An object of the present invention is to provide a transportation instrument and a transportation method that enable transportation by using a cell culture container as it is. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable supply of oxygen required for respiration of cells. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable suppression of migration of a culture medium into and out of cells caused by the influence of an increase in internal pressure with respect to the temperature during transportation. According to the present invention, provided is a cell preservation or transportation instrument including a plurality of cell storage containers, a plate which has a plurality of recesses for holding the plurality of cell storage containers therein, and a flexible material sheet which seals upper opening portions of the plurality of cell storage containers and upper side wall portions formed by side walls of the recesses of the plate and allows ventilation between an inside and an outside of the plurality of cell storage containers.

Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.

Primary mammalian cell culture systems, methods of making primary mammalian cell culture systems, and methods of culturing primary mammalian cells are provided. In some embodiments the systems and methods utilize an exothermic chemical process as a heat source for culturing and shipping cultures comprising primary mammalian cells.

A sparger assembly for a bioprocessing system includes a first layer having a plurality of pores of a first size, and a second layer disposed above the first layer and having a plurality of holes of a second size, the second size being greater than the first size. The pores of the first layer and the holes of the second layer allow for the passage of a sparge gas through the first layer and the second layer.

A sample testing apparatus includes a sample tray defining a planar surface and a plurality of wells recessed relative to the planar surface, and a lid member configured to be sealed about the planar surface of the sample tray. The lid member includes an adhesive layer configured to be sealed to the planar surface of the sample tray, a breathable film layer disposed about the adhesive layer, and a backing layer disposed about the breathable film layer. Methods of using the sample testing apparatus for testing a sample and kits to facilitate such testing are also provided.

A method and system of and for handling, preserving, separating, filtering, collecting, manipulating, and/or culturing ex vivo biological material including red blood cells, white blood cells, and blood plasma within a bioreactor having a gas permeable membrane that allows for passive ventilation.

Disclosed herein is a method for assisting a thermally-induced change to a nano-sized solute or dispersed-phase in a liquid. The method comprises the step of passing gas bubbles through the liquid, the gas in the gas bubbles having a temperature higher than the bulk temperature of the liquid.

Disclosed herein is a method for assisting a thermally-induced change to a nano-sized solute or dispersed-phase in a liquid. The method comprises the step of passing gas bubbles through the liquid, the gas in the gas bubbles having a temperature higher than the bulk temperature of the liquid.

Microfluidic gas exchange devices may include one or more exchange modules (100), wherein each exchange module includes: a first layer comprising: one or more primary inlets (108); a first capillary network connected to the one or more primary inlets, wherein the first capillary network extends radially outward from at least one of the one or more primary inlets, and wherein the first capillary network includes one or more injection branches (104) and a series of microchannels (106); and one or more primary outlets connected to the first capillary network; and a second layer that includes a semipermeable membrane.

The present invention provides improved bioprocessing systems and methods for cell culture using the improved bioreactors, e.g., batch-fed or perfusion bioreactor cell culture systems for production of monoclonal or bi-specific antibodies, which are modified to include one or more membrane gas transfer modules in place of a sparger- or microsparger-based aeration systems to better regulate the levels of critical gases in a bioreactor cell culture, e.g., the dissolved levels of O2 and CO2, even at high cell densities, without subjecting the cells to bubble-burst associated cell death.