A cell bag rotator is constructed to slowly agitate a plurality of cell bags by rotation along a rotation axis that is at least partially horizontal. The cell bag rotator comprises a plurality of plates to which a respective cell bag is attached. The cell bag rotator is suitable for mixing cells with particles for performing various types of subsequent cell therapy processes on the cells.

A cell bag rotator is constructed to slowly agitate a plurality of cell bags by rotation along a rotation axis that is at least partially horizontal. The cell bag rotator comprises a plurality of plates to which a respective cell bag is attached. The cell bag rotator is suitable for mixing cells with particles for performing various types of subsequent cell therapy processes on the cells.

A method for operating a bioreactor includes passing a drive shaft into a first tubular connector and a second tubular connector projecting from the first tubular connector, the first tubular connector and the second tubular connector being at least partially disposed within a container, the first tubular connector being more flexible than the second tubular connector, the second tubular connector having a length that comprises at least 20% of a combined length of the first tubular connector and the second tubular connector. Rotating the drive shaft so as to rotate the first tubular connector and the second tubular connector within the container.

A method for operating a bioreactor includes passing a drive shaft into a first tubular connector and a second tubular connector projecting from the first tubular connector, the first tubular connector and the second tubular connector being at least partially disposed within a container, the first tubular connector being more flexible than the second tubular connector, the second tubular connector having a length that comprises at least 20% of a combined length of the first tubular connector and the second tubular connector. Rotating the drive shaft so as to rotate the first tubular connector and the second tubular connector within the container.

The present disclosure provides a delivery consumable for delivering a fluid to a bioreactor. The delivery consumable comprises a container including a collapsible portion comprising a collapsible wall, and an intermediate portion connected to the collapsible portion and being adapted to hold a fluid. The deliver consumable also comprises a connector adapted to connect the intermediate portion to the bioreactor. The collapsible portion is adapted to collapse and urge the fluid through the connector and into the bioreactor.

An assembly for handling biological material, including a planar interface having at least one port, and a component retaining element, spaced apart from the planar interface, configured to retain a component for handling biological material, in which at least one component chosen from the planar interface and the component retaining element is moveable to bring the port and the component retaining element into registration with one another. The registration between the at least one port and the component retaining element adapted to form a fluid communication pathway may be provided between a component retained by the component retaining element and a container associated with the planar interface through the port.

An assembly for handling biological material, including a planar interface having at least one port, and a component retaining element, spaced apart from the planar interface, configured to retain a component for handling biological material, in which at least one component chosen from the planar interface and the component retaining element is moveable to bring the port and the component retaining element into registration with one another. The registration between the at least one port and the component retaining element adapted to form a fluid communication pathway may be provided between a component retained by the component retaining element and a container associated with the planar interface through the port.

A cell bag rotator is constructed to slowly agitate a plurality of cell bags by rotation along a rotation axis that is at least partially horizontal. The cell bag rotator comprises a plurality of plates to which a respective cell bag is attached. The cell bag rotator is suitable for mixing cells with particles for performing various types of subsequent cell therapy processes on the cells.

A cell bag rotator is constructed to slowly agitate a plurality of cell bags by rotation along a rotation axis that is at least partially horizontal. The cell bag rotator comprises a plurality of plates to which a respective cell bag is attached. The cell bag rotator is suitable for mixing cells with particles for performing various types of subsequent cell therapy processes on the cells.

A polymeric substrate has a plasma treated contact surface which is roughened, hydrophilic and has a higher oxygen atomic content than the interior portion. The treated contact surface has an enhanced cell adhesion, cell growth and cell recovery rate. During the treatment, the contact surface is contacted with a process gas introduced through a gas inlet near the treated surface and radio frequency electrical power is introduced in the process gas, forming a treated contact surface that has improved cell recovery compared to an untreated contact surface. The process gas optionally can be nitrogen gas, oxygen gas, or a gas that contains nitrogen atoms, oxygen atoms, or a combination of nitrogen and oxygen atoms. The process optionally improves cell recovery of a chicken embryo cell culture from the treated contact surface.