The present invention provides a culture vessel and a measuring device for measuring a cell sheet thickness nondestructively during culturing. A culture vessel having a lid 4 has a float 8 installed in the interior and the float is structured so as to be uplifted by a culture medium in the culture vessel. Further, a cell sheet thickness measuring device having a sensor head 10 having a light-emitting element 10A and a light-receiving element 10B to detect a positional variation of the float in the vertical direction and a controller 11 to process a signal of the sensor head is configured.

There is provided an incubator including an accommodation space that is shielded from an external environment, an environmental control unit that controls an environment of the inside of the accommodation space, a power supply unit that is provided in the accommodation space and transmits electric power to a power supply target in a noncontact state, and a partition wall that is provided in the accommodation space and shields electromagnetic waves radiated from the power supply unit.

Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well.

Provided in the present invention is an incubator capable of producing pressure fluctuations. The incubator comprises an incubator body and a variable pressure apparatus, the incubator body having a variable pressure interface, the variable pressure apparatus being connected to the incubator body by means of the variable pressure interface, and the variable pressure apparatus being used for inputting fluctuating or constant air pressure into the incubator body.

A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.

A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.

A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.

A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.

A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.