The invention relates to a device for isolating stem cells from fetal tissues, which device has an incubation chamber, at least one pump, at least one reservoir for a tissue break-down solution, at least one reservoir for a rinsing solution, optionally a control unit, optionally a means for removing contaminants, and optionally a means for expansion of the isolated stem cells. The invention further relates to a method for isolating stem cells from fetal tissue, which method comprises, among other things, the mechanical dissociation and the enzymatic digestion of the fetal tissue and optionally density gradient centrifugation for removing contaminants. The device and the method according to the invention are particularly suitable for isolating mesenchymal stem cells from fetal tissues, such as umbilical cord tissue, placenta tissue, or fetal lung tissue.

The present set of embodiments relate to a system, method, and apparatus controlling a cell culture media mixing system. The control system includes an integrated control unit capable of controlling a broad ranges of peripheral devices commonly used in bioproduction through a graphical user interface displayed on a touch sensitive screen. The bioproduction system is designed to be highly customizable through process modification and recipe creation by user with little or no knowledge of programming and is capable of controlling a wide variety of devices using a single unit. The bioproduction system allows for auto-detection, auto-calibration, and automatic of device related processes into a bioproduction workflow.

An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

An apparatus comprising an actuator for moving an actuator rod and a bioreactor vessel is disclosed herein. The bioreactor vessel comprises a container for holding a liquid, a mounting for mounting a tissue sample in the container, and, an actuator coupling to enable the actuator rod to be connected for applying mechanical force to the tissue sample. The apparatus also comprises a seat, fixed with respect to the actuator and configured for locating the reactor vessel in a location selected so that the actuator can be connected for applying said force via the actuator coupling. The reactor vessel is removable from the apparatus.

The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.

A process is provided for isolating and analyzing microorganisms contained in a sample by collecting a determined volume in a sample, the determined volume representing all or part of this sample, likely to contain at least one microorganism. The collected volume is then split up into a plurality of compartments having a culture medium, the volume of each compartment being smaller than 10 μL, each compartment being isolated from the other compartments and having no interface with the ambient atmosphere. At least one microorganism is incubated in the compartments for determined durations, and compartments are detected that contain at least one microorganism. The incubation may be extended after detecting compartments so that at least one microorganism having a defined quantity can be detected, and then the content of the detected compartments can be recovered. Finally, at least one functional parameter relative to a microorganism in the compartments is determined.

A biogas reactor is disclosed in which biogas is formed from organic matter. The biogas reactor includes a wall to form a tank structure enclosing a tubular reactor space in which organic matter can be placed to form biogas. The wall forming the tank structure is comprised of a helically wound hollow pipe profile to form a spiral arrangement. The hollow space within the hollow pipe profile accommodates a heat-transfer medium to influence the temperature of organic matter in the reactor space. Connections are provided for feeding organic matter to the reactor space and removing it from the reactor space, and one or more connections are provided for removing biogas from the reactor space. The invention also concerns methods for producing biogas and for manufacturing a biogas reactor.

A cell culture apparatus is provided. A cell culture apparatus according to one exemplary embodiment of the present invention comprises: a cell culture part comprising an accommodation space in which a plurality of supports for cell culture are disposed; a medium supply part for storing a predetermined amount of medium to be supplied to the cell culture part, and maintaining a predetermined carbon dioxide concentration of the medium stored therein by using carbon dioxide introduced from the outside through a gas supply port; and a pump, which interconnects the cell culture part and the medium supply part so as to circulate, in the cell culture part, a medium stored in the medium supply part.

A movable cell incubator contains: a body, a first lid, a second lid and an electric control unit. The body includes a first internal space, a refrigeration room, and an airtight culture room. The first lid airtightly covers the culture room, the second lid airtightly covers the refrigeration room, and the control unit includes a microprocessor, a power module, a digital/analog conversion module defined between a microprocessor and the power module, a heating module controlling temperature of the culture room, a cooling module supplying cold source to the refrigeration room, a peristaltic pump module, a flow sensing module, a CO2 detective supply module supplying CO2 to the culture room, and a setting display module exposing and fixed on the first lid, with the peristaltic pump module aseptically connected between cell culture media and cell culture bag by multiple conveying tubes.

The present disclosure provides methods and materials for the cultivation and/or propagation of a photosynthetic organism. Such methods may comprise the use of a lamp assembly that comprises a plurality of circuit boards, each comprising at least three edges, arranged in a substantially spherical shape defining an interior lamp assembly volume, wherein the plurality of circuit boards comprise a first planar surface in contact with the interior lamp assembly volume and an opposing second planar surface comprising light emitting diodes (LEDs); and a barrier that surrounds the plurality of circuit boards forming the substantially spherical shape.