Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

A scaffold for cell culture or tissue engineering is provided. The scaffold includes a fiber web having a three-dimensional network structure, which includes a biodegradable scaffold fiber. Therefore, a microenvironment suitable for migration, proliferation and differentiation of cells to be cultured is created, thereby improving a cell proliferation rate and cell viability. In addition, the scaffold may be easily removed from cells cultured therein without physical/chemical stimuli, and thus the cultured cells may be easily recovered, and is able to be grafted into the body while the cultured cells are included in the scaffold. Moreover, the cultured cells may be cultured to have a similar shape/structure to those of an actual animal body to make it more suitable to be applied in grafting into an in vitro experimental model or animal body.

An algae cultivation system may include: a plurality of panels within a cultivation container, positioned along a first axis perpendicular to the gravitational force, wherein a cultivation volume is created between each pair of panels, and wherein the cultivation volumes are fluidly coupled so as to allow horizontal flow therebetween along the first axis; at least one first sparger, to distribute a first fluid into the container at a first operating flow rate; at least one second sparger, to distribute a second fluid into the container at a second operating flow rate; and at least one controller, to control the first operating flow rate and the second operating flow rate. The first operating flow rate may be adapted to allow turbulent mixing the algae in the cultivation container, and the second operating flow rate may be adapted to allow assimilation of materials in a liquid in the cultivation container.

Zebrafish are a powerful model for investigating cardiac repair due to their unique regenerative abilities, scalability, and compatibility with many genetic tools. However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque and explanted hearts in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. Unlike hearts cultured in dishes for one week, the morphology and calcium activity of hearts cultured in the device for one week were largely similar to freshly explanted hearts. We also cultured injured hearts in the device and used live imaging techniques to continuously record the revascularization process over several days, demonstrating how our device enables unprecedented visual access to the multi-day process of adult zebrafish heart regeneration.

A device and method for achieving RNAi of insects by atomization are provided. The device includes an insect placing box installed on a chemical solution atomizer; a power plug is provided at one side of the chemical solution atomizer, an atomization release mechanism is provided in the chemical solution atomizer and is in communication with the insect placing box. The atomization release mechanism includes an atomized gas release port formed on the insect placing box, an ultrasonic atomizer arranged below the atomized gas release port, a suction tube, a chemical solution storage chamber connected to an end of the suction tube, and a piston installed on the chemical solution storage chamber. One sidewall surface of the chemical solution atomizer is an operation panel suitable for controlling atomization. An atomization regulating switch is installed on the operation panel.

A device and method for achieving RNAi of insects by atomization are provided. The device includes an insect placing box installed on a chemical solution atomizer; a power plug is provided at one side of the chemical solution atomizer, an atomization release mechanism is provided in the chemical solution atomizer and is in communication with the insect placing box. The atomization release mechanism includes an atomized gas release port formed on the insect placing box, an ultrasonic atomizer arranged below the atomized gas release port, a suction tube, a chemical solution storage chamber connected to an end of the suction tube, and a piston installed on the chemical solution storage chamber. One sidewall surface of the chemical solution atomizer is an operation panel suitable for controlling atomization. An atomization regulating switch is installed on the operation panel.

The present disclosure provides systems and methods for recycling gas in a reactor. An example of gas-recycling system comprises: a housing, a gas conduit, and a powered propeller. The housing encloses a gas space and a liquid space, wherein the gas space is configured to collect gas within a reactor. The powered propeller comprises a shaft having an upper end and a lower end; and a plurality of radial blades connected to the lower end of the shaft. Upon rotation of the powered propeller, the powered propeller is configured to: generate a suction to cause the collected gas to flow from the gas space to the liquid space through the conduit; cause fluid of the reactor to flow in a direction from the upper end of the shaft to the lower end of the shaft; and mix the liquid and the collected gas proximate the powered propeller.

There is described a fermentation tank for the multiplication of microorganisms, particularly a vertical fermentation tank comprising aeration and agitation elements with low cost and high efficiency. Particularly, a fermentation tank for the multiplication of microorganisms (100) comprising an external structure (10) of longitudinal axis (L) and an inner container (20) which receives a mixture to be fermented, the fermentation tank (100) comprising an aeration and agitation element (30) arranged in the inner container (20) collinear with the longitudinal axis (L), the aeration and agitation element (30) comprising internally a plurality of sets of fins (40), each set of fins (40) being arranged axially parallel to each other along the longitudinal axis (L) to provide aeration and agitation of the mixture to be fermented.

A sample testing apparatus includes a sample tray defining a planar surface and a plurality of wells recessed relative to the planar surface, and a lid member configured to be sealed about the planar surface of the sample tray. The lid member includes an adhesive layer configured to be sealed to the planar surface of the sample tray, a breathable film layer disposed about the adhesive layer, and a backing layer disposed about the breathable film layer. Methods of using the sample testing apparatus for testing a sample and kits to facilitate such testing are also provided.

The present invention is a manufacturing method for producing fermentation products using a fermentation vessel, including steps of: preparing a fermentation vessel and a sensor, introducing liquid into the fermentation vessel, and operating the fermentation vessel in which properties of liquid in the fermentation vessel are measured to adjust operating conditions; wherein the sensor device has a sensor for measuring liquid properties and a sensor cover body; a bottom permeable portion for passing liquid and crystals in the liquid is disposed on the bottom surface of the cover body, and a top permeable portion for passing liquid and crystals in the liquid is disposed on the top surface of the cover body; micropores are respectively formed in the bottom permeable portion and the top permeable portion; and micropores disposed in the top permeable portion are the same or larger than micropores disposed in the top permeable portion.