing seeds, to therefore extract an oily substance product. For example, the appliance may grind sesame to obtain Tahini, or peanuts/almonds to obtain butter therefrom. The present disclosure further discloses a capsule to be received in a grinding appliance such as disclosed herein. The appliance incudes two grinding members, configured to grind the hard produce. Each grinding member has a grooved face that is patterned to be configured for grinding. The hard produce is grinded in the interface defined between the two grooved faces, defining a grinding zone. The grinding members typically have a shape of a circle and are aligned one with the other along a common axis. At least one of the grinding members is configured to rotate about the axis to perform the motion carrying out the grinding in the interface between the members. The hard produce is introduced into the grinding zone via one or more openings in at least one of the grinding members. The openings lead directly into the grinding zone, namely the hard produce that is fed through the openings reaches to a part of one of the grooved faces to initiate the grinding process. The grinding zone is constituted by two grinding portions, an inner grinding portion and a peripheral grinding portion, defining inner grinding zone and peripheral grinding zone, respectively. A peripheral collection zone is disposed peripheral to the peripheral grinding zone and is configured to collect the grinded oily substance product that radially flows from the peripheral grinding portions. The oily substance in the collection zone flows towards a substance collector, through which the oily substance flows for being collected.

Lipids can be extracted from a microbial biomass that constitutes at least 20% lipids by weight and has a moisture content of less than 4% by weight by applying pressure to the biomass so as to release lipids therefrom, thereby leaving a biomass of reduced lipid content; and collecting the lipids.

Lipids can be extracted from a microbial biomass that constitutes at least 20% lipids by weight and has a moisture content of less than 4% by weight by applying pressure to the biomass so as to release lipids therefrom, thereby leaving a biomass of reduced lipid content; and collecting the lipids.

A method and apparatus for extracting active components from a plant material by: introducing the material containing the active components in an extraction chamber: introducing a hydrofluorocarbonated compound in a tank at a pressure between 482.6 kPa and 1,447.9 kPa (70 psi to 210 psi): introducing ethanol in a receptacle; mixing the hydrofluorocarbonated compound with ethanol; allowing the mixture to reach the plant material; allowing the mixture enriched with ethanol and the hydrofluorocarbonated compound to flow out of the extraction chamber and into a tank; allowing the ethanol to evaporate and become separated from the residual mixture slower than the hydrofluorocarbonated compound; optionally, the hydrofluorocarbonated compound is recovered by condensing and returning it to the pressurised tank; optionally, the ethanol is recovered by condensing and returning it to the mixing receptacle.

A method and apparatus for extracting active components from a plant material by: introducing the material containing the active components in an extraction chamber: introducing a hydrofluorocarbonated compound in a tank at a pressure between 482.6 kPa and 1,447.9 kPa (70 psi to 210 psi): introducing ethanol in a receptacle; mixing the hydrofluorocarbonated compound with ethanol; allowing the mixture to reach the plant material; allowing the mixture enriched with ethanol and the hydrofluorocarbonated compound to flow out of the extraction chamber and into a tank; allowing the ethanol to evaporate and become separated from the residual mixture slower than the hydrofluorocarbonated compound; optionally, the hydrofluorocarbonated compound is recovered by condensing and returning it to the pressurised tank; optionally, the ethanol is recovered by condensing and returning it to the mixing receptacle.